Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine
Current restrictions on the use of antibiotics, associated with increases in bacterial resistance, require new solutions, including materials with antibacterial properties. In this study, copper alginate fibers obtained using the classic wet method were used to make nonwovens which were modified wit...
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| Online Access: | https://www.mdpi.com/1996-1944/13/2/337 |
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doaj-8a8f1a72ed7c429c8ad1b9750ad757f82020-11-25T03:35:56ZengMDPI AGMaterials1996-19442020-01-0113233710.3390/ma13020337ma13020337Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative MedicineJustyna Fraczyk0Joanna Wasko1Malgorzata Walczak2Zbigniew J. Kaminski3Dorota Puchowicz4Irena Kaminska5Maciej Bogun6Marcin Kolasa7Ewa Stodolak-Zych8Anna Scislowska-Czarnecka9Beata Kolesinska10Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, PolandInstitute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, PolandInstitute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, PolandInstitute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, PolandLukasiewicz Research Network-Textile Research Institute, Brzezinska 5/15, 92-103 Lodz, PolandLukasiewicz Research Network-Textile Research Institute, Brzezinska 5/15, 92-103 Lodz, PolandLukasiewicz Research Network-Textile Research Institute, Brzezinska 5/15, 92-103 Lodz, PolandMilitary Institute of Hygiene and Epidemiology Department of Pharmacology and Toxicology, Kozielska 4, 01-163 Warsaw, PolandDepartment of Biomaterials, AGH‐University of Science and Technology, A. Mickiewicz 30, 30-059 Krakow, PolandAcademy of Physical Education, Department of Physiotherapy, Section of Anatomy, 31-008 Krakow, PolandInstitute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, PolandCurrent restrictions on the use of antibiotics, associated with increases in bacterial resistance, require new solutions, including materials with antibacterial properties. In this study, copper alginate fibers obtained using the classic wet method were used to make nonwovens which were modified with arginine-glycine-aspartic acid (RGD) derivatives. Stable polysaccharide-peptide conjugates formed by coupling with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO<sup>−</sup>), and materials with physically embedded RGD derivatives, were obtained. The materials were found to be characterized by very high antibacterial activity against <i>S. aureus</i> and <i>K. pneumoniae</i>. Cytotoxicity studies confirmed that the materials are not cytotoxic. Copper alginate conjugates with RGD peptides have strong potential for use in regenerative medicine, due to their biocompatibility and innate antibacterial activity.https://www.mdpi.com/1996-1944/13/2/337copper alginatergd derivativespolysaccharide-peptide conjugatesantibacterial activitycytotoxicity |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Justyna Fraczyk Joanna Wasko Malgorzata Walczak Zbigniew J. Kaminski Dorota Puchowicz Irena Kaminska Maciej Bogun Marcin Kolasa Ewa Stodolak-Zych Anna Scislowska-Czarnecka Beata Kolesinska |
| spellingShingle |
Justyna Fraczyk Joanna Wasko Malgorzata Walczak Zbigniew J. Kaminski Dorota Puchowicz Irena Kaminska Maciej Bogun Marcin Kolasa Ewa Stodolak-Zych Anna Scislowska-Czarnecka Beata Kolesinska Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine Materials copper alginate rgd derivatives polysaccharide-peptide conjugates antibacterial activity cytotoxicity |
| author_facet |
Justyna Fraczyk Joanna Wasko Malgorzata Walczak Zbigniew J. Kaminski Dorota Puchowicz Irena Kaminska Maciej Bogun Marcin Kolasa Ewa Stodolak-Zych Anna Scislowska-Czarnecka Beata Kolesinska |
| author_sort |
Justyna Fraczyk |
| title |
Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine |
| title_short |
Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine |
| title_full |
Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine |
| title_fullStr |
Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine |
| title_full_unstemmed |
Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine |
| title_sort |
conjugates of copper alginate with arginine-glycine-aspartic acid (rgd) for potential use in regenerative medicine |
| publisher |
MDPI AG |
| series |
Materials |
| issn |
1996-1944 |
| publishDate |
2020-01-01 |
| description |
Current restrictions on the use of antibiotics, associated with increases in bacterial resistance, require new solutions, including materials with antibacterial properties. In this study, copper alginate fibers obtained using the classic wet method were used to make nonwovens which were modified with arginine-glycine-aspartic acid (RGD) derivatives. Stable polysaccharide-peptide conjugates formed by coupling with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO<sup>−</sup>), and materials with physically embedded RGD derivatives, were obtained. The materials were found to be characterized by very high antibacterial activity against <i>S. aureus</i> and <i>K. pneumoniae</i>. Cytotoxicity studies confirmed that the materials are not cytotoxic. Copper alginate conjugates with RGD peptides have strong potential for use in regenerative medicine, due to their biocompatibility and innate antibacterial activity. |
| topic |
copper alginate rgd derivatives polysaccharide-peptide conjugates antibacterial activity cytotoxicity |
| url |
https://www.mdpi.com/1996-1944/13/2/337 |
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