The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
Abstract This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optom...
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2021-07-01
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Online Access: | https://doi.org/10.1038/s41598-021-93078-y |
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doaj-8acecfae3a674fb4b832ae230530496f2021-07-11T11:29:42ZengNature Publishing GroupScientific Reports2045-23222021-07-0111111010.1038/s41598-021-93078-yThe evaluation of zebrafish cardiovascular and behavioral functions through microfluidicsSatishkumar Subendran0Yi-Chieh Wang1Yueh-Hsun Lu2Chia-Yuan Chen3Department of Mechanical Engineering, National Cheng Kung UniversityDepartment of Mechanical Engineering, National Cheng Kung UniversityDepartment of Radiology, Shuang-Ho Hospital, Taipei Medical UniversityDepartment of Mechanical Engineering, National Cheng Kung UniversityAbstract This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research.https://doi.org/10.1038/s41598-021-93078-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Satishkumar Subendran Yi-Chieh Wang Yueh-Hsun Lu Chia-Yuan Chen |
spellingShingle |
Satishkumar Subendran Yi-Chieh Wang Yueh-Hsun Lu Chia-Yuan Chen The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics Scientific Reports |
author_facet |
Satishkumar Subendran Yi-Chieh Wang Yueh-Hsun Lu Chia-Yuan Chen |
author_sort |
Satishkumar Subendran |
title |
The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics |
title_short |
The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics |
title_full |
The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics |
title_fullStr |
The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics |
title_full_unstemmed |
The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics |
title_sort |
evaluation of zebrafish cardiovascular and behavioral functions through microfluidics |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-07-01 |
description |
Abstract This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research. |
url |
https://doi.org/10.1038/s41598-021-93078-y |
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