Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells
<p>Abstract</p> <p>Background</p> <p>α-Mangostin (α-MG) is a main constituent of the fruit hull of the mangosteen. Previous studies have shown that α-MG has pharmacological activities such as antioxidant, antitumor, anti-inflammatory, antiallergic, antibacterial, antifu...
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doaj-8aaa75a120dc470f867d4eecddfcf4e52020-11-24T20:51:43ZengBMCChinese Medicine1749-85462012-08-01711910.1186/1749-8546-7-19Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cellsLiu Szu-HsiuLee Lain-TzeHu Nai-YunHuange Kuo-KueiShih Ying-ChuMunekazu IinumaLi Jen-MingChou Ting-YuWang Wei-HsinChen Ting-Shou<p>Abstract</p> <p>Background</p> <p>α-Mangostin (α-MG) is a main constituent of the fruit hull of the mangosteen. Previous studies have shown that α-MG has pharmacological activities such as antioxidant, antitumor, anti-inflammatory, antiallergic, antibacterial, antifungal and antiviral effects. This study aims to investigate the anti-inflammatory molecular action of α-MG on gene expression profiles.</p> <p>Methods</p> <p>U937 and EL4 cells were treated with different concentrations of α-MG in the presence of 0.1 ng/mL lipopolysaccharide (LPS) for 4 h. The anti-inflammatory effects of α-MG were measured by the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-4 in cell culture media, which were determined with enzyme-linked immunosorbent assay kits. The gene expression profiles of all samples were analyzed with a whole human genome microarray, Illumina BeadChip WG-6 version 3, containing 48804 probes. The protein levels were determined by Western blotting analyses.</p> <p>Results</p> <p>α-MG decreased the LPS induction of the inflammatory cytokines TNF-α (<it>P</it> = 0.038) and IL-4 (<it>P</it> = 0.04). α-MG decreased the gene expressions in oncostatin M signaling <it>via</it> mitogen-activated protein kinase (MAPK) pathways, including extracellular signal-regulated kinases (<it>P</it> = 0.016), c-Jun N-terminal kinase (<it>P</it> = 0.01) , and p38 (<it>P</it> = 0.008). α-MG treatment of U937 cells reduced the phosphorylation of MAPK kinase 3 / MAPK kinase 6 (<it>P</it> = 0.0441), MAPK-activated protein kinase-2 (<it>P</it> = 0.0453), signal transducers and activators of transcription-1 (STAT1) (<it>P</it> = 0.0012), c-Fos (<it>P</it> = 0.04), c-Jun (<it>P</it> = 0.019) and Ets-like molecule 1 (Elk-1) (<it>P</it> = 0.038).</p> <p>Conclusion</p> <p>This study demonstrates that α-MG attenuates LPS-mediated activation of MAPK, STAT1, c-Fos, c-Jun and EIK-1, inhibiting TNF-α and IL-4 production in U937 cells.</p> http://www.cmjournal.org/content/7/1/19 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liu Szu-Hsiu Lee Lain-Tze Hu Nai-Yun Huange Kuo-Kuei Shih Ying-Chu Munekazu Iinuma Li Jen-Ming Chou Ting-Yu Wang Wei-Hsin Chen Ting-Shou |
spellingShingle |
Liu Szu-Hsiu Lee Lain-Tze Hu Nai-Yun Huange Kuo-Kuei Shih Ying-Chu Munekazu Iinuma Li Jen-Ming Chou Ting-Yu Wang Wei-Hsin Chen Ting-Shou Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells Chinese Medicine |
author_facet |
Liu Szu-Hsiu Lee Lain-Tze Hu Nai-Yun Huange Kuo-Kuei Shih Ying-Chu Munekazu Iinuma Li Jen-Ming Chou Ting-Yu Wang Wei-Hsin Chen Ting-Shou |
author_sort |
Liu Szu-Hsiu |
title |
Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells |
title_short |
Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells |
title_full |
Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells |
title_fullStr |
Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells |
title_full_unstemmed |
Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells |
title_sort |
effects of alpha-mangostin on the expression of anti-inflammatory genes in u937 cells |
publisher |
BMC |
series |
Chinese Medicine |
issn |
1749-8546 |
publishDate |
2012-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>α-Mangostin (α-MG) is a main constituent of the fruit hull of the mangosteen. Previous studies have shown that α-MG has pharmacological activities such as antioxidant, antitumor, anti-inflammatory, antiallergic, antibacterial, antifungal and antiviral effects. This study aims to investigate the anti-inflammatory molecular action of α-MG on gene expression profiles.</p> <p>Methods</p> <p>U937 and EL4 cells were treated with different concentrations of α-MG in the presence of 0.1 ng/mL lipopolysaccharide (LPS) for 4 h. The anti-inflammatory effects of α-MG were measured by the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-4 in cell culture media, which were determined with enzyme-linked immunosorbent assay kits. The gene expression profiles of all samples were analyzed with a whole human genome microarray, Illumina BeadChip WG-6 version 3, containing 48804 probes. The protein levels were determined by Western blotting analyses.</p> <p>Results</p> <p>α-MG decreased the LPS induction of the inflammatory cytokines TNF-α (<it>P</it> = 0.038) and IL-4 (<it>P</it> = 0.04). α-MG decreased the gene expressions in oncostatin M signaling <it>via</it> mitogen-activated protein kinase (MAPK) pathways, including extracellular signal-regulated kinases (<it>P</it> = 0.016), c-Jun N-terminal kinase (<it>P</it> = 0.01) , and p38 (<it>P</it> = 0.008). α-MG treatment of U937 cells reduced the phosphorylation of MAPK kinase 3 / MAPK kinase 6 (<it>P</it> = 0.0441), MAPK-activated protein kinase-2 (<it>P</it> = 0.0453), signal transducers and activators of transcription-1 (STAT1) (<it>P</it> = 0.0012), c-Fos (<it>P</it> = 0.04), c-Jun (<it>P</it> = 0.019) and Ets-like molecule 1 (Elk-1) (<it>P</it> = 0.038).</p> <p>Conclusion</p> <p>This study demonstrates that α-MG attenuates LPS-mediated activation of MAPK, STAT1, c-Fos, c-Jun and EIK-1, inhibiting TNF-α and IL-4 production in U937 cells.</p> |
url |
http://www.cmjournal.org/content/7/1/19 |
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