Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer
Xiaolin Pu,1,* Changqing Dong,2,* Wenyu Zhu1, Wei Li3, Hua Jiang1 1Department of Oncology, Changzhou Second People’s Hospital, Changzhou, Jiangsu Province, People’s Republic of China; 2Department of Thoracic Surgery, Nanjing Chest Hospital, Nanjing City, Jiangsu...
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doaj-8c63b9a0bde74559afe554881673a4652020-11-25T01:59:25ZengDove Medical PressOncoTargets and Therapy1178-69302019-09-01Volume 127361737348483Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancerPu XDong CZhu WLi WJiang HXiaolin Pu,1,* Changqing Dong,2,* Wenyu Zhu1, Wei Li3, Hua Jiang1 1Department of Oncology, Changzhou Second People’s Hospital, Changzhou, Jiangsu Province, People’s Republic of China; 2Department of Thoracic Surgery, Nanjing Chest Hospital, Nanjing City, Jiangsu Province, People’s Republic of China; 3Department of Oncology, Jiangsu Province People’s Hospital, Nanjing, Jiangsu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hua JiangDepartment of Oncology, Changzhou Second People’s Hospital, No. 29 Xinglong Lane, Tianning District, Changzhou, Jiangsu Province 213004, People’s Republic of ChinaTel +86 5 198 810 4931Email jianghua_hjia@163.comPurpose: Toll-like receptor 4 (TLR4) is involved in the inflammation in liver cancer. High-expressed stomatin-like protein 2 (SLP-2) is commonly reported in many cancer types. This study aims to investigate the functions of SLP-2 in TLR4-mediated inflammatory responses and tumor progression of liver cancer.Patients and methods: Plasmid transfection technique was applied to silence and overexpress genes. Changes in cell viability and apoptosis were determined by performing cell counting kit-8 assay and flow cytometry. The levels of pro-inflammatory cytokines were determined by ELISA. We further measured the several types of the malignant transformation of SK-Hep1 cells to assess the effects of SLP-2 silencing on the cell migration and invasion, proliferation and angiogenesis of liver cancer in vitro. Western blot and RT-qPCR were performed for expression analysis.Results: Lipopolysaccharide (LPS) promoted the cell proliferation of SK-Hep1 and production of tumor necrosis factor-α (TNF-α) and IL-6. SLP-2 silencing could inhibit the protein and mRNA levels of CD14 and Cdc42 and subsequently inhibited the levels of TNF-α and IL-6. Overexpressed CD14 not only remarkably reversed the proapoptotic ability of SLP-2 silencing and promoted the expression of Cdc42 and production of TNF-α and IL-6, but also notably reversed the inhibitory effects on the malignant abilities of SK-Hep1 cells by SLP-2 silencing.Conclusion: SLP-2 silencing could significantly attenuate the inflammatory responses and tumor progression of liver cancer via inhibiting LPS/TLR4 signal transduction through the repression of CD14.Keywords: liver cancer, stomatin-like protein 2, Toll-like receptor 4, apoptosis, inflammatory responsehttps://www.dovepress.com/silencing-stomatin-like-protein-2-attenuates-tumor-progression-and-inf-peer-reviewed-article-OTTLiver cancerStomatin-Like Protein 2Toll-like receptors 4ApoptosisInflammatory response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pu X Dong C Zhu W Li W Jiang H |
spellingShingle |
Pu X Dong C Zhu W Li W Jiang H Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer OncoTargets and Therapy Liver cancer Stomatin-Like Protein 2 Toll-like receptors 4 Apoptosis Inflammatory response |
author_facet |
Pu X Dong C Zhu W Li W Jiang H |
author_sort |
Pu X |
title |
Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer |
title_short |
Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer |
title_full |
Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer |
title_fullStr |
Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer |
title_full_unstemmed |
Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer |
title_sort |
silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing cd14 in liver cancer |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2019-09-01 |
description |
Xiaolin Pu,1,* Changqing Dong,2,* Wenyu Zhu1, Wei Li3, Hua Jiang1 1Department of Oncology, Changzhou Second People’s Hospital, Changzhou, Jiangsu Province, People’s Republic of China; 2Department of Thoracic Surgery, Nanjing Chest Hospital, Nanjing City, Jiangsu Province, People’s Republic of China; 3Department of Oncology, Jiangsu Province People’s Hospital, Nanjing, Jiangsu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hua JiangDepartment of Oncology, Changzhou Second People’s Hospital, No. 29 Xinglong Lane, Tianning District, Changzhou, Jiangsu Province 213004, People’s Republic of ChinaTel +86 5 198 810 4931Email jianghua_hjia@163.comPurpose: Toll-like receptor 4 (TLR4) is involved in the inflammation in liver cancer. High-expressed stomatin-like protein 2 (SLP-2) is commonly reported in many cancer types. This study aims to investigate the functions of SLP-2 in TLR4-mediated inflammatory responses and tumor progression of liver cancer.Patients and methods: Plasmid transfection technique was applied to silence and overexpress genes. Changes in cell viability and apoptosis were determined by performing cell counting kit-8 assay and flow cytometry. The levels of pro-inflammatory cytokines were determined by ELISA. We further measured the several types of the malignant transformation of SK-Hep1 cells to assess the effects of SLP-2 silencing on the cell migration and invasion, proliferation and angiogenesis of liver cancer in vitro. Western blot and RT-qPCR were performed for expression analysis.Results: Lipopolysaccharide (LPS) promoted the cell proliferation of SK-Hep1 and production of tumor necrosis factor-α (TNF-α) and IL-6. SLP-2 silencing could inhibit the protein and mRNA levels of CD14 and Cdc42 and subsequently inhibited the levels of TNF-α and IL-6. Overexpressed CD14 not only remarkably reversed the proapoptotic ability of SLP-2 silencing and promoted the expression of Cdc42 and production of TNF-α and IL-6, but also notably reversed the inhibitory effects on the malignant abilities of SK-Hep1 cells by SLP-2 silencing.Conclusion: SLP-2 silencing could significantly attenuate the inflammatory responses and tumor progression of liver cancer via inhibiting LPS/TLR4 signal transduction through the repression of CD14.Keywords: liver cancer, stomatin-like protein 2, Toll-like receptor 4, apoptosis, inflammatory response |
topic |
Liver cancer Stomatin-Like Protein 2 Toll-like receptors 4 Apoptosis Inflammatory response |
url |
https://www.dovepress.com/silencing-stomatin-like-protein-2-attenuates-tumor-progression-and-inf-peer-reviewed-article-OTT |
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