Cxcr4 expression is associated with time–course permanent and temporary myocardial infarction in rats

• Main Authors: Ali Asghar Kiani, Fereshteh Babaei, Mehrnoosh Sediqi, Azam Soleimani, Kolsum Ahmadi, Somayeh Shahrokhi, Khatereh Anbari, Afshin Nazari
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2017-06-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: CXCR4 gene expression; Time-course; Permanent cardiac ischemia; Temporary cardiac ischemia
• Online Access: http://ijbms.mums.ac.ir/article_8832_9766ee30b89428275c767b7be79826e8.pdf
• ISSN: 2008-3866; 2008-3874

Objective(s): Experimental myocardial infarction triggers secretion of Stromal cell-derived factor1 and lead to increase in the expression of its receptor "CXCR4" on the surface of various cells. The aim of this study was to evaluate the expression pattern of CXCR4 in peripheral blood cells following time-course permanent and temporary ischemia in rats. Materials and Methods: Fourteen male Wistar rats were divided into two groups of seven and were placed under permanent and transient ischemia. Peripheral blood mononuclear cells were isolated at different time points, RNAs extracted and applied to qRT-PCR analysis of the CXCR4 gene.  Results: Based on repeated measures analysis of variance, the differences in the expression levels of the gene in each of the groups were statistically significant over time (the effect of time) (P-value< 0.001). Additionally, the difference in gene expression between the two groups was statistically significant (the effect of group), such that at all times, the expression levels of the gene were significantly higher in the permanent ischemia than in the transient ischemia group (P-value