Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides

<p>An experiment to study the influence of the chronic hormone LHRH-a and 17a-MT on haematology orange spot grouper, Epinephelus coioides broodstock was conducted at Gondol Research Institute for Mariculture, Bali.  Fish weights were ranged 4.7-10.4 kg (female) and 10.4-17.8 kg (male).  The fe...

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Main Authors: F. Johnny, A. Priyono, D. Roza
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2007-01-01
Series:Jurnal Akuakultur Indonesia
Online Access:http://journal.ipb.ac.id/index.php/jai/article/view/4008
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spelling doaj-8c1b39d4f43449249f35bb1f50cb8fee2020-11-24T22:32:10ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002007-01-0161273510.19027/jai.6.27-353617Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioidesF. Johnny0A. Priyono1D. Roza2Balai Besar Riset Perikanan Budidaya Laut Gondol PO BOX 140 – Singaraja 81101 – BaliBalai Besar Riset Perikanan Budidaya Laut Gondol PO BOX 140 – Singaraja 81101 – BaliBalai Besar Riset Perikanan Budidaya Laut Gondol PO BOX 140 – Singaraja 81101 – Bali<p>An experiment to study the influence of the chronic hormone LHRH-a and 17a-MT on haematology orange spot grouper, Epinephelus coioides broodstock was conducted at Gondol Research Institute for Mariculture, Bali.  Fish weights were ranged 4.7-10.4 kg (female) and 10.4-17.8 kg (male).  The females broodstock was treated by hormone 17a-MT with dosage 50 mg/kg body weight, and males was by hormone LHRH-a with dosage 50 and 100 mg/kg body weight.  Fish were kept on two concrete tank contained 100 m<sup>3</sup> sea water and 2 meters depth and density 15 fishes/tank,  male ratio and female was 1 : 2.  Hormone treatment was peformed every two months until six months culture.  Blood were collected before treatment by hormone and every two months.  Hemocytology parameters measured were hematocrit, hemoglobin, total erythrocyte, and total leucocyte.  Result of the average indicator of hemacytology to influence on haemological parameters.</p> <p>Keywords:  Epinephelus coioides, chronic hormone, hemocytology, orange spot grouper broodstock.</p> <p> </p> <h2>ABSTRAK</h2> <p>Suatu percobaan dengan tujuan untuk mengetahui pengaruh  hormon LHRH-a dan 17a-MT terhadap keragaan hemositologi induk ikan kerapu lumpur, <em>Epinephelus coioides</em> telah dilakukan di Balai Besar Riset Perikanan Budidaya Laut Gondol, Bali.  Ikan uji yang digunakan adalah induk ikan kerapu lumpur kelamin betina dengan bobot antara 4,7-10,4 kg dan induk kelamin jantan dengan bobot antara 10,4-17,8 kg.  Ikan uji dipelihara dalam bak beton volume 100 m<sup>3</sup> dengan kepadatan 15 ekor/bak, dilengkapi aerasi dan pergantian air sistim mengalir.  Induk kelamin betina diberikan perlakuan implantasi pelet hormon LHRH-a dosis 50 mg/kg bobot, dan induk kelamin jantan dengan hormon 17a-MT dosis 50 dan 100 mg/kg bobot ikan.  Implantasi dilakukan setiap 2 bulan, pada awal percobaan (sebelum perlakuan hormon/bulan ke-0), bulan ke-2, bulan ke-4, dan bulan ke-6 dilakukan sampling darah untuk pengujian keragaan hemositologi, meliputi; persentase hematokrit, kadar nilai hemoglobin, jumlah total eritrosit, dan jumlah total leukosit.   Hasil percobaan menunjukkan bahwa penggunaan hormon LHRH-a pada induk ikan kerapu lumpur kelamin betina dan hormon 17a-MT pada induk ikan kerapu lumpur kelamin jantan berpengaruh terhadap keragaan hemositologi.</p> <p>Kata kunci:  <em>Epinephelus coioides, </em> hemositologi, hormon kronik, induk ikan kerapu lumpur</p>http://journal.ipb.ac.id/index.php/jai/article/view/4008
collection DOAJ
language English
format Article
sources DOAJ
author F. Johnny
A. Priyono
D. Roza
spellingShingle F. Johnny
A. Priyono
D. Roza
Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
Jurnal Akuakultur Indonesia
author_facet F. Johnny
A. Priyono
D. Roza
author_sort F. Johnny
title Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
title_short Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
title_full Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
title_fullStr Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
title_full_unstemmed Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
title_sort study of the influence hormone lhrh-a and 17a-mt of hemocytology on orange spot grouper broodstock, epinephelus coioides
publisher Asosiasi Sains Akuakultur Indonesia
series Jurnal Akuakultur Indonesia
issn 1412-5269
2354-6700
publishDate 2007-01-01
description <p>An experiment to study the influence of the chronic hormone LHRH-a and 17a-MT on haematology orange spot grouper, Epinephelus coioides broodstock was conducted at Gondol Research Institute for Mariculture, Bali.  Fish weights were ranged 4.7-10.4 kg (female) and 10.4-17.8 kg (male).  The females broodstock was treated by hormone 17a-MT with dosage 50 mg/kg body weight, and males was by hormone LHRH-a with dosage 50 and 100 mg/kg body weight.  Fish were kept on two concrete tank contained 100 m<sup>3</sup> sea water and 2 meters depth and density 15 fishes/tank,  male ratio and female was 1 : 2.  Hormone treatment was peformed every two months until six months culture.  Blood were collected before treatment by hormone and every two months.  Hemocytology parameters measured were hematocrit, hemoglobin, total erythrocyte, and total leucocyte.  Result of the average indicator of hemacytology to influence on haemological parameters.</p> <p>Keywords:  Epinephelus coioides, chronic hormone, hemocytology, orange spot grouper broodstock.</p> <p> </p> <h2>ABSTRAK</h2> <p>Suatu percobaan dengan tujuan untuk mengetahui pengaruh  hormon LHRH-a dan 17a-MT terhadap keragaan hemositologi induk ikan kerapu lumpur, <em>Epinephelus coioides</em> telah dilakukan di Balai Besar Riset Perikanan Budidaya Laut Gondol, Bali.  Ikan uji yang digunakan adalah induk ikan kerapu lumpur kelamin betina dengan bobot antara 4,7-10,4 kg dan induk kelamin jantan dengan bobot antara 10,4-17,8 kg.  Ikan uji dipelihara dalam bak beton volume 100 m<sup>3</sup> dengan kepadatan 15 ekor/bak, dilengkapi aerasi dan pergantian air sistim mengalir.  Induk kelamin betina diberikan perlakuan implantasi pelet hormon LHRH-a dosis 50 mg/kg bobot, dan induk kelamin jantan dengan hormon 17a-MT dosis 50 dan 100 mg/kg bobot ikan.  Implantasi dilakukan setiap 2 bulan, pada awal percobaan (sebelum perlakuan hormon/bulan ke-0), bulan ke-2, bulan ke-4, dan bulan ke-6 dilakukan sampling darah untuk pengujian keragaan hemositologi, meliputi; persentase hematokrit, kadar nilai hemoglobin, jumlah total eritrosit, dan jumlah total leukosit.   Hasil percobaan menunjukkan bahwa penggunaan hormon LHRH-a pada induk ikan kerapu lumpur kelamin betina dan hormon 17a-MT pada induk ikan kerapu lumpur kelamin jantan berpengaruh terhadap keragaan hemositologi.</p> <p>Kata kunci:  <em>Epinephelus coioides, </em> hemositologi, hormon kronik, induk ikan kerapu lumpur</p>
url http://journal.ipb.ac.id/index.php/jai/article/view/4008
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