Size scaling of nucleolus in Caenorhabditis elegans embryos

Size scaling of nucleolus in Caenorhabditis elegans embryos

Nucleolus is viewed as a plurifunctional center in the cell, tightly linked to ribosome biosynthesis. As a non-membranous structure, how the size of nucleolus is determined is a long outstanding question, and the possibility of “direct size scaling to the nucleus” was raised by genetic studies in fi...

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Main Authors: Tian-Hsiang Ma, Po-Hsiang Chen, Bertrand Chin-Ming Tan, Szecheng J. Lo
Format: Article
Language:English
Published: Elsevier 2018-10-01
Series:Biomedical Journal
Online Access:http://www.sciencedirect.com/science/article/pii/S2319417018302762
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spelling doaj-8af717b003234214b65bac774c1e65512021-02-02T07:33:06ZengElsevierBiomedical Journal2319-41702018-10-01415333336Size scaling of nucleolus in Caenorhabditis elegans embryosTian-Hsiang Ma0Po-Hsiang Chen1Bertrand Chin-Ming Tan2Szecheng J. Lo3Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Neurosurgery, Chang Gung Memorial Hospital at Linlou, Taoyuan, Taiwan; Research Center for Emerging Viral Infections, Chang Gung Memorial Hospital at Linlou, Taoyuan, Taiwan; Corresponding author. Department of Biomedical Sciences, College of Medicine, Chang Gung University, 259, Wenhua 1st Rd., Gueishan, Taoyuan 333, Taiwan.Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Corresponding author. Department of Biomedical Sciences, College of Medicine, Chang Gung University, 259, Wenhua 1st Rd., Gueishan, Taoyuan 333, Taiwan.Nucleolus is viewed as a plurifunctional center in the cell, tightly linked to ribosome biosynthesis. As a non-membranous structure, how the size of nucleolus is determined is a long outstanding question, and the possibility of “direct size scaling to the nucleus” was raised by genetic studies in fission yeast. Here, we used the model organism Caenorhabditis elegans to test this hypothesis in multi-cellular organisms. We depleted ani-2, ima-3, or C27D9.1 by RNAi feeding, which altered embryo sizes to different extents in ncl-1 mutant worms. DIC imaging provided evidence that in size-altering embryo nucleolar size decreases in small cells and increases in large cells. Furthermore, analyses of nucleolar size in four blastomeres (ABa, ABp, EMS, and P2) within the same embryo of ncl-1 mutants consistently demonstrated the correspondence between cell and nucleolar sizes – the small cells (EMS and P2) have smaller nucleoli in comparison to the large cells (ABa). Keywords: Nucleolar size, Embryonic size, ani-2, ima-3, C27D9.1http://www.sciencedirect.com/science/article/pii/S2319417018302762
collection DOAJ
language English
format Article
sources DOAJ
author Tian-Hsiang Ma
Po-Hsiang Chen
Bertrand Chin-Ming Tan
Szecheng J. Lo
spellingShingle Tian-Hsiang Ma
Po-Hsiang Chen
Bertrand Chin-Ming Tan
Szecheng J. Lo
Size scaling of nucleolus in Caenorhabditis elegans embryos
Biomedical Journal
author_facet Tian-Hsiang Ma
Po-Hsiang Chen
Bertrand Chin-Ming Tan
Szecheng J. Lo
author_sort Tian-Hsiang Ma
title Size scaling of nucleolus in Caenorhabditis elegans embryos
title_short Size scaling of nucleolus in Caenorhabditis elegans embryos
title_full Size scaling of nucleolus in Caenorhabditis elegans embryos
title_fullStr Size scaling of nucleolus in Caenorhabditis elegans embryos
title_full_unstemmed Size scaling of nucleolus in Caenorhabditis elegans embryos
title_sort size scaling of nucleolus in caenorhabditis elegans embryos
publisher Elsevier
series Biomedical Journal
issn 2319-4170
publishDate 2018-10-01
description Nucleolus is viewed as a plurifunctional center in the cell, tightly linked to ribosome biosynthesis. As a non-membranous structure, how the size of nucleolus is determined is a long outstanding question, and the possibility of “direct size scaling to the nucleus” was raised by genetic studies in fission yeast. Here, we used the model organism Caenorhabditis elegans to test this hypothesis in multi-cellular organisms. We depleted ani-2, ima-3, or C27D9.1 by RNAi feeding, which altered embryo sizes to different extents in ncl-1 mutant worms. DIC imaging provided evidence that in size-altering embryo nucleolar size decreases in small cells and increases in large cells. Furthermore, analyses of nucleolar size in four blastomeres (ABa, ABp, EMS, and P2) within the same embryo of ncl-1 mutants consistently demonstrated the correspondence between cell and nucleolar sizes – the small cells (EMS and P2) have smaller nucleoli in comparison to the large cells (ABa). Keywords: Nucleolar size, Embryonic size, ani-2, ima-3, C27D9.1
url http://www.sciencedirect.com/science/article/pii/S2319417018302762
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AT pohsiangchen sizescalingofnucleolusincaenorhabditiselegansembryos
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