M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results
Cardiovascular diseases represent a major issue in terms of morbidity and mortality for dialysis patients. This morbidity is due to the accelerated atherosclerosis observed in these patients. Atherosclerosis is a chronic inflammatory disease characterized by key players such as monocytes, macrophage...
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doaj-8c04d28ef5434921acccea86d18271742021-01-17T00:03:29ZengMDPI AGBiomedicines2227-90592021-01-019848410.3390/biomedicines9010084M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary ResultsValérie Pireaux0Cédric Delporte1Alexandre Rousseau2Jean-Marc Desmet3Pierre Van Antwerpen4Martine Raes5Karim Zouaoui Boudjeltia6URBC-Narilis, University of Namur, 61 rue de Bruxelles, 5000 Namur, BelgiumLaboratory of Pharmaceutical Chemistry and Analytical Platform, Faculty of Pharmacy, Université libre de Bruxelles (Campus de la Plaine) CP205/05, Boulevard du Triomphe, 1050 Brussels, BelgiumLaboratory of Experimental Medicine (ULB 222 Unit), CHU-Charleroi, ISPPC Hôpital Vésale, Université libre de Bruxelles, 6110 Montigny-Le-Tilleul, BelgiumNephrology-Hemodialysis Unit, CHU-Charleroi, ISPPC Hôpital Vésale, 6110 Montigny-Le-Tilleul, BelgiumLaboratory of Pharmaceutical Chemistry and Analytical Platform, Faculty of Pharmacy, Université libre de Bruxelles (Campus de la Plaine) CP205/05, Boulevard du Triomphe, 1050 Brussels, BelgiumURBC-Narilis, University of Namur, 61 rue de Bruxelles, 5000 Namur, BelgiumLaboratory of Experimental Medicine (ULB 222 Unit), CHU-Charleroi, ISPPC Hôpital Vésale, Université libre de Bruxelles, 6110 Montigny-Le-Tilleul, BelgiumCardiovascular diseases represent a major issue in terms of morbidity and mortality for dialysis patients. This morbidity is due to the accelerated atherosclerosis observed in these patients. Atherosclerosis is a chronic inflammatory disease characterized by key players such as monocytes, macrophages, or oxidized LDLs. Monocytes-macrophages are classified into subsets of polarized cells, with M1 and M2 macrophages considered, respectively, as pro- and anti-inflammatory. (1) Methods: The monocyte subsets and phenotypes were analyzed by flow cytometry. These data were completed by the quantification of plasma M-CSF, IL-8, CRP, Mox-LDLs, Apo-B, Apo-AI, chloro-tyrosine, and homocitrulline concentrations. The statistical differences and associations between two continuous variables were assessed using the Mann–Whitney U test and Spearman’s correlation coefficient, respectively. (2) Results: Hemodialyzed patients showed a significant increase in their concentrations of CRP, M-CSF, and IL-8 (inflammation biomarkers), as well as chloro-tyrosine and homocitrulline (myeloperoxidase-associated oxidative stress biomarkers). Moreover, we observed a higher percentage of M2 monocytes in the plasma of hemodialysis patients as compared to the controls. (3) Conclusions: Our data suggest that oxidative stress and an inflammatory environment, which is amplified in hemodialysis patients, seems to favor an increase in the concentration of circulating M-CSF, therefore leading to an increase in M2 polarization among circulating monocytes.https://www.mdpi.com/2227-9059/9/1/84dialysisinflammationmonocytesmyeloperoxidaseoxidationpolarization |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valérie Pireaux Cédric Delporte Alexandre Rousseau Jean-Marc Desmet Pierre Van Antwerpen Martine Raes Karim Zouaoui Boudjeltia |
spellingShingle |
Valérie Pireaux Cédric Delporte Alexandre Rousseau Jean-Marc Desmet Pierre Van Antwerpen Martine Raes Karim Zouaoui Boudjeltia M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results Biomedicines dialysis inflammation monocytes myeloperoxidase oxidation polarization |
author_facet |
Valérie Pireaux Cédric Delporte Alexandre Rousseau Jean-Marc Desmet Pierre Van Antwerpen Martine Raes Karim Zouaoui Boudjeltia |
author_sort |
Valérie Pireaux |
title |
M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results |
title_short |
M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results |
title_full |
M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results |
title_fullStr |
M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results |
title_full_unstemmed |
M2 Monocyte Polarization in Dialyzed Patients Is Associated with Increased Levels of M-CSF and Myeloperoxidase-Associated Oxidative Stress: Preliminary Results |
title_sort |
m2 monocyte polarization in dialyzed patients is associated with increased levels of m-csf and myeloperoxidase-associated oxidative stress: preliminary results |
publisher |
MDPI AG |
series |
Biomedicines |
issn |
2227-9059 |
publishDate |
2021-01-01 |
description |
Cardiovascular diseases represent a major issue in terms of morbidity and mortality for dialysis patients. This morbidity is due to the accelerated atherosclerosis observed in these patients. Atherosclerosis is a chronic inflammatory disease characterized by key players such as monocytes, macrophages, or oxidized LDLs. Monocytes-macrophages are classified into subsets of polarized cells, with M1 and M2 macrophages considered, respectively, as pro- and anti-inflammatory. (1) Methods: The monocyte subsets and phenotypes were analyzed by flow cytometry. These data were completed by the quantification of plasma M-CSF, IL-8, CRP, Mox-LDLs, Apo-B, Apo-AI, chloro-tyrosine, and homocitrulline concentrations. The statistical differences and associations between two continuous variables were assessed using the Mann–Whitney U test and Spearman’s correlation coefficient, respectively. (2) Results: Hemodialyzed patients showed a significant increase in their concentrations of CRP, M-CSF, and IL-8 (inflammation biomarkers), as well as chloro-tyrosine and homocitrulline (myeloperoxidase-associated oxidative stress biomarkers). Moreover, we observed a higher percentage of M2 monocytes in the plasma of hemodialysis patients as compared to the controls. (3) Conclusions: Our data suggest that oxidative stress and an inflammatory environment, which is amplified in hemodialysis patients, seems to favor an increase in the concentration of circulating M-CSF, therefore leading to an increase in M2 polarization among circulating monocytes. |
topic |
dialysis inflammation monocytes myeloperoxidase oxidation polarization |
url |
https://www.mdpi.com/2227-9059/9/1/84 |
work_keys_str_mv |
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