Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves
Diabetes Mellitus is an endocrine disorder which causes insulin deficiency. Medicinal plants are documented to be efficacious in the management of the disease. The current research set to determine the phytochemicals present, anti-oxidant activity and investigate the potency of Eriobotrya japonica a...
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doaj-8be2100f35e8400a886effb3282f425c2020-11-25T03:25:30ZengElsevierHeliyon2405-84402020-08-0168e04736Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leavesLebogang Mogole0Wesley Omwoyo1Fanyana Mtunzi2Department of Chemistry, Vaal University of Technology, Private Bag X021, Vandebijlpark, 1900, South AfricaDepartment of Chemistry, Vaal University of Technology, Private Bag X021, Vandebijlpark, 1900, South Africa; Department of Chemistry, Maasai Mara University, P.O Box 861-20500, Narok, Kenya; Corresponding author.Department of Chemistry, Vaal University of Technology, Private Bag X021, Vandebijlpark, 1900, South AfricaDiabetes Mellitus is an endocrine disorder which causes insulin deficiency. Medicinal plants are documented to be efficacious in the management of the disease. The current research set to determine the phytochemicals present, anti-oxidant activity and investigate the potency of Eriobotrya japonica against α-amylase inhibition. The leaves of the plant were extracted sequentially. The different extracts were evaluated for the presence of phytochemicals and their potential anti-oxidant and α-amylase inhibition activity. Methanol with the highest polarity, gave the highest yield of 20% and hexane with the lowest polarity have the lowest yield of 2.09%. This trend resembled that observed for the total flavanoid and total phenolic content analysis which gave values of 0.3822 mg QAE/mg and 3.810 mg GAE/mg respectively for methanolic and hexane extracts. The extracts of methanol recorded higher DPPH free radical scavenging activity of 87% and gave the lowest IC50 of 0.5336 which was below that of ascorbic acid used as a control. Hexane extract had a higher α-amylase inhibitory activity of 24% at 1 μg/ml as compared to other extracts. Generally hexane extracts of Eriobotrya japonica exhibits mild inhibitory activity against α-amylase enzyme which is recommended than the conventional therapy which maximally inhibits the enzyme causing major side effects. The results obtained herein support the use of the plant as an anti-diabetic agent at higher concentrations.http://www.sciencedirect.com/science/article/pii/S2405844020315796Food scienceFood analysisPharmaceutical chemistryDiabetesα-amylaseHyperglycaemia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lebogang Mogole Wesley Omwoyo Fanyana Mtunzi |
spellingShingle |
Lebogang Mogole Wesley Omwoyo Fanyana Mtunzi Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves Heliyon Food science Food analysis Pharmaceutical chemistry Diabetes α-amylase Hyperglycaemia |
author_facet |
Lebogang Mogole Wesley Omwoyo Fanyana Mtunzi |
author_sort |
Lebogang Mogole |
title |
Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves |
title_short |
Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves |
title_full |
Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves |
title_fullStr |
Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves |
title_full_unstemmed |
Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves |
title_sort |
phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (eriobotrya japonica) leaves |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2020-08-01 |
description |
Diabetes Mellitus is an endocrine disorder which causes insulin deficiency. Medicinal plants are documented to be efficacious in the management of the disease. The current research set to determine the phytochemicals present, anti-oxidant activity and investigate the potency of Eriobotrya japonica against α-amylase inhibition. The leaves of the plant were extracted sequentially. The different extracts were evaluated for the presence of phytochemicals and their potential anti-oxidant and α-amylase inhibition activity. Methanol with the highest polarity, gave the highest yield of 20% and hexane with the lowest polarity have the lowest yield of 2.09%. This trend resembled that observed for the total flavanoid and total phenolic content analysis which gave values of 0.3822 mg QAE/mg and 3.810 mg GAE/mg respectively for methanolic and hexane extracts. The extracts of methanol recorded higher DPPH free radical scavenging activity of 87% and gave the lowest IC50 of 0.5336 which was below that of ascorbic acid used as a control. Hexane extract had a higher α-amylase inhibitory activity of 24% at 1 μg/ml as compared to other extracts. Generally hexane extracts of Eriobotrya japonica exhibits mild inhibitory activity against α-amylase enzyme which is recommended than the conventional therapy which maximally inhibits the enzyme causing major side effects. The results obtained herein support the use of the plant as an anti-diabetic agent at higher concentrations. |
topic |
Food science Food analysis Pharmaceutical chemistry Diabetes α-amylase Hyperglycaemia |
url |
http://www.sciencedirect.com/science/article/pii/S2405844020315796 |
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