Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide

We showed previously that the <i>Schistosoma japonicum</i> insulin-like peptide (SjILP) binds the worm insulin receptors, thereby, activating the parasite’s insulin pathway and emphasizing its important role in regulating uptake of glucose, a nutrient essential for parasite sur...

Full description

Bibliographic Details
Main Authors:	Xiaofeng Du, Malcolm K. Jones, Sujeevi S. K. Nawaratna, Shiwanthi Ranasinghe, Chunrong Xiong, Pengfei Cai, Donald P. McManus, Hong You
Format:	Article
Language:	English
Published:	MDPI AG 2019-03-01
Series:	International Journal of Molecular Sciences
Subjects:	<i>Schistosoma japonicum</i> insulin-like peptide RNA inference immunolocalization diagnosis
Online Access:	https://www.mdpi.com/1422-0067/20/7/1565

id	doaj-8bfabce9dc7943c5837dfad343d5180c
record_format	Article
spelling	doaj-8bfabce9dc7943c5837dfad343d5180c2020-11-25T02:18:08ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-03-01207156510.3390/ijms20071565ijms20071565Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like PeptideXiaofeng Du0Malcolm K. Jones1Sujeevi S. K. Nawaratna2Shiwanthi Ranasinghe3Chunrong Xiong4Pengfei Cai5Donald P. McManus6Hong You7Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland 4006, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton 4343, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland 4006, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland 4006, AustraliaKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214000, ChinaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland 4006, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland 4006, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland 4006, AustraliaWe showed previously that the <i>Schistosoma japonicum</i> insulin-like peptide (SjILP) binds the worm insulin receptors, thereby, activating the parasite’s insulin pathway and emphasizing its important role in regulating uptake of glucose, a nutrient essential for parasite survival. Here we show that SjILP is differentially expressed in the schistosome life cycle and is especially highly transcribed in eggs, miracidia, and adult female worms. RNA inference was employed to knockdown SjILP in adults in vitro, with suppression confirmed by significantly reduced protein production, declined adenosine diphosphate levels, and reduction in glucose consumption. Immunolocalization showed that SjILP is located to lateral gland cells of mature intra-ovular miracidia in the schistosome egg, and is distributed on the ciliated epithelium and internal cell masses of newly transformed miracidia. In schistosomula, SjILP is present on the tegument in two antero-lateral points, indicating highly polarized expression during cercarial transformation. Analysis of serum from <i>S. japonicum</i>-infected mice by ELISA using a recombinant form of SjILP as an antigen revealed IgG immunoreactivity to this molecule at 7 weeks post-infection indicating it is likely secreted from mature eggs into the host circulation. These findings provide further insights on ILP function in schistosomes and its essential roles in parasite survival and growth in different development stages.https://www.mdpi.com/1422-0067/20/7/1565<i>Schistosoma japonicum</i>insulin-like peptideRNA inferenceimmunolocalizationdiagnosis
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xiaofeng Du Malcolm K. Jones Sujeevi S. K. Nawaratna Shiwanthi Ranasinghe Chunrong Xiong Pengfei Cai Donald P. McManus Hong You
spellingShingle	Xiaofeng Du Malcolm K. Jones Sujeevi S. K. Nawaratna Shiwanthi Ranasinghe Chunrong Xiong Pengfei Cai Donald P. McManus Hong You Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide International Journal of Molecular Sciences <i>Schistosoma japonicum</i> insulin-like peptide RNA inference immunolocalization diagnosis
author_facet	Xiaofeng Du Malcolm K. Jones Sujeevi S. K. Nawaratna Shiwanthi Ranasinghe Chunrong Xiong Pengfei Cai Donald P. McManus Hong You
author_sort	Xiaofeng Du
title	Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_short	Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_full	Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_fullStr	Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_full_unstemmed	Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_sort	gene expression in developmental stages of <i>schistosoma japonicum</i> provides further insight into the importance of the schistosome insulin-like peptide
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1422-0067
publishDate	2019-03-01
description	We showed previously that the <i>Schistosoma japonicum</i> insulin-like peptide (SjILP) binds the worm insulin receptors, thereby, activating the parasite’s insulin pathway and emphasizing its important role in regulating uptake of glucose, a nutrient essential for parasite survival. Here we show that SjILP is differentially expressed in the schistosome life cycle and is especially highly transcribed in eggs, miracidia, and adult female worms. RNA inference was employed to knockdown SjILP in adults in vitro, with suppression confirmed by significantly reduced protein production, declined adenosine diphosphate levels, and reduction in glucose consumption. Immunolocalization showed that SjILP is located to lateral gland cells of mature intra-ovular miracidia in the schistosome egg, and is distributed on the ciliated epithelium and internal cell masses of newly transformed miracidia. In schistosomula, SjILP is present on the tegument in two antero-lateral points, indicating highly polarized expression during cercarial transformation. Analysis of serum from <i>S. japonicum</i>-infected mice by ELISA using a recombinant form of SjILP as an antigen revealed IgG immunoreactivity to this molecule at 7 weeks post-infection indicating it is likely secreted from mature eggs into the host circulation. These findings provide further insights on ILP function in schistosomes and its essential roles in parasite survival and growth in different development stages.
topic	<i>Schistosoma japonicum</i> insulin-like peptide RNA inference immunolocalization diagnosis
url	https://www.mdpi.com/1422-0067/20/7/1565
work_keys_str_mv	AT xiaofengdu geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT malcolmkjones geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT sujeevisknawaratna geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT shiwanthiranasinghe geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT chunrongxiong geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT pengfeicai geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT donaldpmcmanus geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide AT hongyou geneexpressionindevelopmentalstagesofischistosomajaponicumiprovidesfurtherinsightintotheimportanceoftheschistosomeinsulinlikepeptide
_version_	1724883056517447680

Gene Expression in Developmental Stages of <i>Schistosoma japonicum</i> Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide

Similar Items