Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa
Pseudomonas aeruginosa (P. aeruginosa) is a highly pathogenic bacteria involved in numerous diseases among which, are urinary tract infections (UTIs). The pyocyanin secreted as a virulence factor by this bacterium has many beneficial applications but its high cost remains an obstacle for its widespr...
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doaj-8b96aa71423e4939b776e11d126bf0502020-12-27T04:29:15ZengElsevierSaudi Journal of Biological Sciences1319-562X2021-01-012819931000Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosaRehab Mohammed Elbargisy0Department of Pharmaceutics, College of Pharmacy, Jouf University, Sakaka, Al-Jouf, Saudi Arabia; Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptPseudomonas aeruginosa (P. aeruginosa) is a highly pathogenic bacteria involved in numerous diseases among which, are urinary tract infections (UTIs). The pyocyanin secreted as a virulence factor by this bacterium has many beneficial applications but its high cost remains an obstacle for its widespread use. In this study, a total of fifty urine isolates were identified as P. aeruginosa. All strains produced pyocyanin pigment with a range of 1.3–31 µg/ml. The highest producer clinical strain P21 and the standard strain PA14 were used in optimization of pyocyanin production. Among tested media, king’s A fluid medium resulted in the highest yield of pyocyanin pigment followed by nutrient broth. Growth at 37 °C was superior in pyocyanin production than growth at 30 °C. Both shaking and longer incubation periods (3–4 days) improved pyocyanin production. The pyocyanin yield was indifferent upon growth of P21 at both pH 7 and pH 8. In conclusion, the optimum conditions for pyocyanin production are to use King’s A fluid medium of pH 7 and incubate the inoculated medium at 37 °C with shaking at 200 rpm for a period of three to four days.http://www.sciencedirect.com/science/article/pii/S1319562X20305933P. aeruginosaPyocyaninKing’s A mediumUrinary tract infections (UTIs) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rehab Mohammed Elbargisy |
spellingShingle |
Rehab Mohammed Elbargisy Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa Saudi Journal of Biological Sciences P. aeruginosa Pyocyanin King’s A medium Urinary tract infections (UTIs) |
author_facet |
Rehab Mohammed Elbargisy |
author_sort |
Rehab Mohammed Elbargisy |
title |
Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa |
title_short |
Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa |
title_full |
Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa |
title_fullStr |
Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa |
title_full_unstemmed |
Optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of Pseudomonas aeruginosa |
title_sort |
optimization of nutritional and environmental conditions for pyocyanin production by urine isolates of pseudomonas aeruginosa |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2021-01-01 |
description |
Pseudomonas aeruginosa (P. aeruginosa) is a highly pathogenic bacteria involved in numerous diseases among which, are urinary tract infections (UTIs). The pyocyanin secreted as a virulence factor by this bacterium has many beneficial applications but its high cost remains an obstacle for its widespread use. In this study, a total of fifty urine isolates were identified as P. aeruginosa. All strains produced pyocyanin pigment with a range of 1.3–31 µg/ml. The highest producer clinical strain P21 and the standard strain PA14 were used in optimization of pyocyanin production. Among tested media, king’s A fluid medium resulted in the highest yield of pyocyanin pigment followed by nutrient broth. Growth at 37 °C was superior in pyocyanin production than growth at 30 °C. Both shaking and longer incubation periods (3–4 days) improved pyocyanin production. The pyocyanin yield was indifferent upon growth of P21 at both pH 7 and pH 8. In conclusion, the optimum conditions for pyocyanin production are to use King’s A fluid medium of pH 7 and incubate the inoculated medium at 37 °C with shaking at 200 rpm for a period of three to four days. |
topic |
P. aeruginosa Pyocyanin King’s A medium Urinary tract infections (UTIs) |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X20305933 |
work_keys_str_mv |
AT rehabmohammedelbargisy optimizationofnutritionalandenvironmentalconditionsforpyocyaninproductionbyurineisolatesofpseudomonasaeruginosa |
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