Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937
Black walnut (Juglans nigra L.) is an excellent source of health-promoting compounds. Consumption of black walnuts has been linked to many health benefits (e.g., anti-inflammatory) stemming from its phytochemical composition and medicinal properties, but these effects have not been systematically st...
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doaj-8ac07cdb881f47eab0def19b0f2082db2020-11-25T00:44:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-09-011010.3389/fphar.2019.01059460177Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937Khanh-Van Ho0Khanh-Van Ho1Kathy L. Schreiber2Danh C. Vu3Susan M. Rottinghaus4Daniel E. Jackson5Charles R. Brown6Zhentian Lei7Zhentian Lei8Lloyd W. Sumner9Lloyd W. Sumner10Mark V. Coggeshall11Chung-Ho Lin12Center for Agroforestry, School of Natural Resources, University of Missouri, Columbia, MO, United StatesDepartment of Food Technology, Can Tho University, Can Tho, VietnamCell and Immunobiology Core, University of Missouri, Columbia, MO, United StatesCenter for Agroforestry, School of Natural Resources, University of Missouri, Columbia, MO, United StatesCell and Immunobiology Core, University of Missouri, Columbia, MO, United StatesCell and Immunobiology Core, University of Missouri, Columbia, MO, United StatesDepartment of Veterinary Pathobiology, University of Missouri, Columbia, MO, United StatesMetabolomics Center, University of Missouri, Columbia, MO, United StatesDepartment of Biochemistry, Bond Life Sciences Center, University of Missouri, Columbia, MO, United StatesMetabolomics Center, University of Missouri, Columbia, MO, United StatesDepartment of Biochemistry, Bond Life Sciences Center, University of Missouri, Columbia, MO, United StatesUnited States Northern Research Station, USDA-Forest Service, West Lafayette, IN, United StatesCenter for Agroforestry, School of Natural Resources, University of Missouri, Columbia, MO, United StatesBlack walnut (Juglans nigra L.) is an excellent source of health-promoting compounds. Consumption of black walnuts has been linked to many health benefits (e.g., anti-inflammatory) stemming from its phytochemical composition and medicinal properties, but these effects have not been systematically studied or characterized. In this study, potential anti-inflammatory compounds found in kernel extracts of 10 black walnut cultivars were putatively identified using a metabolomic profiling analysis, revealing differences in potential anti-inflammatory capacities among examined cultivars. Five cultivars were examined for activities in the human promonocytic cell line U-937 by evaluating the effects of the extracts on the expression of six human inflammatory cytokines/chemokines using a bead-based, flow cytometric multiplex assay. The methanolic extracts of these cultivars were added at four concentrations (0.1, 0.3, 1, and 10 mg/ml) either before and after the addition of lipopolysaccharide (LPS) to human U-937 cells to examine their effect on cytokine production. Results from cytotoxicity and viability assays revealed that the kernel extracts had no toxic effect on the U-937 cells. Of the 13 cytokines [interleukin (IL)-1β, tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein (MCP)-1, IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, IL-23, IL-33, interferon (IFN)-α, IFN-γ] measured, only six were detected under the culture conditions. The production of the six detected cytokines by phorbol 12-myristate 13-acetate (PMA)-differentiated, LPS-stimulated U-937 was significantly inhibited by the kernel extracts from two cultivars Surprise and Sparrow when the extracts were added before the addition of LPS. Other cultivars (Daniel, Mystry, and Sparks) showed weak or no significant effects on cytokine production. In contrast, no inhibitory effect was observed on the production of cytokines by PMA-differentiated, LPS-stimulated U-937 when the kernel extracts were added after the addition of LPS. The findings suggest that the extracts from certain black walnut cultivars, such as Sparrow and Surprise, are promising biological candidates for potentially decreasing the severity of inflammatory disease.https://www.frontiersin.org/article/10.3389/fphar.2019.01059/fullblack walnutJuglans nigrametabolomic profilingcytokine suppressionpotential anti-inflammatory compounds |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khanh-Van Ho Khanh-Van Ho Kathy L. Schreiber Danh C. Vu Susan M. Rottinghaus Daniel E. Jackson Charles R. Brown Zhentian Lei Zhentian Lei Lloyd W. Sumner Lloyd W. Sumner Mark V. Coggeshall Chung-Ho Lin |
spellingShingle |
Khanh-Van Ho Khanh-Van Ho Kathy L. Schreiber Danh C. Vu Susan M. Rottinghaus Daniel E. Jackson Charles R. Brown Zhentian Lei Zhentian Lei Lloyd W. Sumner Lloyd W. Sumner Mark V. Coggeshall Chung-Ho Lin Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937 Frontiers in Pharmacology black walnut Juglans nigra metabolomic profiling cytokine suppression potential anti-inflammatory compounds |
author_facet |
Khanh-Van Ho Khanh-Van Ho Kathy L. Schreiber Danh C. Vu Susan M. Rottinghaus Daniel E. Jackson Charles R. Brown Zhentian Lei Zhentian Lei Lloyd W. Sumner Lloyd W. Sumner Mark V. Coggeshall Chung-Ho Lin |
author_sort |
Khanh-Van Ho |
title |
Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937 |
title_short |
Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937 |
title_full |
Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937 |
title_fullStr |
Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937 |
title_full_unstemmed |
Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937 |
title_sort |
black walnut (juglans nigra) extracts inhibit proinflammatory cytokine production from lipopolysaccharide-stimulated human promonocytic cell line u-937 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2019-09-01 |
description |
Black walnut (Juglans nigra L.) is an excellent source of health-promoting compounds. Consumption of black walnuts has been linked to many health benefits (e.g., anti-inflammatory) stemming from its phytochemical composition and medicinal properties, but these effects have not been systematically studied or characterized. In this study, potential anti-inflammatory compounds found in kernel extracts of 10 black walnut cultivars were putatively identified using a metabolomic profiling analysis, revealing differences in potential anti-inflammatory capacities among examined cultivars. Five cultivars were examined for activities in the human promonocytic cell line U-937 by evaluating the effects of the extracts on the expression of six human inflammatory cytokines/chemokines using a bead-based, flow cytometric multiplex assay. The methanolic extracts of these cultivars were added at four concentrations (0.1, 0.3, 1, and 10 mg/ml) either before and after the addition of lipopolysaccharide (LPS) to human U-937 cells to examine their effect on cytokine production. Results from cytotoxicity and viability assays revealed that the kernel extracts had no toxic effect on the U-937 cells. Of the 13 cytokines [interleukin (IL)-1β, tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein (MCP)-1, IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, IL-23, IL-33, interferon (IFN)-α, IFN-γ] measured, only six were detected under the culture conditions. The production of the six detected cytokines by phorbol 12-myristate 13-acetate (PMA)-differentiated, LPS-stimulated U-937 was significantly inhibited by the kernel extracts from two cultivars Surprise and Sparrow when the extracts were added before the addition of LPS. Other cultivars (Daniel, Mystry, and Sparks) showed weak or no significant effects on cytokine production. In contrast, no inhibitory effect was observed on the production of cytokines by PMA-differentiated, LPS-stimulated U-937 when the kernel extracts were added after the addition of LPS. The findings suggest that the extracts from certain black walnut cultivars, such as Sparrow and Surprise, are promising biological candidates for potentially decreasing the severity of inflammatory disease. |
topic |
black walnut Juglans nigra metabolomic profiling cytokine suppression potential anti-inflammatory compounds |
url |
https://www.frontiersin.org/article/10.3389/fphar.2019.01059/full |
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