Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice
Acute pancreatitis is an inflammatory process of the pancreatic tissue that often leads to distant organ dysfunction. Although liver injury is uncommon in acute pancreatitis, obesity is a risk factor for the development of hepatic complications. The aim of this work was to evaluate the role of PGC-1...
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doaj-8c860817546c4dbe863d055e26bffb572020-11-25T03:02:41ZengMDPI AGAntioxidants2076-39212020-09-01988788710.3390/antiox9090887Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese MiceSergio Rius-Pérez0Isabel Torres-Cuevas1María Monsalve2Francisco J. Miranda3Salvador Pérez4Department of Physiology, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andres Estelles s/n, 46100 Burjassot, SpainNeonatal Research Group, Health Research Institute La Fe, 46026 Valencia, SpainInstituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Arturo Duperier, 4, 28029 Madrid, SpainDepartment of Physiology, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andres Estelles s/n, 46100 Burjassot, SpainDepartment of Physiology, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andres Estelles s/n, 46100 Burjassot, SpainAcute pancreatitis is an inflammatory process of the pancreatic tissue that often leads to distant organ dysfunction. Although liver injury is uncommon in acute pancreatitis, obesity is a risk factor for the development of hepatic complications. The aim of this work was to evaluate the role of PGC-1α in inflammatory response regulation in the liver and its contribution to the detrimental effect of obesity on the liver during acute pancreatitis. For this purpose, we induced acute pancreatitis by cerulein in not only wild-type (WT) and PGC-1α knockout (KO) mice, but also in lean and obese mice. PGC-1α levels were up-regulated in the mice livers with pancreatitis. The increased PGC-1α levels were bound to p65 to restrain its transcriptional activity toward <i>Nos2</i>. Lack of PGC-1α favored the assembly of the p65/phospho-STAT3 complex, which promoted <i>Nos2</i> expression during acute pancreatitis. The increased transcript <i>Nos2</i> levels and the pro-oxidant liver status caused by the down-regulated expression of the PGC-1α-dependent antioxidant genes enhanced nitrosative stress and decreased energy charge in the livers of the PGC-1α KO mice with pancreatitis. It is noteworthy that the PGC-1α levels lowered in the obese mice livers, which increased the <i>Nos2</i> mRNA expression and protein nitration levels and decreased energy charge during pancreatitis. In conclusion, obesity impairs PGC-1α up-regulation in the liver to cause nitrosative stress during acute pancreatitis.https://www.mdpi.com/2076-3921/9/9/887acute pancreatitisobesitynitrosative stressPGC-1αliver |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sergio Rius-Pérez Isabel Torres-Cuevas María Monsalve Francisco J. Miranda Salvador Pérez |
spellingShingle |
Sergio Rius-Pérez Isabel Torres-Cuevas María Monsalve Francisco J. Miranda Salvador Pérez Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice Antioxidants acute pancreatitis obesity nitrosative stress PGC-1α liver |
author_facet |
Sergio Rius-Pérez Isabel Torres-Cuevas María Monsalve Francisco J. Miranda Salvador Pérez |
author_sort |
Sergio Rius-Pérez |
title |
Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice |
title_short |
Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice |
title_full |
Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice |
title_fullStr |
Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice |
title_full_unstemmed |
Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice |
title_sort |
impairment of pgc-1 alpha up-regulation enhances nitrosative stress in the liver during acute pancreatitis in obese mice |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2020-09-01 |
description |
Acute pancreatitis is an inflammatory process of the pancreatic tissue that often leads to distant organ dysfunction. Although liver injury is uncommon in acute pancreatitis, obesity is a risk factor for the development of hepatic complications. The aim of this work was to evaluate the role of PGC-1α in inflammatory response regulation in the liver and its contribution to the detrimental effect of obesity on the liver during acute pancreatitis. For this purpose, we induced acute pancreatitis by cerulein in not only wild-type (WT) and PGC-1α knockout (KO) mice, but also in lean and obese mice. PGC-1α levels were up-regulated in the mice livers with pancreatitis. The increased PGC-1α levels were bound to p65 to restrain its transcriptional activity toward <i>Nos2</i>. Lack of PGC-1α favored the assembly of the p65/phospho-STAT3 complex, which promoted <i>Nos2</i> expression during acute pancreatitis. The increased transcript <i>Nos2</i> levels and the pro-oxidant liver status caused by the down-regulated expression of the PGC-1α-dependent antioxidant genes enhanced nitrosative stress and decreased energy charge in the livers of the PGC-1α KO mice with pancreatitis. It is noteworthy that the PGC-1α levels lowered in the obese mice livers, which increased the <i>Nos2</i> mRNA expression and protein nitration levels and decreased energy charge during pancreatitis. In conclusion, obesity impairs PGC-1α up-regulation in the liver to cause nitrosative stress during acute pancreatitis. |
topic |
acute pancreatitis obesity nitrosative stress PGC-1α liver |
url |
https://www.mdpi.com/2076-3921/9/9/887 |
work_keys_str_mv |
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