Blood typing in positive DEA 1 dogs

• Main Authors: Suzana Claudia Spinola Santos, Mariane Melo Santos, Wellington Francisco Rodrigues, Roberto Meyer, Maria de Fátima Dias Costa
• Format: Article
• Language: English
• Published: Universidade de São Paulo 2020-03-01
• Series: Brazilian Journal of Veterinary Research and Animal Science
• Subjects: Canine transfusion medicine; Transfusion risk in dogs; Dog erythrocyte antigen 1; Flow cytometry
• Online Access: http://www.revistas.usp.br/bjvras/article/view/151444

Blood typing techniques have been improved to ensure greater safety for transfusion procedures. Typification for the DEA 1 antigen through flow cytometry should offer more reliability to routine immunohematology in donor and recipient dogs. Currently, the DEA 1 group is starting to be an autosomal dominant allelic system with the DEA 1 negative type and its variations of positivity. The present study investigated the DEA 1 antigen using the techniques of immunochromatography, hemagglutination and flow cytometry. Among the positive animals for the DEA 1 group, typified by flow cytometry, medium intensities of fluorescence were found, which are indicative of weak, moderate and strong antigenicity. This enabled the division of the DEA 1 group into weak positive, moderate positive and strong positive. The blood typing techniques for the DEA 1 group by flow cytometry, agglutination and immunochromatography had positive (Spearman r=0.70) and statistically significant (p>0.0001) correlations.