Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits
Purpose To evaluate the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptor, GDNF family receptor alpha subunit 1 (GFRα-1) in the pelvic (middle third) vagina and, particularly, in the paravaginal ganglia of nulliparous and primiparous rabbits. Methods Chinchilla-breed...
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Korean Continence Society
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doaj-8b33ce9d8df44b0197958e2136000cb42020-11-25T00:53:01ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312018-01-0122Suppl 1S233310.5213/inj.1834974.487706Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous RabbitsVerónica García-Villamar0Laura G. Hernández-Aragón1Jesús R. Chávez-Ríos2Arturo Ortega3Margarita Martínez-Gómez4Francisco Castelán5 Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México Depto. de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Unidad Foránea Tlaxcala, Universidad Nacional Autónoma de México, Tlaxcala, México Depto. de Toxicología, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, MéxicoPurpose To evaluate the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptor, GDNF family receptor alpha subunit 1 (GFRα-1) in the pelvic (middle third) vagina and, particularly, in the paravaginal ganglia of nulliparous and primiparous rabbits. Methods Chinchilla-breed female rabbits were used. Primiparas were killed on postpartum day 3 and nulliparas upon reaching a similar age. The vaginal tracts were processed for histological analyses or frozen for Western blot assays. We measured the ganglionic area, the Abercrombie-corrected number of paravaginal neurons, the cross-sectional area of the neuronal somata, and the number of satellite glial cells (SGCs) per neuron. The relative expression of both GDNF and GFRα-1 were assessed by Western blotting, and the immunostaining was semiquantitated. Unpaired two-tailed Student t -test or Wilcoxon test was used to identify statistically significant differences (P≤0.05) between the groups. Results Our findings demonstrated that the ganglionic area, neuronal soma size, Abercrombie-corrected number of neurons, and number of SGCs per neuron were similar in nulliparas and primiparas. The relative expression of both GDNF and GFRα-1 was similar. Immunostaining for both GDNF and GFRα-1 was observed in several vaginal layers, and no differences were detected regarding GDNF and GFRα-1 immunostaining between the 2 groups. In the paravaginal ganglia, the expression of GDNF was increased in neurons, while that of GFRα-1 was augmented in the SGCs of primiparous rabbits. Conclusions The present findings suggest an ongoing regenerative process related to the recovery of neuronal soma size in the paravaginal ganglia, in which GDNF and GFRα-1 could be involved in cross-talk between neurons and SGCs.http://www.einj.org/upload/pdf/inj-1834974-487.pdfPelvic gangliaNerve growth factorsNeuronal plasticityReproductionLower urogenital tract |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Verónica García-Villamar Laura G. Hernández-Aragón Jesús R. Chávez-Ríos Arturo Ortega Margarita Martínez-Gómez Francisco Castelán |
spellingShingle |
Verónica García-Villamar Laura G. Hernández-Aragón Jesús R. Chávez-Ríos Arturo Ortega Margarita Martínez-Gómez Francisco Castelán Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits International Neurourology Journal Pelvic ganglia Nerve growth factors Neuronal plasticity Reproduction Lower urogenital tract |
author_facet |
Verónica García-Villamar Laura G. Hernández-Aragón Jesús R. Chávez-Ríos Arturo Ortega Margarita Martínez-Gómez Francisco Castelán |
author_sort |
Verónica García-Villamar |
title |
Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits |
title_short |
Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits |
title_full |
Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits |
title_fullStr |
Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits |
title_full_unstemmed |
Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits |
title_sort |
expression of glial cell line-derived neurotrophic factor (gdnf) and the gdnf family receptor alpha subunit 1 in the paravaginal ganglia of nulliparous and primiparous rabbits |
publisher |
Korean Continence Society |
series |
International Neurourology Journal |
issn |
2093-4777 2093-6931 |
publishDate |
2018-01-01 |
description |
Purpose To evaluate the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptor, GDNF family receptor alpha subunit 1 (GFRα-1) in the pelvic (middle third) vagina and, particularly, in the paravaginal ganglia of nulliparous and primiparous rabbits. Methods Chinchilla-breed female rabbits were used. Primiparas were killed on postpartum day 3 and nulliparas upon reaching a similar age. The vaginal tracts were processed for histological analyses or frozen for Western blot assays. We measured the ganglionic area, the Abercrombie-corrected number of paravaginal neurons, the cross-sectional area of the neuronal somata, and the number of satellite glial cells (SGCs) per neuron. The relative expression of both GDNF and GFRα-1 were assessed by Western blotting, and the immunostaining was semiquantitated. Unpaired two-tailed Student t -test or Wilcoxon test was used to identify statistically significant differences (P≤0.05) between the groups. Results Our findings demonstrated that the ganglionic area, neuronal soma size, Abercrombie-corrected number of neurons, and number of SGCs per neuron were similar in nulliparas and primiparas. The relative expression of both GDNF and GFRα-1 was similar. Immunostaining for both GDNF and GFRα-1 was observed in several vaginal layers, and no differences were detected regarding GDNF and GFRα-1 immunostaining between the 2 groups. In the paravaginal ganglia, the expression of GDNF was increased in neurons, while that of GFRα-1 was augmented in the SGCs of primiparous rabbits. Conclusions The present findings suggest an ongoing regenerative process related to the recovery of neuronal soma size in the paravaginal ganglia, in which GDNF and GFRα-1 could be involved in cross-talk between neurons and SGCs. |
topic |
Pelvic ganglia Nerve growth factors Neuronal plasticity Reproduction Lower urogenital tract |
url |
http://www.einj.org/upload/pdf/inj-1834974-487.pdf |
work_keys_str_mv |
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