Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine
Bacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes. BAC cloning can also be useful for capturing representative genomes that comprise a viral stock or mixture. The Towne live attenuated cytomegalovirus vaccine was developed in the 1970...
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doaj-8afff70085ec42dea3b6c4aec94132ec2020-11-24T21:50:57ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512012-01-01201210.1155/2012/428498428498Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus VaccineXiaohong Cui0Stuart P. Adler1Andrew J. Davison2Larry Smith3EL-Sayed E. Habib4Michael A. McVoy5Department of Pediatrics, Medical College of Virginia Campus of Virginia Commonwealth University, 1101 E. Marshall Street, P.O. Box 980163, Richmond, VA 23298-0163, USADepartment of Pediatrics, Medical College of Virginia Campus of Virginia Commonwealth University, 1101 E. Marshall Street, P.O. Box 980163, Richmond, VA 23298-0163, USAMRC-University of Glasgow Centre for Virus Research, 8 Church Street, Glasgow G11 5JR, UKDepartment of Vaccine Research, Vical Incorporated, 10390 Pacific Center Court, San Diego, CA 92121, USADepartment of Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptDepartment of Pediatrics, Medical College of Virginia Campus of Virginia Commonwealth University, 1101 E. Marshall Street, P.O. Box 980163, Richmond, VA 23298-0163, USABacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes. BAC cloning can also be useful for capturing representative genomes that comprise a viral stock or mixture. The Towne live attenuated cytomegalovirus vaccine was developed in the 1970s by serial passage in cultured fibroblasts. Although its safety, immunogenicity, and efficacy have been evaluated in nearly a thousand human subjects, the vaccine itself has been little studied. Instead, genetic composition and in vitro growth properties have been inferred from studies of laboratory stocks that may not always accurately represent the viruses that comprise the vaccine. Here we describe the use of BAC cloning to define the genotypic and phenotypic properties of viruses from the Towne vaccine. Given the extensive safety history of the Towne vaccine, these BACs provide a logical starting point for the development of next-generation rationally engineered cytomegalovirus vaccines.http://dx.doi.org/10.1155/2012/428498 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaohong Cui Stuart P. Adler Andrew J. Davison Larry Smith EL-Sayed E. Habib Michael A. McVoy |
spellingShingle |
Xiaohong Cui Stuart P. Adler Andrew J. Davison Larry Smith EL-Sayed E. Habib Michael A. McVoy Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine Journal of Biomedicine and Biotechnology |
author_facet |
Xiaohong Cui Stuart P. Adler Andrew J. Davison Larry Smith EL-Sayed E. Habib Michael A. McVoy |
author_sort |
Xiaohong Cui |
title |
Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine |
title_short |
Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine |
title_full |
Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine |
title_fullStr |
Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine |
title_full_unstemmed |
Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine |
title_sort |
bacterial artificial chromosome clones of viruses comprising the towne cytomegalovirus vaccine |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2012-01-01 |
description |
Bacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes. BAC cloning can also be useful for capturing representative genomes that comprise a viral stock or mixture. The Towne live attenuated cytomegalovirus vaccine was developed in the 1970s by serial passage in cultured fibroblasts. Although its safety, immunogenicity, and efficacy have been evaluated in nearly a thousand human subjects, the vaccine itself has been little studied. Instead, genetic composition and in vitro growth properties have been inferred from studies of laboratory stocks that may not always accurately represent the viruses that comprise the vaccine. Here we describe the use of BAC cloning to define the genotypic and phenotypic properties of viruses from the Towne vaccine. Given the extensive safety history of the Towne vaccine, these BACs provide a logical starting point for the development of next-generation rationally engineered cytomegalovirus vaccines. |
url |
http://dx.doi.org/10.1155/2012/428498 |
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