Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>)
High-throughput and large-scale measurements of chlorophyll <i>a</i> fluorescence (ChlF) are of great interest to investigate the photosynthetic performance of plants in the field. Here, we tested the capability to rapidly, precisely, and simultaneously estimate the number of pulse-ampli...
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doaj-8b679ca07bab4ac1b6c7379004821b9d2020-11-25T01:49:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-01216441644110.3390/ijms21176441Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>)Lorenzo Cotrozzi0Giacomo Lorenzini1Cristina Nali2Elisa Pellegrini3Vincenzo Saponaro4Yasutomo Hoshika5Leila Arab6Heinz Rennenberg7Elena Paoletti8Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, ItalyInstitute of Research on Terrestrial Ecosystems, National Research Council of Italy, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence, ItalyChair of Tree Physiology, Institute of Forest Sciences, Albert-Ludwigs-University Freiburg, Georges-Köhler-Allee 53/54, 79110 Freiburg, GermanyChair of Tree Physiology, Institute of Forest Sciences, Albert-Ludwigs-University Freiburg, Georges-Köhler-Allee 53/54, 79110 Freiburg, GermanyInstitute of Research on Terrestrial Ecosystems, National Research Council of Italy, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence, ItalyHigh-throughput and large-scale measurements of chlorophyll <i>a</i> fluorescence (ChlF) are of great interest to investigate the photosynthetic performance of plants in the field. Here, we tested the capability to rapidly, precisely, and simultaneously estimate the number of pulse-amplitude-modulation ChlF parameters commonly calculated from both dark- and light-adapted leaves (an operation which usually takes tens of minutes) from the reflectance of hyperspectral data collected on light-adapted leaves of date palm seedlings chronically exposed in a FACE facility to three ozone (O<sub>3</sub>) concentrations (ambient air, AA; target 1.5 × AA O<sub>3</sub>, named as moderate O<sub>3</sub>, MO; target 2 × AA O<sub>3</sub>, named as elevated O<sub>3</sub>, EO) for 75 consecutive days. Leaf spectral measurements were paired with reference measurements of ChlF, and predictive spectral models were constructed using partial least squares regression. Most of the ChlF parameters were well predicted by spectroscopic models (average model goodness-of-fit for validation, <i>R<sup>2</sup></i>: 0.53–0.82). Furthermore, comparing the full-range spectral profiles (i.e., 400–2400 nm), it was possible to distinguish with high accuracy (81% of success) plants exposed to the different O<sub>3</sub> concentrations, especially those exposed to EO from those exposed to MO and AA. This was possible even in the absence of visible foliar injury and using a moderately O<sub>3</sub>-susceptible species like the date palm. The latter view is confirmed by the few variations of the ChlF parameters, that occurred only under EO. The results of the current study could be applied in several scientific fields, such as precision agriculture and plant phenotyping.https://www.mdpi.com/1422-0067/21/17/6441Fluorescence quenchinghyperspectral phenotypingpartial least squares regressionphotosystem II activitypulse-amplitude-modulation fluorometrysaturation pulse method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lorenzo Cotrozzi Giacomo Lorenzini Cristina Nali Elisa Pellegrini Vincenzo Saponaro Yasutomo Hoshika Leila Arab Heinz Rennenberg Elena Paoletti |
spellingShingle |
Lorenzo Cotrozzi Giacomo Lorenzini Cristina Nali Elisa Pellegrini Vincenzo Saponaro Yasutomo Hoshika Leila Arab Heinz Rennenberg Elena Paoletti Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>) International Journal of Molecular Sciences Fluorescence quenching hyperspectral phenotyping partial least squares regression photosystem II activity pulse-amplitude-modulation fluorometry saturation pulse method |
author_facet |
Lorenzo Cotrozzi Giacomo Lorenzini Cristina Nali Elisa Pellegrini Vincenzo Saponaro Yasutomo Hoshika Leila Arab Heinz Rennenberg Elena Paoletti |
author_sort |
Lorenzo Cotrozzi |
title |
Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>) |
title_short |
Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>) |
title_full |
Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>) |
title_fullStr |
Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>) |
title_full_unstemmed |
Hyperspectral Reflectance of Light-Adapted Leaves Can Predict Both Dark- and Light-Adapted Chl Fluorescence Parameters, and the Effects of Chronic Ozone Exposure on Date Palm (<i>Phoenix dactylifera</i>) |
title_sort |
hyperspectral reflectance of light-adapted leaves can predict both dark- and light-adapted chl fluorescence parameters, and the effects of chronic ozone exposure on date palm (<i>phoenix dactylifera</i>) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-09-01 |
description |
High-throughput and large-scale measurements of chlorophyll <i>a</i> fluorescence (ChlF) are of great interest to investigate the photosynthetic performance of plants in the field. Here, we tested the capability to rapidly, precisely, and simultaneously estimate the number of pulse-amplitude-modulation ChlF parameters commonly calculated from both dark- and light-adapted leaves (an operation which usually takes tens of minutes) from the reflectance of hyperspectral data collected on light-adapted leaves of date palm seedlings chronically exposed in a FACE facility to three ozone (O<sub>3</sub>) concentrations (ambient air, AA; target 1.5 × AA O<sub>3</sub>, named as moderate O<sub>3</sub>, MO; target 2 × AA O<sub>3</sub>, named as elevated O<sub>3</sub>, EO) for 75 consecutive days. Leaf spectral measurements were paired with reference measurements of ChlF, and predictive spectral models were constructed using partial least squares regression. Most of the ChlF parameters were well predicted by spectroscopic models (average model goodness-of-fit for validation, <i>R<sup>2</sup></i>: 0.53–0.82). Furthermore, comparing the full-range spectral profiles (i.e., 400–2400 nm), it was possible to distinguish with high accuracy (81% of success) plants exposed to the different O<sub>3</sub> concentrations, especially those exposed to EO from those exposed to MO and AA. This was possible even in the absence of visible foliar injury and using a moderately O<sub>3</sub>-susceptible species like the date palm. The latter view is confirmed by the few variations of the ChlF parameters, that occurred only under EO. The results of the current study could be applied in several scientific fields, such as precision agriculture and plant phenotyping. |
topic |
Fluorescence quenching hyperspectral phenotyping partial least squares regression photosystem II activity pulse-amplitude-modulation fluorometry saturation pulse method |
url |
https://www.mdpi.com/1422-0067/21/17/6441 |
work_keys_str_mv |
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