Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee
The aim of this study was to assess the effects of three strains of Bacillus thuringiensis (Bt) on the longevity of workers of Africanized honey bee. Solutions at a concentration of 3 × 108 spores.mL-1 (dosage) were prepared for each strain of Bt (IPS 82, BR 81, and BR 147). Three bioassays were per...
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Universidade Estadual de Londrina
2018-02-01
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doaj-8bf8be0bcc0a4ee3a203982b76085acb2020-11-24T22:23:22ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592018-02-0139132933810.5433/1679-0359.2018v39n1p32916885Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey beeGabriela Libardoni0Alfredo de Gouvea1Fabiana Martins Costa-Maia2Everton Ricardi Lozano3Patrícia Franchi de Freitas4Fernanda Caroline Colombo5Fernanda Raulino6Rodrigo Mendes Antunes Maciel7Michele Potrich8Universidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáUniversidade Tecnológica Federal do ParanáThe aim of this study was to assess the effects of three strains of Bacillus thuringiensis (Bt) on the longevity of workers of Africanized honey bee. Solutions at a concentration of 3 × 108 spores.mL-1 (dosage) were prepared for each strain of Bt (IPS 82, BR 81, and BR 147). Three bioassays were performed as follows: spraying on the bees, contact with the sprayed surface, and candy paste incorporated with Bt. The bees of the Bt bioassay were submitted to histological analysis of the mesenteron. The longevity of workers was assessed from one to 120 hours using different ranges. It was found that the bees that were exposed to the strain of Bt IPS 82, in the spraying test, exhibited a reduced longevity. In the contact test, the BR 147 strain reduced the longevity of the bees. In the food test, in turn, the three studied strains reduced the longevity of the bees as follows: Bt IPS 82: 64.5 hours; Bt BR 81: 64.5 hours; and Bt BR 147: 60.0 hours. The Bt BR 81 strain was considered the most selective of the evaluated strains on Apis mellifera, reducing the longevity of this bee only when it came into contact by the method of ingestion.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/29090Africanized honey beeEntomopathogenic bacteriaSelectivity. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabriela Libardoni Alfredo de Gouvea Fabiana Martins Costa-Maia Everton Ricardi Lozano Patrícia Franchi de Freitas Fernanda Caroline Colombo Fernanda Raulino Rodrigo Mendes Antunes Maciel Michele Potrich |
spellingShingle |
Gabriela Libardoni Alfredo de Gouvea Fabiana Martins Costa-Maia Everton Ricardi Lozano Patrícia Franchi de Freitas Fernanda Caroline Colombo Fernanda Raulino Rodrigo Mendes Antunes Maciel Michele Potrich Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee Semina: Ciências Agrárias Africanized honey bee Entomopathogenic bacteria Selectivity. |
author_facet |
Gabriela Libardoni Alfredo de Gouvea Fabiana Martins Costa-Maia Everton Ricardi Lozano Patrícia Franchi de Freitas Fernanda Caroline Colombo Fernanda Raulino Rodrigo Mendes Antunes Maciel Michele Potrich |
author_sort |
Gabriela Libardoni |
title |
Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee |
title_short |
Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee |
title_full |
Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee |
title_fullStr |
Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee |
title_full_unstemmed |
Effect of different Bacillus thuringiensis strains on the longevity of Africanized honey bee |
title_sort |
effect of different bacillus thuringiensis strains on the longevity of africanized honey bee |
publisher |
Universidade Estadual de Londrina |
series |
Semina: Ciências Agrárias |
issn |
1676-546X 1679-0359 |
publishDate |
2018-02-01 |
description |
The aim of this study was to assess the effects of three strains of Bacillus thuringiensis (Bt) on the longevity of workers of Africanized honey bee. Solutions at a concentration of 3 × 108 spores.mL-1 (dosage) were prepared for each strain of Bt (IPS 82, BR 81, and BR 147). Three bioassays were performed as follows: spraying on the bees, contact with the sprayed surface, and candy paste incorporated with Bt. The bees of the Bt bioassay were submitted to histological analysis of the mesenteron. The longevity of workers was assessed from one to 120 hours using different ranges. It was found that the bees that were exposed to the strain of Bt IPS 82, in the spraying test, exhibited a reduced longevity. In the contact test, the BR 147 strain reduced the longevity of the bees. In the food test, in turn, the three studied strains reduced the longevity of the bees as follows: Bt IPS 82: 64.5 hours; Bt BR 81: 64.5 hours; and Bt BR 147: 60.0 hours. The Bt BR 81 strain was considered the most selective of the evaluated strains on Apis mellifera, reducing the longevity of this bee only when it came into contact by the method of ingestion. |
topic |
Africanized honey bee Entomopathogenic bacteria Selectivity. |
url |
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/29090 |
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