Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus.
Streptococcus agalactiae is the primary colonizer of the anogenital mucosa of up to 30% of healthy women and can infect newborns during delivery and cause severe sepsis and meningitis. Persistent colonization usually involves the formation of biofilm and increasing evidences indicate that in pathoge...
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2010-01-01
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doaj-8b8dec01152c4b3592d8c0fef708c9cf2020-11-24T22:04:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0152e921610.1371/journal.pone.0009216Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus.Cira Daniela RinaudoRoberto RosiniCesira L GaleottiFrancesco BertiFrancesca NecchiValerio ReguzziClaudia GhezzoJohn Laird TelfordGuido GrandiDomenico MaioneStreptococcus agalactiae is the primary colonizer of the anogenital mucosa of up to 30% of healthy women and can infect newborns during delivery and cause severe sepsis and meningitis. Persistent colonization usually involves the formation of biofilm and increasing evidences indicate that in pathogenic streptococci biofilm formation is mediated by pili. Recently, we have characterized pili distribution and conservation in 289 GBS clinical isolates and we have shown that GBS has three pilus types, 1, 2a and 2b encoded by three corresponding pilus islands, and that each strain carries one or two islands. Here we have investigated the capacity of these strains to form biofilms. We have found that most of the biofilm-formers carry pilus 2a, and using insertion and deletion mutants we have confirmed that pilus type 2a, but not pilus types 1 and 2b, confers biofilm-forming phenotype. We also show that deletion of the major ancillary protein of type 2a did not impair biofilm formation while the inactivation of the other ancillary protein and of the backbone protein completely abolished this phenotype. Furthermore, antibodies raised against pilus components inhibited bacterial adherence to solid surfaces, offering new strategies to prevent GBS infection by targeting bacteria during their initial attachment to host epithelial cells.http://europepmc.org/articles/PMC2821406?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cira Daniela Rinaudo Roberto Rosini Cesira L Galeotti Francesco Berti Francesca Necchi Valerio Reguzzi Claudia Ghezzo John Laird Telford Guido Grandi Domenico Maione |
spellingShingle |
Cira Daniela Rinaudo Roberto Rosini Cesira L Galeotti Francesco Berti Francesca Necchi Valerio Reguzzi Claudia Ghezzo John Laird Telford Guido Grandi Domenico Maione Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. PLoS ONE |
author_facet |
Cira Daniela Rinaudo Roberto Rosini Cesira L Galeotti Francesco Berti Francesca Necchi Valerio Reguzzi Claudia Ghezzo John Laird Telford Guido Grandi Domenico Maione |
author_sort |
Cira Daniela Rinaudo |
title |
Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. |
title_short |
Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. |
title_full |
Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. |
title_fullStr |
Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. |
title_full_unstemmed |
Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. |
title_sort |
specific involvement of pilus type 2a in biofilm formation in group b streptococcus. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-01-01 |
description |
Streptococcus agalactiae is the primary colonizer of the anogenital mucosa of up to 30% of healthy women and can infect newborns during delivery and cause severe sepsis and meningitis. Persistent colonization usually involves the formation of biofilm and increasing evidences indicate that in pathogenic streptococci biofilm formation is mediated by pili. Recently, we have characterized pili distribution and conservation in 289 GBS clinical isolates and we have shown that GBS has three pilus types, 1, 2a and 2b encoded by three corresponding pilus islands, and that each strain carries one or two islands. Here we have investigated the capacity of these strains to form biofilms. We have found that most of the biofilm-formers carry pilus 2a, and using insertion and deletion mutants we have confirmed that pilus type 2a, but not pilus types 1 and 2b, confers biofilm-forming phenotype. We also show that deletion of the major ancillary protein of type 2a did not impair biofilm formation while the inactivation of the other ancillary protein and of the backbone protein completely abolished this phenotype. Furthermore, antibodies raised against pilus components inhibited bacterial adherence to solid surfaces, offering new strategies to prevent GBS infection by targeting bacteria during their initial attachment to host epithelial cells. |
url |
http://europepmc.org/articles/PMC2821406?pdf=render |
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