The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes
Abstract Background Cartilage destruction is the main characteristic of osteoarthritis (OA), and osteopontin (OPN) is elevated in OA articular cartilage; however, the reason for the increased OPN level is not determined. In addition, Wnt/β-catenin signaling participates in the progression of OA. The...
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doaj-8c76040ca00d45679fceb7eff2b6be022020-11-25T03:51:44ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2020-08-0115111110.1186/s13018-020-01881-6The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytesJian Tian0Shu-Guang Gao1Yu-Sheng Li2Chao Cheng3Zhen-Han Deng4Wei Luo5Fang-Jie Zhang6Department of Orthopaedics, Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, Yiyang Central Hospital, Clinical Medical Technology Demonstration Base for Minimally Invasive and Digital Orthopaedics in Hunan ProvinceDepartment of Sports Medicine, The First Hospital Affiliated to Shenzhen University, Shenzhen Second People’s HospitalDepartment of Orthopaedics, Xiangya Hospital, Central South UniversityDepartment of Emergency Medicine, Xiangya Hospital, Central South UniversityAbstract Background Cartilage destruction is the main characteristic of osteoarthritis (OA), and osteopontin (OPN) is elevated in OA articular cartilage; however, the reason for the increased OPN level is not determined. In addition, Wnt/β-catenin signaling participates in the progression of OA. The aim of the present study was to evaluate whether canonical Wnt signaling could regulate the expression of OPN in human chondrocytes in vitro. Methods Human chondrocytes were cultured in vitro, and we first assayed the mRNA levels of OPN and β-catenin in chondrocytes. Next, we performed transient transfection of TCF 4 shRNA into chondrocytes to inhibit TCF 4 expression and explore changes in the OPN level. Then, the Wnt/β-catenin signaling inhibitor Dickkopf-1 (Dkk-1) was incubated with chondrocytes, and we assayed the changes in β-catenin and OPN. Results Our results showed that the expression of both β-catenin and OPN was increased in OA chondrocytes, but there were no correlations between β-catenin and OPN expression. TCF4 shRNA downregulated the expression of TCF 4 and OPN in chondrocytes, while after treatment with rDKK-1 at a concentration of 400 ng/ml for 24 h, the mRNA and protein expression of both β-catenin and OPN was significantly decreased in chondrocytes. Conclusions Elevated OPN expression might be regulated by the β-catenin/TCF-4 pathway, and the Wnt/β-catenin inhibitor DKK1 could inhibit the expression of β-catenin and OPN in OA chondrocytes.http://link.springer.com/article/10.1186/s13018-020-01881-6β-CateninChondrocyteDickkopf 1OsteopontinTCF 4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jian Tian Shu-Guang Gao Yu-Sheng Li Chao Cheng Zhen-Han Deng Wei Luo Fang-Jie Zhang |
spellingShingle |
Jian Tian Shu-Guang Gao Yu-Sheng Li Chao Cheng Zhen-Han Deng Wei Luo Fang-Jie Zhang The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes Journal of Orthopaedic Surgery and Research β-Catenin Chondrocyte Dickkopf 1 Osteopontin TCF 4 |
author_facet |
Jian Tian Shu-Guang Gao Yu-Sheng Li Chao Cheng Zhen-Han Deng Wei Luo Fang-Jie Zhang |
author_sort |
Jian Tian |
title |
The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes |
title_short |
The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes |
title_full |
The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes |
title_fullStr |
The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes |
title_full_unstemmed |
The β-catenin/TCF-4 pathway regulates the expression of OPN in human osteoarthritic chondrocytes |
title_sort |
β-catenin/tcf-4 pathway regulates the expression of opn in human osteoarthritic chondrocytes |
publisher |
BMC |
series |
Journal of Orthopaedic Surgery and Research |
issn |
1749-799X |
publishDate |
2020-08-01 |
description |
Abstract Background Cartilage destruction is the main characteristic of osteoarthritis (OA), and osteopontin (OPN) is elevated in OA articular cartilage; however, the reason for the increased OPN level is not determined. In addition, Wnt/β-catenin signaling participates in the progression of OA. The aim of the present study was to evaluate whether canonical Wnt signaling could regulate the expression of OPN in human chondrocytes in vitro. Methods Human chondrocytes were cultured in vitro, and we first assayed the mRNA levels of OPN and β-catenin in chondrocytes. Next, we performed transient transfection of TCF 4 shRNA into chondrocytes to inhibit TCF 4 expression and explore changes in the OPN level. Then, the Wnt/β-catenin signaling inhibitor Dickkopf-1 (Dkk-1) was incubated with chondrocytes, and we assayed the changes in β-catenin and OPN. Results Our results showed that the expression of both β-catenin and OPN was increased in OA chondrocytes, but there were no correlations between β-catenin and OPN expression. TCF4 shRNA downregulated the expression of TCF 4 and OPN in chondrocytes, while after treatment with rDKK-1 at a concentration of 400 ng/ml for 24 h, the mRNA and protein expression of both β-catenin and OPN was significantly decreased in chondrocytes. Conclusions Elevated OPN expression might be regulated by the β-catenin/TCF-4 pathway, and the Wnt/β-catenin inhibitor DKK1 could inhibit the expression of β-catenin and OPN in OA chondrocytes. |
topic |
β-Catenin Chondrocyte Dickkopf 1 Osteopontin TCF 4 |
url |
http://link.springer.com/article/10.1186/s13018-020-01881-6 |
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