ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis
Meiotic recombination ensures accurate homologous chromosome segregation during meiosis and generates novel allelic combinations among gametes. During meiosis, DNA double strand breaks (DSBs) are generated to facilitate recombination. To maintain genome integrity, meiotic DSBs must be repaired using...
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doaj-8ac7ad077a0945d5b2cd74bc18ab3d522020-11-25T03:55:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00839542043ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in ArabidopsisYuan Yao0Xiaojing Li1Wanli Chen2Hui Liu3Limin Mi4Ding Ren5Aowei Mo6Pingli Lu7School of Life Sciences, Fudan University, Shanghai, ChinaSchool of Life Sciences, Fudan University, Shanghai, ChinaSchool of Life Sciences, Fudan University, Shanghai, ChinaSchool of Life Sciences, Fudan University, Shanghai, ChinaSchool of Life Sciences, Fudan University, Shanghai, ChinaSchool of Life Sciences, Fudan University, Shanghai, ChinaSchool of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, ChinaMeiotic recombination ensures accurate homologous chromosome segregation during meiosis and generates novel allelic combinations among gametes. During meiosis, DNA double strand breaks (DSBs) are generated to facilitate recombination. To maintain genome integrity, meiotic DSBs must be repaired using appropriate DNA templates. Although the DNA damage response protein kinase Ataxia-telangiectasia mutated (ATM) has been shown to be involved in meiotic recombination in Arabidopsis, its mechanistic role is still unclear. In this study, we performed cytological analysis in Arabidopsis atm mutant, we show that there are fewer γH2AX foci, but more RAD51 and DMC1 foci on atm meiotic chromosomes. Furthermore, we observed an increase in meiotic Type I crossovers (COs) in atm. Our genetic analysis shows that the meiotic phenotype of atm rad51 double mutants is similar to the rad51 single mutant. Whereas, the atm dmc1 double mutant has a more severe chromosome fragmentation phenotype compared to both single mutants, suggesting that ATM functions in concert with RAD51, but in parallel to DMC1. Lastly, we show that atm asy1 double mutants also have more severe meiotic recombination defects. These data lead us to propose a model wherein ATM promotes RAD51-mediated meiotic DSB repair by inter-sister-chromatid (IS) recombination in Arabidopsis.https://www.frontiersin.org/article/10.3389/fpls.2020.00839/fullmeiosisrecombinationDSBsinter-sister chromatidsATMRAD51 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuan Yao Xiaojing Li Wanli Chen Hui Liu Limin Mi Ding Ren Aowei Mo Pingli Lu |
spellingShingle |
Yuan Yao Xiaojing Li Wanli Chen Hui Liu Limin Mi Ding Ren Aowei Mo Pingli Lu ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis Frontiers in Plant Science meiosis recombination DSBs inter-sister chromatids ATM RAD51 |
author_facet |
Yuan Yao Xiaojing Li Wanli Chen Hui Liu Limin Mi Ding Ren Aowei Mo Pingli Lu |
author_sort |
Yuan Yao |
title |
ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis |
title_short |
ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis |
title_full |
ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis |
title_fullStr |
ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis |
title_full_unstemmed |
ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis |
title_sort |
atm promotes rad51-mediated meiotic dsb repair by inter-sister-chromatid recombination in arabidopsis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-06-01 |
description |
Meiotic recombination ensures accurate homologous chromosome segregation during meiosis and generates novel allelic combinations among gametes. During meiosis, DNA double strand breaks (DSBs) are generated to facilitate recombination. To maintain genome integrity, meiotic DSBs must be repaired using appropriate DNA templates. Although the DNA damage response protein kinase Ataxia-telangiectasia mutated (ATM) has been shown to be involved in meiotic recombination in Arabidopsis, its mechanistic role is still unclear. In this study, we performed cytological analysis in Arabidopsis atm mutant, we show that there are fewer γH2AX foci, but more RAD51 and DMC1 foci on atm meiotic chromosomes. Furthermore, we observed an increase in meiotic Type I crossovers (COs) in atm. Our genetic analysis shows that the meiotic phenotype of atm rad51 double mutants is similar to the rad51 single mutant. Whereas, the atm dmc1 double mutant has a more severe chromosome fragmentation phenotype compared to both single mutants, suggesting that ATM functions in concert with RAD51, but in parallel to DMC1. Lastly, we show that atm asy1 double mutants also have more severe meiotic recombination defects. These data lead us to propose a model wherein ATM promotes RAD51-mediated meiotic DSB repair by inter-sister-chromatid (IS) recombination in Arabidopsis. |
topic |
meiosis recombination DSBs inter-sister chromatids ATM RAD51 |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.00839/full |
work_keys_str_mv |
AT yuanyao atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT xiaojingli atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT wanlichen atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT huiliu atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT liminmi atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT dingren atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT aoweimo atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis AT pinglilu atmpromotesrad51mediatedmeioticdsbrepairbyintersisterchromatidrecombinationinarabidopsis |
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