Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern
Background: Rheumatoid arthritis-associated interstitial lung disease (RA-ILD) occurs in 10%–30% of patients with RA, and interstitial lung disease (ILD) is associated with increased mortality in up to 10% of patients with RA. The pathogenesis of RA-ILD is virtually unknown. The aim of this study is...
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doaj-8c0dab1b7fc94dbd8ae4a828abcd90de2020-11-24T20:54:18ZengElsevierAllergology International1323-89302016-01-01651889510.1016/j.alit.2015.08.002Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia patternKozo SuharaYasunari MiyazakiTsukasa OkamotoMasahiro IshizukaKimitake TsuchiyaNaohiko InaseBackground: Rheumatoid arthritis-associated interstitial lung disease (RA-ILD) occurs in 10%–30% of patients with RA, and interstitial lung disease (ILD) is associated with increased mortality in up to 10% of patients with RA. The pathogenesis of RA-ILD is virtually unknown. The aim of this study is to investigate the proteins related to UIP pattern by comparing to OP pattern in RA-ILD using proteome analysis of bronchoalveolar lavage fluid (BALF). Methods: Proteomic differences in BALF were compared between the UIP pattern and OP pattern by examining BALF from 5 patients with the UIP pattern and 7 patients with the OP pattern by two-dimensional gel electrophoresis and mass spectrometry. Results: In individual comparisons of BALF samples, the levels of the protein gelsolin and Ig kappa chain C region were significantly higher in the UIP pattern than in the OP pattern. In contrast, the levels of α-1 antitrypsin, CRP, haptoglobin β, and surfactant protein A (isoform number 5) were all significantly higher in the OP pattern than in the UIP pattern. Gelsolin was cleaved into two fragments, a C-terminal half and N-terminal half, and the levels of both were significantly higher in the UIP pattern than in the OP pattern. Conclusions: Fragmented gelsolins may be associated with the pathogenesis of fibrosis in RA-ILD.http://www.sciencedirect.com/science/article/pii/S1323893015001756Bronchoalveolar lavage fluidGelsolinInterstitial pneumoniaProteomicsRheumatoid arthritis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kozo Suhara Yasunari Miyazaki Tsukasa Okamoto Masahiro Ishizuka Kimitake Tsuchiya Naohiko Inase |
spellingShingle |
Kozo Suhara Yasunari Miyazaki Tsukasa Okamoto Masahiro Ishizuka Kimitake Tsuchiya Naohiko Inase Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern Allergology International Bronchoalveolar lavage fluid Gelsolin Interstitial pneumonia Proteomics Rheumatoid arthritis |
author_facet |
Kozo Suhara Yasunari Miyazaki Tsukasa Okamoto Masahiro Ishizuka Kimitake Tsuchiya Naohiko Inase |
author_sort |
Kozo Suhara |
title |
Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern |
title_short |
Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern |
title_full |
Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern |
title_fullStr |
Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern |
title_full_unstemmed |
Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern |
title_sort |
fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2016-01-01 |
description |
Background: Rheumatoid arthritis-associated interstitial lung disease (RA-ILD) occurs in 10%–30% of patients with RA, and interstitial lung disease (ILD) is associated with increased mortality in up to 10% of patients with RA. The pathogenesis of RA-ILD is virtually unknown. The aim of this study is to investigate the proteins related to UIP pattern by comparing to OP pattern in RA-ILD using proteome analysis of bronchoalveolar lavage fluid (BALF).
Methods: Proteomic differences in BALF were compared between the UIP pattern and OP pattern by examining BALF from 5 patients with the UIP pattern and 7 patients with the OP pattern by two-dimensional gel electrophoresis and mass spectrometry.
Results: In individual comparisons of BALF samples, the levels of the protein gelsolin and Ig kappa chain C region were significantly higher in the UIP pattern than in the OP pattern. In contrast, the levels of α-1 antitrypsin, CRP, haptoglobin β, and surfactant protein A (isoform number 5) were all significantly higher in the OP pattern than in the UIP pattern. Gelsolin was cleaved into two fragments, a C-terminal half and N-terminal half, and the levels of both were significantly higher in the UIP pattern than in the OP pattern.
Conclusions: Fragmented gelsolins may be associated with the pathogenesis of fibrosis in RA-ILD. |
topic |
Bronchoalveolar lavage fluid Gelsolin Interstitial pneumonia Proteomics Rheumatoid arthritis |
url |
http://www.sciencedirect.com/science/article/pii/S1323893015001756 |
work_keys_str_mv |
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