A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines
Psathyrostachys huashanica Keng is an endangered species that is endemic to China, which provides an important gene pool for wheat improvement. We developed a quick and reliable PCR-based diagnostic assay to accurately and efficiently detect P. huashanica DNA sequences from introgression lines, whic...
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2013-10-01
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doaj-8bfe456c5f4b44db86df78187fd401f72020-11-24T23:24:37ZengInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaSpanish Journal of Agricultural Research2171-92922013-10-011141094110010.5424/sjar/2013114-42451902A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative linesJ. Wang0M. Lu1W. L. Du2J. Zhang3X. Y. Dong4J. Wu5J. X. Zhao6Q. H. Yang7X. H. Chen8Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiShaanxi Key Laboratory of Genetic Engineering for Plant Breeding. College of Agronomy. Northwest A&F University. Yangling 712100, ShaanxiPsathyrostachys huashanica Keng is an endangered species that is endemic to China, which provides an important gene pool for wheat improvement. We developed a quick and reliable PCR-based diagnostic assay to accurately and efficiently detect P. huashanica DNA sequences from introgression lines, which was based on a species-specific marker derived from genomic DNA. The 900-bp PCR-amplified band used as a P. huashanica-specific RAPD marker was tested with 21 different plant species and was converted into a sequence-characterized amplified region (SCAR) marker by cloning and sequencing the selected fragments (pHs11). This SCAR marker, which was designated as RHS23, could clearly distinguish the presence of P. huashanica DNA repetitive sequences in wheat-P. huashanica derivative lines. The specificity of the marker was validated using 21 different plant species and a complete set of wheat-P. huashanica disomic addition lines (1Ns–7Ns, 2n=44=22II). This specific sequence targeted the Ns genome of P. huashanica and it was present in all the seven P. huashanica chromosomes. Therefore, this SCAR marker is specific for P. huashanica chromosomes and may be used in the identification of alien repetitive sequences in large gene pools. This diagnostic PCR assay for screening the target genetic material may play a key role in marker-assisted selective breeding programs.http://revistas.inia.es/index.php/sjar/article/view/4245addition linesmarker-assisted selectionrepetitive sequencesRAPDSCAR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J. Wang M. Lu W. L. Du J. Zhang X. Y. Dong J. Wu J. X. Zhao Q. H. Yang X. H. Chen |
spellingShingle |
J. Wang M. Lu W. L. Du J. Zhang X. Y. Dong J. Wu J. X. Zhao Q. H. Yang X. H. Chen A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines Spanish Journal of Agricultural Research addition lines marker-assisted selection repetitive sequences RAPD SCAR |
author_facet |
J. Wang M. Lu W. L. Du J. Zhang X. Y. Dong J. Wu J. X. Zhao Q. H. Yang X. H. Chen |
author_sort |
J. Wang |
title |
A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines |
title_short |
A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines |
title_full |
A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines |
title_fullStr |
A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines |
title_full_unstemmed |
A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines |
title_sort |
novel pcr-based marker for identifying ns chromosomes in wheat-psathyrostachys huashanica keng derivative lines |
publisher |
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria |
series |
Spanish Journal of Agricultural Research |
issn |
2171-9292 |
publishDate |
2013-10-01 |
description |
Psathyrostachys huashanica Keng is an endangered species that is endemic to China, which provides an important gene pool for wheat improvement. We developed a quick and reliable PCR-based diagnostic assay to accurately and efficiently detect P. huashanica DNA sequences from introgression lines, which was based on a species-specific marker derived from genomic DNA. The 900-bp PCR-amplified band used as a P. huashanica-specific RAPD marker was tested with 21 different plant species and was converted into a sequence-characterized amplified region (SCAR) marker by cloning and sequencing the selected fragments (pHs11). This SCAR marker, which was designated as RHS23, could clearly distinguish the presence of P. huashanica DNA repetitive sequences in wheat-P. huashanica derivative lines. The specificity of the marker was validated using 21 different plant species and a complete set of wheat-P. huashanica disomic addition lines (1Ns–7Ns, 2n=44=22II). This specific sequence targeted the Ns genome of P. huashanica and it was present in all the seven P. huashanica chromosomes. Therefore, this SCAR marker is specific for P. huashanica chromosomes and may be used in the identification of alien repetitive sequences in large gene pools. This diagnostic PCR assay for screening the target genetic material may play a key role in marker-assisted selective breeding programs. |
topic |
addition lines marker-assisted selection repetitive sequences RAPD SCAR |
url |
http://revistas.inia.es/index.php/sjar/article/view/4245 |
work_keys_str_mv |
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