Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’
Studies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of <i>Actinidia eriantha</i>. Fruits of ‘Ganmi 6’ harvested at eleven developmental periods were used a...
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doaj-8a52bb7b68a241f989e14cca1e82da802020-11-25T02:57:37ZengMDPI AGPlants2223-77472020-03-019333210.3390/plants9030332plants9030332Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’Zhiqiang Jiang0Qing Huang1Dongfeng Jia2Min Zhong3Junjie Tao4Guanglian Liao5Chunhui Huang6Xiaobiao Xu7Institute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, ChinaStudies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of <i>Actinidia eriantha</i>. Fruits of ‘Ganmi 6’ harvested at eleven developmental periods were used as materials. The components and content of organic acids were determined by high-performance liquid chromatography (HPLC) system, the activities of the related enzyme were detected, and gene expression levels were measured by quantitative real-time PCR (qRT-PCR). Components of ascorbic acid (AsA) and eight kinds of organic acids were detected. These results showed that quinic acid and citric acid were the main organic acids in the fruit of ‘Ganmi 6’. Correlation analysis showed that NADP-Quinate dehydrogenase (NADP-QDH), NADP-Shikimate dehydrogenase (NADP-SDH), and Cyt-Aconitase (Cyt-Aco) may be involved in regulating organic acids biosynthesis. Meanwhile, the <i>SDH</i> gene may play an important role in regulating the accumulation of citric acid. In this study, the activities of NADP-SDH, Mit-Aconitase (Mit-Aco), and NAD-Isocitrate dehydrogenase (NAD-IDH) were regulated by their corresponding genes at the transcriptional level. The activity of Citrate synthase (CS) may be affected by post-translational modification. Our results provided new insight into the characteristics of organic acid metabolism in the fruit of <i>A. eriantha</i>.https://www.mdpi.com/2223-7747/9/3/332<i>actinidia eriantha</i>‘ganmi 6’organic acidenzyme activitygene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhiqiang Jiang Qing Huang Dongfeng Jia Min Zhong Junjie Tao Guanglian Liao Chunhui Huang Xiaobiao Xu |
spellingShingle |
Zhiqiang Jiang Qing Huang Dongfeng Jia Min Zhong Junjie Tao Guanglian Liao Chunhui Huang Xiaobiao Xu Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’ Plants <i>actinidia eriantha</i> ‘ganmi 6’ organic acid enzyme activity gene expression |
author_facet |
Zhiqiang Jiang Qing Huang Dongfeng Jia Min Zhong Junjie Tao Guanglian Liao Chunhui Huang Xiaobiao Xu |
author_sort |
Zhiqiang Jiang |
title |
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’ |
title_short |
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’ |
title_full |
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’ |
title_fullStr |
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’ |
title_full_unstemmed |
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of <i>Actinidia eriantha</i> ‘Ganmi 6’ |
title_sort |
characterization of organic acid metabolism and expression of related genes during fruit development of <i>actinidia eriantha</i> ‘ganmi 6’ |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-03-01 |
description |
Studies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of <i>Actinidia eriantha</i>. Fruits of ‘Ganmi 6’ harvested at eleven developmental periods were used as materials. The components and content of organic acids were determined by high-performance liquid chromatography (HPLC) system, the activities of the related enzyme were detected, and gene expression levels were measured by quantitative real-time PCR (qRT-PCR). Components of ascorbic acid (AsA) and eight kinds of organic acids were detected. These results showed that quinic acid and citric acid were the main organic acids in the fruit of ‘Ganmi 6’. Correlation analysis showed that NADP-Quinate dehydrogenase (NADP-QDH), NADP-Shikimate dehydrogenase (NADP-SDH), and Cyt-Aconitase (Cyt-Aco) may be involved in regulating organic acids biosynthesis. Meanwhile, the <i>SDH</i> gene may play an important role in regulating the accumulation of citric acid. In this study, the activities of NADP-SDH, Mit-Aconitase (Mit-Aco), and NAD-Isocitrate dehydrogenase (NAD-IDH) were regulated by their corresponding genes at the transcriptional level. The activity of Citrate synthase (CS) may be affected by post-translational modification. Our results provided new insight into the characteristics of organic acid metabolism in the fruit of <i>A. eriantha</i>. |
topic |
<i>actinidia eriantha</i> ‘ganmi 6’ organic acid enzyme activity gene expression |
url |
https://www.mdpi.com/2223-7747/9/3/332 |
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