Cellular modulation of polymeric device surfaces: promise of adult stem cells for neuroprosthetics

• Main Authors: Anja eRichter, Charli eKruse, Andreas eMoser, Ulrich G. Hofmann, Sandra eDanner
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2011-10-01
• Series: Frontiers in Neuroscience
• Subjects: Fibrin; Gliosis; stem cell; Neural Prosthesis; Foreign body response; Polyimide
• Online Access: http://journal.frontiersin.org/Journal/10.3389/fnins.2011.00114/full

Minimizing the foreign body response is seen as one critical research strategy for implants especially when designed for immune-privileged organs like the brain. The context of this work is to improve deep brain stimulating devices used in a consistently growing spectrum of psychomotoric and psychiatric diseases mainly in form of stiff electrodes. Based on the compliance match hypothesis of biocompatibility we present another step forward using flexible implant materials covered with brain-mimicking layers. Therefore we covered two types of flexible polyimide films with glandular stem cells derived from pancreatic acini. Using Real Time-PCR and fluorescent immunocytochemistry we analyzed markers representing various cell types of all three germ layers and stemness. The results demonstrate on mRNA and protein level the unchanged differentiation potential of the cells on the polyimides. We additionally developed a fibrinous hydrogel coating to protect them against shear forces upon eventual implantation. By repeating previous analysis and additional metabolism tests for all stages we corroborate the validity of this improvement. Consequently we assume that a stem cell cover may provide a native, fully and actively integrating brain-mimicking interface to the neuropil.