Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin
Mesoporous silica nanoparticles (MSNs) with ordered pore structure have been synthesized and used as carriers for the anticancer drug curcumin. MSNs were functionalized with amine groups and further attached with carboxymethyl cellulose (CMC) using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC...
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doaj-8a7d9f6711c040c5812bbd297cc93bae2020-11-24T20:45:32ZengMDPI AGGels2310-28612017-02-0131810.3390/gels3010008gels3010008Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of CurcuminNeha Tiwari0Laxman Nawale1Dhiman Sarkar2Manohar V. Badiger3Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune 411008, IndiaCombichem Bioresource Centre, Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune 411008, IndiaAcademy of Scientific & Innovative Research, CSIR-NCL Campus, Pune 411008, IndiaPolymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune 411008, IndiaMesoporous silica nanoparticles (MSNs) with ordered pore structure have been synthesized and used as carriers for the anticancer drug curcumin. MSNs were functionalized with amine groups and further attached with carboxymethyl cellulose (CMC) using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) coupling chemistry, which increased the hydrophilicity and biocompatibility of MSNs. The functionalized MSNs (MSN-NH2 and MSN-CMC) were characterized using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), N2 adsorption, X-Ray Diffraction (XRD), Thermo Gravimetric Analysis (TGA) and Fourier Transform Infrared Spectroscopy (FT-IR). The in vitro release of curcumin from the –NH2 and CMC functionalized MSNs (MSN-cur-NH2 and MSN-cur-CMC) was performed in 0.5% aqueous solution of sodium lauryl sulphate (SLS). The effect of CMC functionalization of MSNs towards cellular uptake was studied in the human breast cancer cell line MDA-MB-231 and was compared with that of MSN-NH2 and free curcumin (cur). Both MSN-NH2 and MSN-CMC showed good biocompatibility with the breast cancer cell line. The MTT assay study revealed that curcumin-loaded MSN-cur-CMC showed better uptake as compared to curcumin-loaded MSN-cur-NH2. Free curcumin was used as a control and was shown to have much less internalization as compared to the curcumin-loaded functionalized MSNs due to poor bioavailability. Fluorescence microscopy was used to localize the fluorescent drug curcumin inside the cells. The work demonstrates that CMC-functionalized MSNs can be used as potential carriers for loading and release of hydrophobic drugs that otherwise cannot be used effectively in their free form for cancer therapy.http://www.mdpi.com/2310-2861/3/1/8curcuminmesoporous silica nanoparticlescarboxymethyl cellulosedrug delivery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neha Tiwari Laxman Nawale Dhiman Sarkar Manohar V. Badiger |
spellingShingle |
Neha Tiwari Laxman Nawale Dhiman Sarkar Manohar V. Badiger Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin Gels curcumin mesoporous silica nanoparticles carboxymethyl cellulose drug delivery |
author_facet |
Neha Tiwari Laxman Nawale Dhiman Sarkar Manohar V. Badiger |
author_sort |
Neha Tiwari |
title |
Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin |
title_short |
Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin |
title_full |
Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin |
title_fullStr |
Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin |
title_full_unstemmed |
Carboxymethyl Cellulose-Grafted Mesoporous Silica Hybrid Nanogels for Enhanced Cellular Uptake and Release of Curcumin |
title_sort |
carboxymethyl cellulose-grafted mesoporous silica hybrid nanogels for enhanced cellular uptake and release of curcumin |
publisher |
MDPI AG |
series |
Gels |
issn |
2310-2861 |
publishDate |
2017-02-01 |
description |
Mesoporous silica nanoparticles (MSNs) with ordered pore structure have been synthesized and used as carriers for the anticancer drug curcumin. MSNs were functionalized with amine groups and further attached with carboxymethyl cellulose (CMC) using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) coupling chemistry, which increased the hydrophilicity and biocompatibility of MSNs. The functionalized MSNs (MSN-NH2 and MSN-CMC) were characterized using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), N2 adsorption, X-Ray Diffraction (XRD), Thermo Gravimetric Analysis (TGA) and Fourier Transform Infrared Spectroscopy (FT-IR). The in vitro release of curcumin from the –NH2 and CMC functionalized MSNs (MSN-cur-NH2 and MSN-cur-CMC) was performed in 0.5% aqueous solution of sodium lauryl sulphate (SLS). The effect of CMC functionalization of MSNs towards cellular uptake was studied in the human breast cancer cell line MDA-MB-231 and was compared with that of MSN-NH2 and free curcumin (cur). Both MSN-NH2 and MSN-CMC showed good biocompatibility with the breast cancer cell line. The MTT assay study revealed that curcumin-loaded MSN-cur-CMC showed better uptake as compared to curcumin-loaded MSN-cur-NH2. Free curcumin was used as a control and was shown to have much less internalization as compared to the curcumin-loaded functionalized MSNs due to poor bioavailability. Fluorescence microscopy was used to localize the fluorescent drug curcumin inside the cells. The work demonstrates that CMC-functionalized MSNs can be used as potential carriers for loading and release of hydrophobic drugs that otherwise cannot be used effectively in their free form for cancer therapy. |
topic |
curcumin mesoporous silica nanoparticles carboxymethyl cellulose drug delivery |
url |
http://www.mdpi.com/2310-2861/3/1/8 |
work_keys_str_mv |
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1716814377450995712 |