A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry
Abstract Background Sulfanilamides, quinolones, nitroimidazoles, tetracyclines, cephalosporins, macrolides, and β-lactam are common tools in agriculture and can be found in animal-based foods such as goat milk and goat dried milk. To evaluate the risk of these species, reliable analytical methods ar...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2021-03-01
|
Series: | Journal of Analytical Science and Technology |
Online Access: | https://doi.org/10.1186/s40543-021-00268-4 |
id |
doaj-8b595d8182d94d87a5f0d71db9c5b213 |
---|---|
record_format |
Article |
spelling |
doaj-8b595d8182d94d87a5f0d71db9c5b2132021-03-28T11:03:21ZengSpringerOpenJournal of Analytical Science and Technology2093-33712021-03-0112111610.1186/s40543-021-00268-4A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometryLu Zhang0Liang Shi1Qiang He2Ying Li3Technology Centre of Xi’an Customs District P. R. ChinaSchool of Electronic and Information Engineering, Xi’an Technological UniversityTechnology Centre of Xi’an Customs District P. R. ChinaTechnology Centre of Xi’an Customs District P. R. ChinaAbstract Background Sulfanilamides, quinolones, nitroimidazoles, tetracyclines, cephalosporins, macrolides, and β-lactam are common tools in agriculture and can be found in animal-based foods such as goat milk and goat dried milk. To evaluate the risk of these species, reliable analytical methods are needed for accurate concentration determination, especially in goat milk and goat dried milk. Method We describe a method based on PRiME extraction coupled with UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry to accomplish this task. Result Under optimal conditions, the limit of quantification for all antibiotics was 0.5–100 μg/L in goat milk and goat dried milk samples. The recoveries were 60.6–110.0% for goat milk and 60.1–109.6% for goat dried milk with a coefficient of variation less than 15%. The detection limits were 0.5–1.0 μg/kg. The limits of quantification for the analytes were 5.0–10.0 μg/kg. Finally, the method was used to screen veterinary antibiotics in 50 local goat milk and goat dried milk samples; metronidazole and enrofloxacin were detected in goat milk. Conclusion This method offers good reliability and the capacity for simultaneous detection can be used to detect residual contents and evaluate health risks in goat milk and goat dried milk.https://doi.org/10.1186/s40543-021-00268-4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lu Zhang Liang Shi Qiang He Ying Li |
spellingShingle |
Lu Zhang Liang Shi Qiang He Ying Li A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry Journal of Analytical Science and Technology |
author_facet |
Lu Zhang Liang Shi Qiang He Ying Li |
author_sort |
Lu Zhang |
title |
A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry |
title_short |
A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry |
title_full |
A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry |
title_fullStr |
A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry |
title_full_unstemmed |
A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry |
title_sort |
rapid multiclass method for antibiotic residues in goat dairy products by uplc-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry |
publisher |
SpringerOpen |
series |
Journal of Analytical Science and Technology |
issn |
2093-3371 |
publishDate |
2021-03-01 |
description |
Abstract Background Sulfanilamides, quinolones, nitroimidazoles, tetracyclines, cephalosporins, macrolides, and β-lactam are common tools in agriculture and can be found in animal-based foods such as goat milk and goat dried milk. To evaluate the risk of these species, reliable analytical methods are needed for accurate concentration determination, especially in goat milk and goat dried milk. Method We describe a method based on PRiME extraction coupled with UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry to accomplish this task. Result Under optimal conditions, the limit of quantification for all antibiotics was 0.5–100 μg/L in goat milk and goat dried milk samples. The recoveries were 60.6–110.0% for goat milk and 60.1–109.6% for goat dried milk with a coefficient of variation less than 15%. The detection limits were 0.5–1.0 μg/kg. The limits of quantification for the analytes were 5.0–10.0 μg/kg. Finally, the method was used to screen veterinary antibiotics in 50 local goat milk and goat dried milk samples; metronidazole and enrofloxacin were detected in goat milk. Conclusion This method offers good reliability and the capacity for simultaneous detection can be used to detect residual contents and evaluate health risks in goat milk and goat dried milk. |
url |
https://doi.org/10.1186/s40543-021-00268-4 |
work_keys_str_mv |
AT luzhang arapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT liangshi arapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT qianghe arapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT yingli arapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT luzhang rapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT liangshi rapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT qianghe rapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry AT yingli rapidmulticlassmethodforantibioticresiduesingoatdairyproductsbyuplcquadrupoleelectrostaticfieldorbitraphighresolutionmassspectrometry |
_version_ |
1724200502092103680 |