Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells
Background/Aims: Human embryonic stem cells (hESCs) are a potential source of cells for treatment of many degenerative diseases, but in culture have a propensity to spontaneously differentiate, possibly due to suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regula...
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Cell Physiol Biochem Press GmbH & Co KG
2014-01-01
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doaj-8a8142aca9634599befcc73636cf9e9a2020-11-25T01:27:36ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-01-0133123724610.1159/000356665356665Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem CellsDavid R. ChristensenPhilip C. CalderFranchesca D. HoughtonBackground/Aims: Human embryonic stem cells (hESCs) are a potential source of cells for treatment of many degenerative diseases, but in culture have a propensity to spontaneously differentiate, possibly due to suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regulates carbohydrate metabolism. Hence, a greater understanding of the nutrient requirements of hESCs will allow production of more appropriate culture media. This study aims to investigate the effect of environmental oxygen tension on the amino acid metabolism of hESCs. Methods: The production or depletion of amino acids by hESCs cultured at 5% or 20% oxygen in the presence or absence of FGF2 was measured by reversephase HPLC. Results: Atmospheric oxygen, or removal of FGF2 from hESCs cultured at 5% oxygen, perturbed the uptake or release of individual amino acids and the total amino acid turnover compared to hESCs cultured at 5% oxygen. In particular, serine uptake was reduced at 20% oxygen and by removal of FGF2. Conclusions: Highly pluripotent hESCs, cultured at 5% oxygen, demonstrate a greater amino acid turnover than hESCs cultured at 20% oxygen, or without FGF2. These data suggest that amino acid turnover could be used as a measure of the self-renewal capacity of hESCs.http://www.karger.com/Article/FullText/356665Embryonic stem cellsAmino acidsHypoxiaOxygen tensionMetabolism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David R. Christensen Philip C. Calder Franchesca D. Houghton |
spellingShingle |
David R. Christensen Philip C. Calder Franchesca D. Houghton Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells Cellular Physiology and Biochemistry Embryonic stem cells Amino acids Hypoxia Oxygen tension Metabolism |
author_facet |
David R. Christensen Philip C. Calder Franchesca D. Houghton |
author_sort |
David R. Christensen |
title |
Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells |
title_short |
Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells |
title_full |
Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells |
title_fullStr |
Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells |
title_full_unstemmed |
Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells |
title_sort |
effect of oxygen tension on the amino acid utilisation of human embryonic stem cells |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2014-01-01 |
description |
Background/Aims: Human embryonic stem cells (hESCs) are a potential source of cells for treatment of many degenerative diseases, but in culture have a propensity to spontaneously differentiate, possibly due to suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regulates carbohydrate metabolism. Hence, a greater understanding of the nutrient requirements of hESCs will allow production of more appropriate culture media. This study aims to investigate the effect of environmental oxygen tension on the amino acid metabolism of hESCs. Methods: The production or depletion of amino acids by hESCs cultured at 5% or 20% oxygen in the presence or absence of FGF2 was measured by reversephase HPLC. Results: Atmospheric oxygen, or removal of FGF2 from hESCs cultured at 5% oxygen, perturbed the uptake or release of individual amino acids and the total amino acid turnover compared to hESCs cultured at 5% oxygen. In particular, serine uptake was reduced at 20% oxygen and by removal of FGF2. Conclusions: Highly pluripotent hESCs, cultured at 5% oxygen, demonstrate a greater amino acid turnover than hESCs cultured at 20% oxygen, or without FGF2. These data suggest that amino acid turnover could be used as a measure of the self-renewal capacity of hESCs. |
topic |
Embryonic stem cells Amino acids Hypoxia Oxygen tension Metabolism |
url |
http://www.karger.com/Article/FullText/356665 |
work_keys_str_mv |
AT davidrchristensen effectofoxygentensionontheaminoacidutilisationofhumanembryonicstemcells AT philipccalder effectofoxygentensionontheaminoacidutilisationofhumanembryonicstemcells AT franchescadhoughton effectofoxygentensionontheaminoacidutilisationofhumanembryonicstemcells |
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1725104355289333760 |