Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran
Background: Infection with Ornithobilharzia turkestanicum has been reported in a wide range of animals worldwide. This study was undertaken to assess the util-ity of polymerase chain reaction (PCR), for detecting the infection with O. turke-stanicum larvae stages in Lymnaea gedrosiana. Metho...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2013-12-01
|
Series: | Iranian Journal of Parasitology |
Subjects: | |
Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/445 |
id |
doaj-8b0c7a14c2a946a187416f9bcdd6972a |
---|---|
record_format |
Article |
spelling |
doaj-8b0c7a14c2a946a187416f9bcdd6972a2021-03-02T09:01:47ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-12-0184424Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern IranMohammad Yakhchali0Seyyed Yaser Mirrajei1Reza Malekzadeh-Viayeh2Dept. of Pathobiology, Parasitology Division, Faculty of Veterinary Medicine, Nazlu campus, Urmia University, Urmia, Iran.Faculty of Veterinary Medicine, Nazlu campus, Urmia University, Urmia, Iran.Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, Iran. Background: Infection with Ornithobilharzia turkestanicum has been reported in a wide range of animals worldwide. This study was undertaken to assess the util-ity of polymerase chain reaction (PCR), for detecting the infection with O. turke-stanicum larvae stages in Lymnaea gedrosiana. Methods: A total of 6,759 Lymnaeidae snails were collected from six aquatic habitats in West Azarbaijan, northwest Iran. Of these, the snails of L. gedrosiana were identified. To detect infected L. gedrosiana with the larval stages of O. turke-stanicum, they were subjected for cercarial shedding and molecular examinations. The genomic DNA was extracted and PCR was performed to specifically ampli-fy a fragment of the nuclear 28SrRNA gene of O. turkestanicum. Results: Of all collected snails, 5.4% (365/6,759) were the snails of L. gedrosiana. The cercarial shedding method revealed that 23.56% (86/365) of the snails were infected. The PCR patterns confirmed that 28.77% (105/365) snails of L. gedrosiana were infected with larval stages of O. turkestanicum. The infected snails were observed in five studied sites. The highest infection rate (66.66%, 20/30) was recorded in the snails of Ghargologh in the northern part. Only 35.24% (37/105) of the infected snails were from the plain areas, whereas the remaining existed in high altitudes. Conclusion: It was concluded PCR method could be an efficient and fast method for uncovering the actual rate of infection with larval stages of O. turke-stanicum in the snails of L. gedrosiana. This method can be also useful for the do-mestic animals and public health management programs in the country. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/44528SrRNA geneIranLymnaea gedrosianaOrnithobilharzia turkestanicumPCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Yakhchali Seyyed Yaser Mirrajei Reza Malekzadeh-Viayeh |
spellingShingle |
Mohammad Yakhchali Seyyed Yaser Mirrajei Reza Malekzadeh-Viayeh Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran Iranian Journal of Parasitology 28SrRNA gene Iran Lymnaea gedrosiana Ornithobilharzia turkestanicum PCR |
author_facet |
Mohammad Yakhchali Seyyed Yaser Mirrajei Reza Malekzadeh-Viayeh |
author_sort |
Mohammad Yakhchali |
title |
Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran |
title_short |
Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran |
title_full |
Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran |
title_fullStr |
Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran |
title_full_unstemmed |
Detection of Infection with Larval Stages of Ornithobilharzia turkestanicum using PCR in Field-Collected Snails of Lymnaea gedrosiana from Northwestern Iran |
title_sort |
detection of infection with larval stages of ornithobilharzia turkestanicum using pcr in field-collected snails of lymnaea gedrosiana from northwestern iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2013-12-01 |
description |
Background: Infection with Ornithobilharzia turkestanicum has been reported in a wide range of animals worldwide. This study was undertaken to assess the util-ity of polymerase chain reaction (PCR), for detecting the infection with O. turke-stanicum larvae stages in Lymnaea gedrosiana.
Methods: A total of 6,759 Lymnaeidae snails were collected from six aquatic habitats in West Azarbaijan, northwest Iran. Of these, the snails of L. gedrosiana were identified. To detect infected L. gedrosiana with the larval stages of O. turke-stanicum, they were subjected for cercarial shedding and molecular examinations. The genomic DNA was extracted and PCR was performed to specifically ampli-fy a fragment of the nuclear 28SrRNA gene of O. turkestanicum.
Results: Of all collected snails, 5.4% (365/6,759) were the snails of L. gedrosiana. The cercarial shedding method revealed that 23.56% (86/365) of the snails were infected. The PCR patterns confirmed that 28.77% (105/365) snails of L. gedrosiana were infected with larval stages of O. turkestanicum. The infected snails were observed in five studied sites. The highest infection rate (66.66%, 20/30) was recorded in the snails of Ghargologh in the northern part. Only 35.24% (37/105) of the infected snails were from the plain areas, whereas the remaining existed in high altitudes.
Conclusion: It was concluded PCR method could be an efficient and fast method for uncovering the actual rate of infection with larval stages of O. turke-stanicum in the snails of L. gedrosiana. This method can be also useful for the do-mestic animals and public health management programs in the country.
|
topic |
28SrRNA gene Iran Lymnaea gedrosiana Ornithobilharzia turkestanicum PCR |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/445 |
work_keys_str_mv |
AT mohammadyakhchali detectionofinfectionwithlarvalstagesofornithobilharziaturkestanicumusingpcrinfieldcollectedsnailsoflymnaeagedrosianafromnorthwesterniran AT seyyedyasermirrajei detectionofinfectionwithlarvalstagesofornithobilharziaturkestanicumusingpcrinfieldcollectedsnailsoflymnaeagedrosianafromnorthwesterniran AT rezamalekzadehviayeh detectionofinfectionwithlarvalstagesofornithobilharziaturkestanicumusingpcrinfieldcollectedsnailsoflymnaeagedrosianafromnorthwesterniran |
_version_ |
1724240196024664064 |