Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
Generating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2016-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11804 |
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doaj-8c2e27eaab1b4cc89a8d7d85fe9db2312021-05-11T11:14:07ZengNature Publishing GroupNature Communications2041-17232016-06-017111110.1038/ncomms11804Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichmentJustin A. Spanier0Daniel R. Frederick1Justin J. Taylor2James R. Heffernan3Dmitri I. Kotov4Tijana Martinov5Kevin C. Osum6Jenna L. Ruggiero7Blake J. Rust8Samuel J. Landry9Marc K. Jenkins10James B. McLachlan11Brian T. Fife12Department of Medicine, Center for Immunology, University of Minnesota Medical SchoolDepartment of Microbiology and Immunology, Tulane University School of MedicineDepartment of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical SchoolDepartment of Medicine, Center for Immunology, University of Minnesota Medical SchoolDepartment of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical SchoolDepartment of Medicine, Center for Immunology, University of Minnesota Medical SchoolDepartment of Medicine, Center for Immunology, University of Minnesota Medical SchoolDepartment of Medicine, Center for Immunology, University of Minnesota Medical SchoolDepartment of Biochemistry, Tulane University School of MedicineDepartment of Biochemistry, Tulane University School of MedicineDepartment of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical SchoolDepartment of Microbiology and Immunology, Tulane University School of MedicineDepartment of Medicine, Center for Immunology, University of Minnesota Medical SchoolGenerating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications.https://doi.org/10.1038/ncomms11804 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Justin A. Spanier Daniel R. Frederick Justin J. Taylor James R. Heffernan Dmitri I. Kotov Tijana Martinov Kevin C. Osum Jenna L. Ruggiero Blake J. Rust Samuel J. Landry Marc K. Jenkins James B. McLachlan Brian T. Fife |
| spellingShingle |
Justin A. Spanier Daniel R. Frederick Justin J. Taylor James R. Heffernan Dmitri I. Kotov Tijana Martinov Kevin C. Osum Jenna L. Ruggiero Blake J. Rust Samuel J. Landry Marc K. Jenkins James B. McLachlan Brian T. Fife Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment Nature Communications |
| author_facet |
Justin A. Spanier Daniel R. Frederick Justin J. Taylor James R. Heffernan Dmitri I. Kotov Tijana Martinov Kevin C. Osum Jenna L. Ruggiero Blake J. Rust Samuel J. Landry Marc K. Jenkins James B. McLachlan Brian T. Fife |
| author_sort |
Justin A. Spanier |
| title |
Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment |
| title_short |
Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment |
| title_full |
Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment |
| title_fullStr |
Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment |
| title_full_unstemmed |
Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment |
| title_sort |
efficient generation of monoclonal antibodies against peptide in the context of mhcii using magnetic enrichment |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2016-06-01 |
| description |
Generating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications. |
| url |
https://doi.org/10.1038/ncomms11804 |
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