Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016
Introduction: Urinary tract infection (UTI) caused by E coli is one of the most common diseases in community. Colonization of E. coli and its attachment to the uroepithelium are mediated by adhesions such as P, Fim and S fimbriae. The present study was aimed to evaluate the prevale...
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Shahid Sadoughi University of Medical Sciences
2017-02-01
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doaj-8aa23bac3c454c03b76410f636c930812020-11-25T00:30:23ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332017-02-012411913923Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016mohsen mirzaee01 Islamic azad university Introduction: Urinary tract infection (UTI) caused by E coli is one of the most common diseases in community. Colonization of E. coli and its attachment to the uroepithelium are mediated by adhesions such as P, Fim and S fimbriae. The present study was aimed to evaluate the prevalence of fimbrial virulence genes in Escherichia coli (E. coli) isolates from the patients with urinary tract infection in Tehran, Boroujerd and Sanandaj cities, Iran. Methods: In this descriptive cross sectional study, 150 clinical isolates of uropathogenic E.coli were collected from the patients with urinary tract infection. All bacterial isolates were identified by standard biochemical laboratory methods; the fim, pap and sfa genes were detected using the PCR and Multiplex-PCR methods. Results: One hundred forty-five (96.66%) isolates were positive for fim gene, one hundred forty (93.33%) isolates were positive for pap gene and seven (4.66) isolates were positive for sfa gene. Whole of the isolates were possessed at least one of the three virulence genes. Six (4%) isolates were positive for all genes. Conclusion: The findings of this study showed the high frequency of fim and P fimbriae among uropathogenic E.coli isolates from the patients with urinary tract infection. Because of the higher prevalence of UTI in the presence of these genes, detection of the genes in urine samples may help in more suspicious and rapid management of UTI.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2609-1&slc_lang=en&sid=1 Escherichia coli pap fim sfa genes Urinary tract infection |
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mohsen mirzaee |
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mohsen mirzaee Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016 Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Escherichia coli pap fim sfa genes Urinary tract infection |
author_facet |
mohsen mirzaee |
author_sort |
mohsen mirzaee |
title |
Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016 |
title_short |
Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016 |
title_full |
Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016 |
title_fullStr |
Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016 |
title_full_unstemmed |
Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016 |
title_sort |
frequency of fimbrial virulence genes (fim, pap, sfa) in escherichia coli isolated from the patients with urinary tract infections from selective hospitals of tehran, boroujerd and sanandej city in 2015-2016 |
publisher |
Shahid Sadoughi University of Medical Sciences |
series |
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
issn |
2228-5741 2228-5733 |
publishDate |
2017-02-01 |
description |
Introduction: Urinary tract infection (UTI) caused by E coli is one of the most common diseases in community. Colonization of E. coli and its attachment to the uroepithelium are mediated by adhesions such as P, Fim and S fimbriae. The present study was aimed to evaluate the prevalence of fimbrial virulence genes in Escherichia coli (E. coli) isolates from the patients with urinary tract infection in Tehran, Boroujerd and Sanandaj cities, Iran.
Methods: In this descriptive cross sectional study, 150 clinical isolates of uropathogenic E.coli were collected from the patients with urinary tract infection. All bacterial isolates were identified by standard biochemical laboratory methods; the fim, pap and sfa genes were detected using the PCR and Multiplex-PCR methods.
Results: One hundred forty-five (96.66%) isolates were positive for fim gene, one hundred forty (93.33%) isolates were positive for pap gene and seven (4.66) isolates were positive for sfa gene. Whole of the isolates were possessed at least one of the three virulence genes. Six (4%) isolates were positive for all genes.
Conclusion: The findings of this study showed the high frequency of fim and P fimbriae among uropathogenic E.coli isolates from the patients with urinary tract infection. Because of the higher prevalence of UTI in the presence of these genes, detection of the genes in urine samples may help in more suspicious and rapid management of UTI. |
topic |
Escherichia coli pap fim sfa genes Urinary tract infection |
url |
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2609-1&slc_lang=en&sid=1 |
work_keys_str_mv |
AT mohsenmirzaee frequencyoffimbrialvirulencegenesfimpapsfainescherichiacoliisolatedfromthepatientswithurinarytractinfectionsfromselectivehospitalsoftehranboroujerdandsanandejcityin20152016 |
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