Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct
The bovine embryo develops in contact with the oviductal fluid (OF) during the first 4−5 days of pregnancy. The aim of this study was to decipher the protein interactions occurring between the developing embryo and surrounding OF. In-vitro produced 4−6 cell and morula embryos wer...
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doaj-8be9041a06d24345a8a4b087b542a31f2020-11-25T01:45:18ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121246610.3390/ijms21020466ijms21020466Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine OviductCharles Banliat0Guillaume Tsikis1Valérie Labas2Ana-Paula Teixeira-Gomes3Emmanuelle Com4Régis Lavigne5Charles Pineau6Benoit Guyonnet7Pascal Mermillod8Marie Saint-Dizier9INRAE, CNRS, Université de Tours, IFCE, UMR PRC, 37380 Nouzilly, FranceINRAE, CNRS, Université de Tours, IFCE, UMR PRC, 37380 Nouzilly, FranceINRAE, CNRS, Université de Tours, IFCE, UMR PRC, 37380 Nouzilly, FranceINRAE, Université de Tours, CHU de Tours, Plate-forme CIRE, PAIB, 37380 Nouzilly, FranceInserm, University of Rennes, EHESP, Irset (Institut de recherche en santé, environnement et travail)—UMR_S 1085, 35000 Rennes, FranceInserm, University of Rennes, EHESP, Irset (Institut de recherche en santé, environnement et travail)—UMR_S 1085, 35000 Rennes, FranceInserm, University of Rennes, EHESP, Irset (Institut de recherche en santé, environnement et travail)—UMR_S 1085, 35000 Rennes, FranceUnion Evolution, 35530 Noyal-sur-Vilaine, FranceINRAE, CNRS, Université de Tours, IFCE, UMR PRC, 37380 Nouzilly, FranceINRAE, CNRS, Université de Tours, IFCE, UMR PRC, 37380 Nouzilly, FranceThe bovine embryo develops in contact with the oviductal fluid (OF) during the first 4−5 days of pregnancy. The aim of this study was to decipher the protein interactions occurring between the developing embryo and surrounding OF. In-vitro produced 4−6 cell and morula embryos were incubated or not (controls) in post-ovulatory OF (OF-treated embryos) and proteins were then analyzed and quantified by high resolution mass spectrometry (MS) in both embryo groups and in OF. A comparative analysis of MS data allowed the identification and quantification of 56 embryo-interacting proteins originated from the OF, including oviductin (OVGP1) and several annexins (ANXA1, ANXA2, ANXA4) as the most abundant ones. Some embryo-interacting proteins were developmental stage-specific, showing a modulating role of the embryo in protein interactions. Three interacting proteins (OVGP1, ANXA1 and PYGL) were immunolocalized in the perivitelline space and in blastomeres, showing that OF proteins were able to cross the zona pellucida and be taken up by the embryo. Interacting proteins were involved in a wide range of functions, among which metabolism and cellular processes were predominant. This study identified for the first time a high number of oviductal embryo-interacting proteins, paving the way for further targeted studies of proteins potentially involved in the establishment of pregnancy in cattle.https://www.mdpi.com/1422-0067/21/2/466tubal fluidfallopian tubeoviductembryobovinecattleproteomicssecretionsmorula4–6 cell |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charles Banliat Guillaume Tsikis Valérie Labas Ana-Paula Teixeira-Gomes Emmanuelle Com Régis Lavigne Charles Pineau Benoit Guyonnet Pascal Mermillod Marie Saint-Dizier |
spellingShingle |
Charles Banliat Guillaume Tsikis Valérie Labas Ana-Paula Teixeira-Gomes Emmanuelle Com Régis Lavigne Charles Pineau Benoit Guyonnet Pascal Mermillod Marie Saint-Dizier Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct International Journal of Molecular Sciences tubal fluid fallopian tube oviduct embryo bovine cattle proteomics secretions morula 4–6 cell |
author_facet |
Charles Banliat Guillaume Tsikis Valérie Labas Ana-Paula Teixeira-Gomes Emmanuelle Com Régis Lavigne Charles Pineau Benoit Guyonnet Pascal Mermillod Marie Saint-Dizier |
author_sort |
Charles Banliat |
title |
Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct |
title_short |
Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct |
title_full |
Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct |
title_fullStr |
Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct |
title_full_unstemmed |
Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct |
title_sort |
identification of 56 proteins involved in embryo–maternal interactions in the bovine oviduct |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
The bovine embryo develops in contact with the oviductal fluid (OF) during the first 4−5 days of pregnancy. The aim of this study was to decipher the protein interactions occurring between the developing embryo and surrounding OF. In-vitro produced 4−6 cell and morula embryos were incubated or not (controls) in post-ovulatory OF (OF-treated embryos) and proteins were then analyzed and quantified by high resolution mass spectrometry (MS) in both embryo groups and in OF. A comparative analysis of MS data allowed the identification and quantification of 56 embryo-interacting proteins originated from the OF, including oviductin (OVGP1) and several annexins (ANXA1, ANXA2, ANXA4) as the most abundant ones. Some embryo-interacting proteins were developmental stage-specific, showing a modulating role of the embryo in protein interactions. Three interacting proteins (OVGP1, ANXA1 and PYGL) were immunolocalized in the perivitelline space and in blastomeres, showing that OF proteins were able to cross the zona pellucida and be taken up by the embryo. Interacting proteins were involved in a wide range of functions, among which metabolism and cellular processes were predominant. This study identified for the first time a high number of oviductal embryo-interacting proteins, paving the way for further targeted studies of proteins potentially involved in the establishment of pregnancy in cattle. |
topic |
tubal fluid fallopian tube oviduct embryo bovine cattle proteomics secretions morula 4–6 cell |
url |
https://www.mdpi.com/1422-0067/21/2/466 |
work_keys_str_mv |
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