DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma
Hypermethylation of specific gene promoters is an important mechanism of carcinogenesis. A high frequency of promoter methylation of PAX1 and ZNF582 genes has been detected in cervical cancer. In the present study, we investigated the methylation status of PAX1 and ZNF582 genes in esophageal squamou...
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doaj-8a39f73a72024b55b6f6025bc96224002020-11-24T21:02:56ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012017-02-0114221610.3390/ijerph14020216ijerph14020216DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell CarcinomaJin Huang0Guo Wang1Jie Tang2Wei Zhuang3Li-Ping Wang4Yu-Ligh Liou5Ying-Zi Liu6Hong-Hao Zhou7Yuan-Shan Zhu8Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaDepartment of Cardiovascular & Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Clinical Oncology, The First People’s Hospital of Chenzhou, Chenzhou 423000, Hunan, ChinaDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaDepartment of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, ChinaHypermethylation of specific gene promoters is an important mechanism of carcinogenesis. A high frequency of promoter methylation of PAX1 and ZNF582 genes has been detected in cervical cancer. In the present study, we investigated the methylation status of PAX1 and ZNF582 genes in esophageal squamous cell carcinoma (ESCC) tissues. Tumor and paracancerous tissues were obtained from 14 ESCC patients. Genomic DNA was extracted from both tumor and paracancerous tissues, and the concentration of DNA were determined. DNA methylation analysis of PAX1 and ZNF582 genes was carried out using quantitative methylation-specific PCR. To assess the diagnostic performance of the two methylated genes for cancer detection, receiver operating characteristic (ROC) curves were generated. Sensitivities and specificities were tested at cut-offs obtained from the ROC curves. The methylation levels of both PAX1 and ZNF582 genes were significantly higher in tumor tissues compared to non-tumor paracancerous tissues. The methylation rates of PAX1 and ZNF582 in ESCC tumor and paracancerous tissues were 100% and 21.4% (p = 0.006), 85.7% and 0% (p < 0.001), respectively. The sensitivities and specificities of PAX1 and ZNF582 methylation for the detection of cancer were 100% and 85.7%, and 78.6% and 100%, respectively. The DNA methylation levels and frequencies of PAX1 and ZNF582 genes were markedly higher in ESCC tumor tissues compared to those in paracancerous tissues. Moreover, the conclusions were verified by using The Cancer Genome Atlas (TCGA) datasets. DNA methylation status of these two genes showed a relatively good sensitivity and specificity for the detection of ESCC tumors. This data suggests that DNA methylation testing holds a great promise for ESCC screening and warrants further prospective population-based studies.http://www.mdpi.com/1660-4601/14/2/216esophageal squamous cell carcinomaDNA methylationPAX1ZNF582 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Huang Guo Wang Jie Tang Wei Zhuang Li-Ping Wang Yu-Ligh Liou Ying-Zi Liu Hong-Hao Zhou Yuan-Shan Zhu |
spellingShingle |
Jin Huang Guo Wang Jie Tang Wei Zhuang Li-Ping Wang Yu-Ligh Liou Ying-Zi Liu Hong-Hao Zhou Yuan-Shan Zhu DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma International Journal of Environmental Research and Public Health esophageal squamous cell carcinoma DNA methylation PAX1 ZNF582 |
author_facet |
Jin Huang Guo Wang Jie Tang Wei Zhuang Li-Ping Wang Yu-Ligh Liou Ying-Zi Liu Hong-Hao Zhou Yuan-Shan Zhu |
author_sort |
Jin Huang |
title |
DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma |
title_short |
DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma |
title_full |
DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma |
title_fullStr |
DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma |
title_full_unstemmed |
DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma |
title_sort |
dna methylation status of pax1 and znf582 in esophageal squamous cell carcinoma |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2017-02-01 |
description |
Hypermethylation of specific gene promoters is an important mechanism of carcinogenesis. A high frequency of promoter methylation of PAX1 and ZNF582 genes has been detected in cervical cancer. In the present study, we investigated the methylation status of PAX1 and ZNF582 genes in esophageal squamous cell carcinoma (ESCC) tissues. Tumor and paracancerous tissues were obtained from 14 ESCC patients. Genomic DNA was extracted from both tumor and paracancerous tissues, and the concentration of DNA were determined. DNA methylation analysis of PAX1 and ZNF582 genes was carried out using quantitative methylation-specific PCR. To assess the diagnostic performance of the two methylated genes for cancer detection, receiver operating characteristic (ROC) curves were generated. Sensitivities and specificities were tested at cut-offs obtained from the ROC curves. The methylation levels of both PAX1 and ZNF582 genes were significantly higher in tumor tissues compared to non-tumor paracancerous tissues. The methylation rates of PAX1 and ZNF582 in ESCC tumor and paracancerous tissues were 100% and 21.4% (p = 0.006), 85.7% and 0% (p < 0.001), respectively. The sensitivities and specificities of PAX1 and ZNF582 methylation for the detection of cancer were 100% and 85.7%, and 78.6% and 100%, respectively. The DNA methylation levels and frequencies of PAX1 and ZNF582 genes were markedly higher in ESCC tumor tissues compared to those in paracancerous tissues. Moreover, the conclusions were verified by using The Cancer Genome Atlas (TCGA) datasets. DNA methylation status of these two genes showed a relatively good sensitivity and specificity for the detection of ESCC tumors. This data suggests that DNA methylation testing holds a great promise for ESCC screening and warrants further prospective population-based studies. |
topic |
esophageal squamous cell carcinoma DNA methylation PAX1 ZNF582 |
url |
http://www.mdpi.com/1660-4601/14/2/216 |
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