In utero intraocular AAV injection for early gene expression in the developing rodent retina

In utero intraocular AAV injection for early gene expression in the developing rodent retina

Summary: The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the...

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Main Authors: Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Hisashi Umemori
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
developmental biology
gene expression
model organisms
molecular biology
neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004494
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spelling doaj-8a9a6a12c47c404ea6c783c7e9360ebb2021-09-19T05:00:54ZengElsevierSTAR Protocols2666-16672021-09-0123100742In utero intraocular AAV injection for early gene expression in the developing rodent retinaMasahiro Yasuda0Sivapratha Nagappan-Chettiar1Hisashi Umemori2Department of Neurology, F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USADepartment of Neurology, F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USADepartment of Neurology, F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the expression of transgenes early in the developing retina are essential to study visual system development. Here, we describe a protocol to express genes-of-interest in the developing mouse retina via in utero intraocular adeno-associated virus injections.For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721004494developmental biologygene expressionmodel organismsmolecular biologyneuroscience
collection DOAJ
language English
format Article
sources DOAJ
author Masahiro Yasuda
Sivapratha Nagappan-Chettiar
Hisashi Umemori
spellingShingle Masahiro Yasuda
Sivapratha Nagappan-Chettiar
Hisashi Umemori
In utero intraocular AAV injection for early gene expression in the developing rodent retina
STAR Protocols
developmental biology
gene expression
model organisms
molecular biology
neuroscience
author_facet Masahiro Yasuda
Sivapratha Nagappan-Chettiar
Hisashi Umemori
author_sort Masahiro Yasuda
title In utero intraocular AAV injection for early gene expression in the developing rodent retina
title_short In utero intraocular AAV injection for early gene expression in the developing rodent retina
title_full In utero intraocular AAV injection for early gene expression in the developing rodent retina
title_fullStr In utero intraocular AAV injection for early gene expression in the developing rodent retina
title_full_unstemmed In utero intraocular AAV injection for early gene expression in the developing rodent retina
title_sort in utero intraocular aav injection for early gene expression in the developing rodent retina
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-09-01
description Summary: The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the expression of transgenes early in the developing retina are essential to study visual system development. Here, we describe a protocol to express genes-of-interest in the developing mouse retina via in utero intraocular adeno-associated virus injections.For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).
topic developmental biology
gene expression
model organisms
molecular biology
neuroscience
url http://www.sciencedirect.com/science/article/pii/S2666166721004494
work_keys_str_mv AT masahiroyasuda inuterointraocularaavinjectionforearlygeneexpressioninthedevelopingrodentretina
AT sivaprathanagappanchettiar inuterointraocularaavinjectionforearlygeneexpressioninthedevelopingrodentretina
AT hisashiumemori inuterointraocularaavinjectionforearlygeneexpressioninthedevelopingrodentretina
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