The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus
Summary As a pathogenic fungus, Aspergillus flavus can produce carcinogenic aflatoxins (AFs), which poses a great threat to crops and animals. Msb2, the signalling mucin protein, is a part of mitogen‐activated protein kinase (MAPK) pathway which contributes to a range of physiological processes. In...
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Online Access: | https://doi.org/10.1111/1751-7915.13701 |
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doaj-8b9fcdaf98f0414f93277e7aa4c01b4b2021-03-25T09:30:33ZengWileyMicrobial Biotechnology1751-79152021-03-0114262864210.1111/1751-7915.13701The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavusLing Qin0Ding Li1Jiaru Zhao2Guang Yang3Yinchun Wang4Kunlong Yang5Elisabeth Tumukunde6Shihua Wang7Jun Yuan8Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaKey Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of Biopesticide and Chemical Biology of Education Ministry School of Life Sciences Fujian Agriculture and Forestry University Fuzhou350002ChinaSummary As a pathogenic fungus, Aspergillus flavus can produce carcinogenic aflatoxins (AFs), which poses a great threat to crops and animals. Msb2, the signalling mucin protein, is a part of mitogen‐activated protein kinase (MAPK) pathway which contributes to a range of physiological processes. In this study, the roles of membrane mucin Msb2 were explored in A. flavus by the application of gene disruption. The deletion of msb2 gene (Δmsb2) caused defects in vegetative growth, sporulation and sclerotia formation when compared to WT and complement strain (Δmsb2C) in A. flavus. Using thin‐layer chromatography (TLC) and high‐performance liquid chromatography (HPLC) analysis, it was found that deletion of msb2 down‐regulated aflatoxin B1 (AFB1) synthesis and decreased the infection capacity of A. flavus. Consistently, Msb2 responds to cell wall stress and osmotic stress by positively regulating the phosphorylation of MAP kinase. Notably, Δmsb2 mutant exhibited cell wall defect, and it was more sensitive to inhibitor caspofungin when compared to WT and Δmsb2C. Taking together, these results revealed that Msb2 plays key roles in morphological development process, stresses adaptation, secondary metabolism and pathogenicity in fungus A. flavus.https://doi.org/10.1111/1751-7915.13701 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ling Qin Ding Li Jiaru Zhao Guang Yang Yinchun Wang Kunlong Yang Elisabeth Tumukunde Shihua Wang Jun Yuan |
spellingShingle |
Ling Qin Ding Li Jiaru Zhao Guang Yang Yinchun Wang Kunlong Yang Elisabeth Tumukunde Shihua Wang Jun Yuan The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus Microbial Biotechnology |
author_facet |
Ling Qin Ding Li Jiaru Zhao Guang Yang Yinchun Wang Kunlong Yang Elisabeth Tumukunde Shihua Wang Jun Yuan |
author_sort |
Ling Qin |
title |
The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus |
title_short |
The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus |
title_full |
The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus |
title_fullStr |
The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus |
title_full_unstemmed |
The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus |
title_sort |
membrane mucin msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus aspergillus flavus |
publisher |
Wiley |
series |
Microbial Biotechnology |
issn |
1751-7915 |
publishDate |
2021-03-01 |
description |
Summary As a pathogenic fungus, Aspergillus flavus can produce carcinogenic aflatoxins (AFs), which poses a great threat to crops and animals. Msb2, the signalling mucin protein, is a part of mitogen‐activated protein kinase (MAPK) pathway which contributes to a range of physiological processes. In this study, the roles of membrane mucin Msb2 were explored in A. flavus by the application of gene disruption. The deletion of msb2 gene (Δmsb2) caused defects in vegetative growth, sporulation and sclerotia formation when compared to WT and complement strain (Δmsb2C) in A. flavus. Using thin‐layer chromatography (TLC) and high‐performance liquid chromatography (HPLC) analysis, it was found that deletion of msb2 down‐regulated aflatoxin B1 (AFB1) synthesis and decreased the infection capacity of A. flavus. Consistently, Msb2 responds to cell wall stress and osmotic stress by positively regulating the phosphorylation of MAP kinase. Notably, Δmsb2 mutant exhibited cell wall defect, and it was more sensitive to inhibitor caspofungin when compared to WT and Δmsb2C. Taking together, these results revealed that Msb2 plays key roles in morphological development process, stresses adaptation, secondary metabolism and pathogenicity in fungus A. flavus. |
url |
https://doi.org/10.1111/1751-7915.13701 |
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