Vascular access infection by Staphylococcus aureus from removed dialysis accesses
Abstract Hemodialysis patients are particularly vulnerable to Staphylococcus aureus infection, with the vascular access serving as the site of entry for this formidable pathogen. Patients with arteriovenous grafts (AVGs) and tunneled‐cuffed catheters (TCCs) are at elevated risk of S. aureus infectio...
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doaj-8b54390b2b764b19ab215c482adfaef12020-11-25T00:49:59ZengWileyMicrobiologyOpen2045-88272019-08-0188n/an/a10.1002/mbo3.800Vascular access infection by Staphylococcus aureus from removed dialysis accessesChishih Chu0Min Yi Wong1Yuan‐Hsi Tseng2Chun‐Liang Lin3Chun‐Wu Tung4Chih‐Chen Kao5Yao‐Kuang Huang6Department of Microbiology, Immunology, and Biopharmaceuticals National Chiayi University Chiayi City Taiwan (R.O.C.)Division of Thoracic and Cardiovascular Surgery Chiayi Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Taoyuan Puzi City Chiayi County Taiwan (R.O.C.)Division of Thoracic and Cardiovascular Surgery Chiayi Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Taoyuan Puzi City Chiayi County Taiwan (R.O.C.)Department of Nephrology Chiayi Chang Gung Memorial Hospital Puzi City Chiayi County Taiwan (R.O.C.)Department of Nephrology Chiayi Chang Gung Memorial Hospital Puzi City Chiayi County Taiwan (R.O.C.)Division of Thoracic and Cardiovascular Surgery Chiayi Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Taoyuan Puzi City Chiayi County Taiwan (R.O.C.)Division of Thoracic and Cardiovascular Surgery Chiayi Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Taoyuan Puzi City Chiayi County Taiwan (R.O.C.)Abstract Hemodialysis patients are particularly vulnerable to Staphylococcus aureus infection, with the vascular access serving as the site of entry for this formidable pathogen. Patients with arteriovenous grafts (AVGs) and tunneled‐cuffed catheters (TCCs) are at elevated risk of S. aureus infection. In this study, we investigated the correlation between the clinical characteristics of S. aureus vascular access infection (VAI), molecular profiles, and the biofilm formation abilities of clinical isolates of S. aureus. We collected samples of methicillin‐resistant S. aureus (MRSA), methicillin‐sensitive S. aureus (MSSA), and methicillin‐sensitive S. argenteus (MSSAg) from patients with S. aureus VAI and patients with other infections. The molecular profiles of the clinical isolates were determined using disk diffusion testing and molecular typing. The biofilm formation ability was determined by microtiter plate assay. In total, 63 S. aureus and 10 S. argenteus isolates were identified: 40 MRSA, 23 MSSA, and ten MSSAg. MRSA was highly prevalent (77.8%) in TCC isolates and was multidrug resistant. Of the 40 MRSA isolates, ST239‐SCCmec III was the predominant clone. SCCmec type IV was the predominant type (35%) in isolates from AVGs, while SCCmec type III was prevalent in TCC infection and showed significantly higher biofilm formation ability than types IV and V. In dialysis VAI by S. aureus, patients with TCC were more often infected with MRSA than patients with AVG, and MRSA in TCC–VAI was predominantly SCCmec type III, which had the strongest drug resistance and biofilm formation ability.https://doi.org/10.1002/mbo3.800antibiotic resistancehemodialysisMRSAMSSASCCmecvascular access infection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chishih Chu Min Yi Wong Yuan‐Hsi Tseng Chun‐Liang Lin Chun‐Wu Tung Chih‐Chen Kao Yao‐Kuang Huang |
spellingShingle |
Chishih Chu Min Yi Wong Yuan‐Hsi Tseng Chun‐Liang Lin Chun‐Wu Tung Chih‐Chen Kao Yao‐Kuang Huang Vascular access infection by Staphylococcus aureus from removed dialysis accesses MicrobiologyOpen antibiotic resistance hemodialysis MRSA MSSA SCCmec vascular access infection |
author_facet |
Chishih Chu Min Yi Wong Yuan‐Hsi Tseng Chun‐Liang Lin Chun‐Wu Tung Chih‐Chen Kao Yao‐Kuang Huang |
author_sort |
Chishih Chu |
title |
Vascular access infection by Staphylococcus aureus from removed dialysis accesses |
title_short |
Vascular access infection by Staphylococcus aureus from removed dialysis accesses |
title_full |
Vascular access infection by Staphylococcus aureus from removed dialysis accesses |
title_fullStr |
Vascular access infection by Staphylococcus aureus from removed dialysis accesses |
title_full_unstemmed |
Vascular access infection by Staphylococcus aureus from removed dialysis accesses |
title_sort |
vascular access infection by staphylococcus aureus from removed dialysis accesses |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2019-08-01 |
description |
Abstract Hemodialysis patients are particularly vulnerable to Staphylococcus aureus infection, with the vascular access serving as the site of entry for this formidable pathogen. Patients with arteriovenous grafts (AVGs) and tunneled‐cuffed catheters (TCCs) are at elevated risk of S. aureus infection. In this study, we investigated the correlation between the clinical characteristics of S. aureus vascular access infection (VAI), molecular profiles, and the biofilm formation abilities of clinical isolates of S. aureus. We collected samples of methicillin‐resistant S. aureus (MRSA), methicillin‐sensitive S. aureus (MSSA), and methicillin‐sensitive S. argenteus (MSSAg) from patients with S. aureus VAI and patients with other infections. The molecular profiles of the clinical isolates were determined using disk diffusion testing and molecular typing. The biofilm formation ability was determined by microtiter plate assay. In total, 63 S. aureus and 10 S. argenteus isolates were identified: 40 MRSA, 23 MSSA, and ten MSSAg. MRSA was highly prevalent (77.8%) in TCC isolates and was multidrug resistant. Of the 40 MRSA isolates, ST239‐SCCmec III was the predominant clone. SCCmec type IV was the predominant type (35%) in isolates from AVGs, while SCCmec type III was prevalent in TCC infection and showed significantly higher biofilm formation ability than types IV and V. In dialysis VAI by S. aureus, patients with TCC were more often infected with MRSA than patients with AVG, and MRSA in TCC–VAI was predominantly SCCmec type III, which had the strongest drug resistance and biofilm formation ability. |
topic |
antibiotic resistance hemodialysis MRSA MSSA SCCmec vascular access infection |
url |
https://doi.org/10.1002/mbo3.800 |
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