Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling

Here we describe the effects of a controlled, 30 min, high-intensity cycling test on blood rheology and the metabolic profiles of red blood cells (RBCs) and plasma from well-trained males. RBCs demonstrated decreased deformability and trended toward increased generation of microparticles after the t...

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Main Authors: Travis Nemkov, Sarah C. Skinner, Elie Nader, Davide Stefanoni, Mélanie Robert, Francesca Cendali, Emeric Stauffer, Agnes Cibiel, Camille Boisson, Philippe Connes, Angelo D’Alessandro
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/22/2/896
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spelling doaj-8b0f8405c53740e1bc0c7cff5bb3873a2021-01-19T00:00:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-012289689610.3390/ijms22020896Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid RemodelingTravis Nemkov0Sarah C. Skinner1Elie Nader2Davide Stefanoni3Mélanie Robert4Francesca Cendali5Emeric Stauffer6Agnes Cibiel7Camille Boisson8Philippe Connes9Angelo D’Alessandro10Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USAUNC Blood Center, University of North Carolina, Chapel Hill, NC 27599, USAInter-University Laboratory of Biology of Motor Function EA7424, Vascular Biology and the Red Blood Cell Team, Claude Bernard University Lyon 1, University de Lyon 1, 69100 Villeurbanne, FranceDepartment of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USAInter-University Laboratory of Biology of Motor Function EA7424, Vascular Biology and the Red Blood Cell Team, Claude Bernard University Lyon 1, University de Lyon 1, 69100 Villeurbanne, FranceDepartment of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USAInter-University Laboratory of Biology of Motor Function EA7424, Vascular Biology and the Red Blood Cell Team, Claude Bernard University Lyon 1, University de Lyon 1, 69100 Villeurbanne, FranceErytech Pharma, 69008 Lyon, FranceInter-University Laboratory of Biology of Motor Function EA7424, Vascular Biology and the Red Blood Cell Team, Claude Bernard University Lyon 1, University de Lyon 1, 69100 Villeurbanne, FranceInter-University Laboratory of Biology of Motor Function EA7424, Vascular Biology and the Red Blood Cell Team, Claude Bernard University Lyon 1, University de Lyon 1, 69100 Villeurbanne, FranceDepartment of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USAHere we describe the effects of a controlled, 30 min, high-intensity cycling test on blood rheology and the metabolic profiles of red blood cells (RBCs) and plasma from well-trained males. RBCs demonstrated decreased deformability and trended toward increased generation of microparticles after the test. Meanwhile, metabolomics and lipidomics highlighted oxidative stress and activation of membrane lipid remodeling mechanisms in order to cope with altered properties of circulation resulting from physical exertion during the cycling test. Of note, intermediates from coenzyme A (CoA) synthesis for conjugation to fatty acyl chains, in parallel with reversible conversion of carnitine and acylcarnitines, emerged as metabolites that significantly correlate with RBC deformability and the generation of microparticles during exercise. Taken together, we propose that RBC membrane remodeling and repair plays an active role in the physiologic response to exercise by altering RBC properties.https://www.mdpi.com/1422-0067/22/2/896red blood celldeformabilitycyclingexercisemetabolomicslipidomics
collection DOAJ
language English
format Article
sources DOAJ
author Travis Nemkov
Sarah C. Skinner
Elie Nader
Davide Stefanoni
Mélanie Robert
Francesca Cendali
Emeric Stauffer
Agnes Cibiel
Camille Boisson
Philippe Connes
Angelo D’Alessandro
spellingShingle Travis Nemkov
Sarah C. Skinner
Elie Nader
Davide Stefanoni
Mélanie Robert
Francesca Cendali
Emeric Stauffer
Agnes Cibiel
Camille Boisson
Philippe Connes
Angelo D’Alessandro
Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling
International Journal of Molecular Sciences
red blood cell
deformability
cycling
exercise
metabolomics
lipidomics
author_facet Travis Nemkov
Sarah C. Skinner
Elie Nader
Davide Stefanoni
Mélanie Robert
Francesca Cendali
Emeric Stauffer
Agnes Cibiel
Camille Boisson
Philippe Connes
Angelo D’Alessandro
author_sort Travis Nemkov
title Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling
title_short Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling
title_full Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling
title_fullStr Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling
title_full_unstemmed Acute Cycling Exercise Induces Changes in Red Blood Cell Deformability and Membrane Lipid Remodeling
title_sort acute cycling exercise induces changes in red blood cell deformability and membrane lipid remodeling
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2021-01-01
description Here we describe the effects of a controlled, 30 min, high-intensity cycling test on blood rheology and the metabolic profiles of red blood cells (RBCs) and plasma from well-trained males. RBCs demonstrated decreased deformability and trended toward increased generation of microparticles after the test. Meanwhile, metabolomics and lipidomics highlighted oxidative stress and activation of membrane lipid remodeling mechanisms in order to cope with altered properties of circulation resulting from physical exertion during the cycling test. Of note, intermediates from coenzyme A (CoA) synthesis for conjugation to fatty acyl chains, in parallel with reversible conversion of carnitine and acylcarnitines, emerged as metabolites that significantly correlate with RBC deformability and the generation of microparticles during exercise. Taken together, we propose that RBC membrane remodeling and repair plays an active role in the physiologic response to exercise by altering RBC properties.
topic red blood cell
deformability
cycling
exercise
metabolomics
lipidomics
url https://www.mdpi.com/1422-0067/22/2/896
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