The role of alveolar type II cells in swine leptospirosis
Abstract: This study aimed to investigate a possible relationship between alveolar type II cells and the inflammatory response to infection with Leptospira spp., and thus comprise a further element that can be involved in the pathogenesis of lung injury in naturally infected pigs. The study group co...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Colégio Brasileiro de Patologia Animal (CBPA)
2015-07-01
|
Series: | Pesquisa Veterinária Brasileira |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000700620&lng=en&tlng=en |
id |
doaj-8b36c44b5e6f4209b6894d661d391078 |
---|---|
record_format |
Article |
spelling |
doaj-8b36c44b5e6f4209b6894d661d3910782020-11-25T00:05:04ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51502015-07-0135762062610.1590/S0100-736X2015000700004S0100-736X2015000700620The role of alveolar type II cells in swine leptospirosisÂngela P. CamposDayane F.H. MirandaGeórgia B.B. AlvesMicherlene S. CarneiroMaria G. PriantiLarissa M.F. GonçalvesVanessa CastroFrancisco A.L. CostaAbstract: This study aimed to investigate a possible relationship between alveolar type II cells and the inflammatory response to infection with Leptospira spp., and thus comprise a further element that can be involved in the pathogenesis of lung injury in naturally infected pigs. The study group consisted of 73 adult pigs that were extensively reared and slaughtered in Teresina, Piauí state, and Timon, Maranhão state, Brazil. The diagnosis of leptospirosis was made using the microscopic agglutination test (MAT) aided by immunohistochemistry and polymerase chain reaction. The MAT registered the occurrence of anti-Leptospira antibodies in 10.96% (8/73) of the pigs. Immunohistochemistry allowed for the visualization of the Leptospira spp. antigen in the lungs of 87.67% (64/73) of the pigs. There was hyperplasia of bronchus-associated lymphoid tissue and circulatory changes, such as congestion of alveolar septa, parenchymal hemorrhage and edema within the alveoli. Lung inflammation was more intense (p = 0.0312) in infected animals, which also showed increased thickening of the alveolar septa (p = 0.0006). Evaluation of alveolar type II (ATII) cells using an anti-TTF-1 (Thyroid Transcription Factor-1) antibody showed that there were more immunostained cells in the non-infected pigs (53.8%) than in the infected animals (46.2%) and that there was an inverse correlation between TTF-1 positive cells and the inflammatory infiltrate. There was no amplification of Leptospira DNA in the lung samples, but leptospiral DNA amplification was observed in the kidneys. The results of this study showed that a relationship exists between a decrease in alveolar type II cells and a leptospire infection. Thus, this work points to the importance of studying the ATII cells as a potential marker of the level of lung innate immune response during leptospirosis in pigs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000700620&lng=en&tlng=enCélulas alveolares tipo IIimunoistoquímicaLeptospira spp.pulmãosuíno. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ângela P. Campos Dayane F.H. Miranda Geórgia B.B. Alves Micherlene S. Carneiro Maria G. Prianti Larissa M.F. Gonçalves Vanessa Castro Francisco A.L. Costa |
spellingShingle |
Ângela P. Campos Dayane F.H. Miranda Geórgia B.B. Alves Micherlene S. Carneiro Maria G. Prianti Larissa M.F. Gonçalves Vanessa Castro Francisco A.L. Costa The role of alveolar type II cells in swine leptospirosis Pesquisa Veterinária Brasileira Células alveolares tipo II imunoistoquímica Leptospira spp. pulmão suíno. |
author_facet |
Ângela P. Campos Dayane F.H. Miranda Geórgia B.B. Alves Micherlene S. Carneiro Maria G. Prianti Larissa M.F. Gonçalves Vanessa Castro Francisco A.L. Costa |
author_sort |
Ângela P. Campos |
title |
The role of alveolar type II cells in swine leptospirosis |
title_short |
The role of alveolar type II cells in swine leptospirosis |
title_full |
The role of alveolar type II cells in swine leptospirosis |
title_fullStr |
The role of alveolar type II cells in swine leptospirosis |
title_full_unstemmed |
The role of alveolar type II cells in swine leptospirosis |
title_sort |
role of alveolar type ii cells in swine leptospirosis |
publisher |
Colégio Brasileiro de Patologia Animal (CBPA) |
series |
Pesquisa Veterinária Brasileira |
issn |
1678-5150 |
publishDate |
2015-07-01 |
description |
Abstract: This study aimed to investigate a possible relationship between alveolar type II cells and the inflammatory response to infection with Leptospira spp., and thus comprise a further element that can be involved in the pathogenesis of lung injury in naturally infected pigs. The study group consisted of 73 adult pigs that were extensively reared and slaughtered in Teresina, Piauí state, and Timon, Maranhão state, Brazil. The diagnosis of leptospirosis was made using the microscopic agglutination test (MAT) aided by immunohistochemistry and polymerase chain reaction. The MAT registered the occurrence of anti-Leptospira antibodies in 10.96% (8/73) of the pigs. Immunohistochemistry allowed for the visualization of the Leptospira spp. antigen in the lungs of 87.67% (64/73) of the pigs. There was hyperplasia of bronchus-associated lymphoid tissue and circulatory changes, such as congestion of alveolar septa, parenchymal hemorrhage and edema within the alveoli. Lung inflammation was more intense (p = 0.0312) in infected animals, which also showed increased thickening of the alveolar septa (p = 0.0006). Evaluation of alveolar type II (ATII) cells using an anti-TTF-1 (Thyroid Transcription Factor-1) antibody showed that there were more immunostained cells in the non-infected pigs (53.8%) than in the infected animals (46.2%) and that there was an inverse correlation between TTF-1 positive cells and the inflammatory infiltrate. There was no amplification of Leptospira DNA in the lung samples, but leptospiral DNA amplification was observed in the kidneys. The results of this study showed that a relationship exists between a decrease in alveolar type II cells and a leptospire infection. Thus, this work points to the importance of studying the ATII cells as a potential marker of the level of lung innate immune response during leptospirosis in pigs. |
topic |
Células alveolares tipo II imunoistoquímica Leptospira spp. pulmão suíno. |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000700620&lng=en&tlng=en |
work_keys_str_mv |
AT angelapcampos theroleofalveolartypeiicellsinswineleptospirosis AT dayanefhmiranda theroleofalveolartypeiicellsinswineleptospirosis AT georgiabbalves theroleofalveolartypeiicellsinswineleptospirosis AT micherlenescarneiro theroleofalveolartypeiicellsinswineleptospirosis AT mariagprianti theroleofalveolartypeiicellsinswineleptospirosis AT larissamfgoncalves theroleofalveolartypeiicellsinswineleptospirosis AT vanessacastro theroleofalveolartypeiicellsinswineleptospirosis AT franciscoalcosta theroleofalveolartypeiicellsinswineleptospirosis AT angelapcampos roleofalveolartypeiicellsinswineleptospirosis AT dayanefhmiranda roleofalveolartypeiicellsinswineleptospirosis AT georgiabbalves roleofalveolartypeiicellsinswineleptospirosis AT micherlenescarneiro roleofalveolartypeiicellsinswineleptospirosis AT mariagprianti roleofalveolartypeiicellsinswineleptospirosis AT larissamfgoncalves roleofalveolartypeiicellsinswineleptospirosis AT vanessacastro roleofalveolartypeiicellsinswineleptospirosis AT franciscoalcosta roleofalveolartypeiicellsinswineleptospirosis |
_version_ |
1725426403498786816 |