Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder
Borderline personality disorder (BPD) is a serious psychic disease with a high risk for suicide. DNA methylation is a hallmark for aberrant epigenetic regulation and could be involved in the etiology of BPD. Previously, it has been reported that increased DNA methylation of neuropsychiatric genes is...
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doaj-8bd12192c6ef45609d63e346a984056a2020-11-24T22:57:38ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-01-011716710.3390/ijms17010067ijms17010067Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality DisorderStefanie Teschler0Julia Gotthardt1Gerhard Dammann2Reinhard H. Dammann3Institute for Genetics, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyInstitute for Genetics, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyPsychiatric Hospital, Psychiatric Services of Thurgovia, CH-8596 Münsterlingen, Switzerland and Department of Psychiatry, Paracelsus Medical University, A-5020 Salzburg, AustriaInstitute for Genetics, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyBorderline personality disorder (BPD) is a serious psychic disease with a high risk for suicide. DNA methylation is a hallmark for aberrant epigenetic regulation and could be involved in the etiology of BPD. Previously, it has been reported that increased DNA methylation of neuropsychiatric genes is found in the blood of patients with BPD compared to healthy controls. Here, we analyzed DNA methylation patterns of the ribosomal RNA gene (rDNA promoter region and 5′-external transcribed spacer/5′ETS) and the promoter of the proline rich membrane anchor 1 gene (PRIMA1) in peripheral blood samples of 24 female patients (mean age (33 ± 11) years) diagnosed with DSM-IV BPD and in 11 female controls (mean age (32 ± 7) years). A significant aberrant methylation of rDNA and PRIMA1 was revealed for BPD patients using pyrosequencing. For the promoter of PRIMA1, the average methylation of six CpG sites was 1.6-fold higher in BPD patients compared to controls. In contrast, the methylation levels of the rDNA promoter region and the 5′ETS were significantly lower (0.9-fold) in patients with BPD compared to controls. Thus, for nine CpGs located in the rDNA promoter region and for four CpGs at the 5′ETS decreased methylation was found in peripheral blood of patients compared to controls. Our results suggest that aberrant methylation of rDNA and PRIMA1 is associated with the pathogenesis of BPD.http://www.mdpi.com/1422-0067/17/1/67borderline personality disorderribosomal RNA geneproline rich membrane anchor geneepigeneticsDNA methylation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefanie Teschler Julia Gotthardt Gerhard Dammann Reinhard H. Dammann |
spellingShingle |
Stefanie Teschler Julia Gotthardt Gerhard Dammann Reinhard H. Dammann Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder International Journal of Molecular Sciences borderline personality disorder ribosomal RNA gene proline rich membrane anchor gene epigenetics DNA methylation |
author_facet |
Stefanie Teschler Julia Gotthardt Gerhard Dammann Reinhard H. Dammann |
author_sort |
Stefanie Teschler |
title |
Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder |
title_short |
Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder |
title_full |
Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder |
title_fullStr |
Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder |
title_full_unstemmed |
Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder |
title_sort |
aberrant dna methylation of rdna and prima1 in borderline personality disorder |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2016-01-01 |
description |
Borderline personality disorder (BPD) is a serious psychic disease with a high risk for suicide. DNA methylation is a hallmark for aberrant epigenetic regulation and could be involved in the etiology of BPD. Previously, it has been reported that increased DNA methylation of neuropsychiatric genes is found in the blood of patients with BPD compared to healthy controls. Here, we analyzed DNA methylation patterns of the ribosomal RNA gene (rDNA promoter region and 5′-external transcribed spacer/5′ETS) and the promoter of the proline rich membrane anchor 1 gene (PRIMA1) in peripheral blood samples of 24 female patients (mean age (33 ± 11) years) diagnosed with DSM-IV BPD and in 11 female controls (mean age (32 ± 7) years). A significant aberrant methylation of rDNA and PRIMA1 was revealed for BPD patients using pyrosequencing. For the promoter of PRIMA1, the average methylation of six CpG sites was 1.6-fold higher in BPD patients compared to controls. In contrast, the methylation levels of the rDNA promoter region and the 5′ETS were significantly lower (0.9-fold) in patients with BPD compared to controls. Thus, for nine CpGs located in the rDNA promoter region and for four CpGs at the 5′ETS decreased methylation was found in peripheral blood of patients compared to controls. Our results suggest that aberrant methylation of rDNA and PRIMA1 is associated with the pathogenesis of BPD. |
topic |
borderline personality disorder ribosomal RNA gene proline rich membrane anchor gene epigenetics DNA methylation |
url |
http://www.mdpi.com/1422-0067/17/1/67 |
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