An alternative D. melanogaster 7SK snRNP
Abstract Background The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa. R...
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doaj-8b301efa1b3f4e5483927c30c0e051dc2021-09-05T11:36:17ZengBMCBMC Molecular and Cell Biology2661-88502021-08-0122111210.1186/s12860-021-00381-7An alternative D. melanogaster 7SK snRNPDuy Nguyen0Nicolas Buisine1Olivier Fayol2Annemieke A. Michels3Olivier Bensaude4David H. Price5Patricia Uguen6Université Paris-Saclay, INSERM, CNRS, Interactions cellulaires et physiopathologie hépatiqueMNHN, UMR CNRS 7221Université Paris-Saclay, INSERM, CNRS, Interactions cellulaires et physiopathologie hépatiqueIBENS Paris, UMR CNRS 8197; UA INSERM 1024IBENS Paris, UMR CNRS 8197; UA INSERM 1024Department of Biochemistry, University of IowaUniversité Paris-Saclay, INSERM, CNRS, Interactions cellulaires et physiopathologie hépatiqueAbstract Background The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa. Results Here, we describe a novel ncRNA sharing many features characteristic of 7SK RNAs, in D. melanogaster. We examined the structure of the corresponding gene and determined the expression profiles of the encoded RNA, called snRNA:7SK:94F, during development. It is probably produced from the transcription of a lncRNA which is processed into a mature snRNA. We also addressed its biological function and we show that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex, i.e. HEXIM, LARP7 and Cyclin T. This novel RNA is widely expressed across tissues. Conclusion We propose that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster.https://doi.org/10.1186/s12860-021-00381-77SK snRNADrosophilaP-TEFbLong non-coding RNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Duy Nguyen Nicolas Buisine Olivier Fayol Annemieke A. Michels Olivier Bensaude David H. Price Patricia Uguen |
spellingShingle |
Duy Nguyen Nicolas Buisine Olivier Fayol Annemieke A. Michels Olivier Bensaude David H. Price Patricia Uguen An alternative D. melanogaster 7SK snRNP BMC Molecular and Cell Biology 7SK snRNA Drosophila P-TEFb Long non-coding RNA |
author_facet |
Duy Nguyen Nicolas Buisine Olivier Fayol Annemieke A. Michels Olivier Bensaude David H. Price Patricia Uguen |
author_sort |
Duy Nguyen |
title |
An alternative D. melanogaster 7SK snRNP |
title_short |
An alternative D. melanogaster 7SK snRNP |
title_full |
An alternative D. melanogaster 7SK snRNP |
title_fullStr |
An alternative D. melanogaster 7SK snRNP |
title_full_unstemmed |
An alternative D. melanogaster 7SK snRNP |
title_sort |
alternative d. melanogaster 7sk snrnp |
publisher |
BMC |
series |
BMC Molecular and Cell Biology |
issn |
2661-8850 |
publishDate |
2021-08-01 |
description |
Abstract Background The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa. Results Here, we describe a novel ncRNA sharing many features characteristic of 7SK RNAs, in D. melanogaster. We examined the structure of the corresponding gene and determined the expression profiles of the encoded RNA, called snRNA:7SK:94F, during development. It is probably produced from the transcription of a lncRNA which is processed into a mature snRNA. We also addressed its biological function and we show that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex, i.e. HEXIM, LARP7 and Cyclin T. This novel RNA is widely expressed across tissues. Conclusion We propose that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster. |
topic |
7SK snRNA Drosophila P-TEFb Long non-coding RNA |
url |
https://doi.org/10.1186/s12860-021-00381-7 |
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