Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C

• Main Authors: O. S. Oliinyk, K. O. Palyvoda, N. E. Lugovskaya, D. V. Kolibo, E. V. Lugovskoy, S. V. Komisarenko
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2015-04-01
• Series: Ukrainian Biochemical Journal
• Subjects: phage display; protein C; scFv antibodies
• Online Access: http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Oliinyk_2_15.pdf

The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein C was synthesized and the murine immune scFv library against this peptide was constructed. The protein C specific scFv 9E were selected from the constructed library by the phage-display method. The scFv 9E dissociation constant was found to be 2∙10-9 М. It was shown that scFv 9E were suitable for protein C detection by ELISA and Western blotting. Selected scFv could be further used for protein C investigation and for the development of quantitative methods for protein C detection in human blood.