A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine.
Enterotoxigenic Escherichia coli (ETEC) expressing F4 fimbria is the major pathogenic bacteria causing diarrhoea in neonatal and post-weaning piglets. Previous studies have revealed that the susceptibility to ETEC F4ab/F4ac is an autosomal Mendelian dominant trait and the loci controlling the F4ab/F...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3311625?pdf=render |
id |
doaj-8c33eaa3f31c4c82a2f8b3fca03ee9ff |
---|---|
record_format |
Article |
spelling |
doaj-8c33eaa3f31c4c82a2f8b3fca03ee9ff2020-11-25T02:51:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3212710.1371/journal.pone.0032127A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine.Wei-Xuan FuYang LiuXin LuXiao-Yan NiuXiang-Dong DingJian-Feng LiuQin ZhangEnterotoxigenic Escherichia coli (ETEC) expressing F4 fimbria is the major pathogenic bacteria causing diarrhoea in neonatal and post-weaning piglets. Previous studies have revealed that the susceptibility to ETEC F4ab/F4ac is an autosomal Mendelian dominant trait and the loci controlling the F4ab/F4ac receptor are located on SSC13q41, between markers SW207 and S0283. To pinpoint these loci and further validate previous findings, we performed a genome-wide association study (GWAS) using a two generation family-based population, consisting of 301 piglets with phenotypes of susceptibility to ETEC F4ab/F4ac by the vitro adhesion test. The DNA of all piglets and their parents was genotyped using the Illumina PorcineSNP60 BeadChip, and 50,972 and 50,483 SNPs were available for F4ab and F4ac susceptibility, respectively, in the association analysis after quality control. In summary, 28 and 18 significant SNPs (p<0.05) were detected associated with F4ab and F4ac susceptibility respectively at genome-wide significance level. From these significant findings, two novel candidate genes, HEG1 and ITGB5, were firstly identified as the most promising genes underlying F4ab/F4ac susceptibility in swine according to their functions and positions. Our findings herein provide a novel evidence for unravelling genetic mechanism of diarrhoea risk in piglets.http://europepmc.org/articles/PMC3311625?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei-Xuan Fu Yang Liu Xin Lu Xiao-Yan Niu Xiang-Dong Ding Jian-Feng Liu Qin Zhang |
spellingShingle |
Wei-Xuan Fu Yang Liu Xin Lu Xiao-Yan Niu Xiang-Dong Ding Jian-Feng Liu Qin Zhang A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine. PLoS ONE |
author_facet |
Wei-Xuan Fu Yang Liu Xin Lu Xiao-Yan Niu Xiang-Dong Ding Jian-Feng Liu Qin Zhang |
author_sort |
Wei-Xuan Fu |
title |
A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine. |
title_short |
A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine. |
title_full |
A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine. |
title_fullStr |
A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine. |
title_full_unstemmed |
A genome-wide association study identifies two novel promising candidate genes affecting Escherichia coli F4ab/F4ac susceptibility in swine. |
title_sort |
genome-wide association study identifies two novel promising candidate genes affecting escherichia coli f4ab/f4ac susceptibility in swine. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Enterotoxigenic Escherichia coli (ETEC) expressing F4 fimbria is the major pathogenic bacteria causing diarrhoea in neonatal and post-weaning piglets. Previous studies have revealed that the susceptibility to ETEC F4ab/F4ac is an autosomal Mendelian dominant trait and the loci controlling the F4ab/F4ac receptor are located on SSC13q41, between markers SW207 and S0283. To pinpoint these loci and further validate previous findings, we performed a genome-wide association study (GWAS) using a two generation family-based population, consisting of 301 piglets with phenotypes of susceptibility to ETEC F4ab/F4ac by the vitro adhesion test. The DNA of all piglets and their parents was genotyped using the Illumina PorcineSNP60 BeadChip, and 50,972 and 50,483 SNPs were available for F4ab and F4ac susceptibility, respectively, in the association analysis after quality control. In summary, 28 and 18 significant SNPs (p<0.05) were detected associated with F4ab and F4ac susceptibility respectively at genome-wide significance level. From these significant findings, two novel candidate genes, HEG1 and ITGB5, were firstly identified as the most promising genes underlying F4ab/F4ac susceptibility in swine according to their functions and positions. Our findings herein provide a novel evidence for unravelling genetic mechanism of diarrhoea risk in piglets. |
url |
http://europepmc.org/articles/PMC3311625?pdf=render |
work_keys_str_mv |
AT weixuanfu agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT yangliu agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT xinlu agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT xiaoyanniu agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT xiangdongding agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT jianfengliu agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT qinzhang agenomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT weixuanfu genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT yangliu genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT xinlu genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT xiaoyanniu genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT xiangdongding genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT jianfengliu genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine AT qinzhang genomewideassociationstudyidentifiestwonovelpromisingcandidategenesaffectingescherichiacolif4abf4acsusceptibilityinswine |
_version_ |
1724733629146333184 |