The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran
Background: Helicobacter pylori antimicrobial resistance is an important factor responsible for treatment failure. The purpose of this study was evaluating the prevalence of point mutations in clarithromycin-resistant clinical isolates of H. pylori in Isfahan city of Iran. Materials and Methods: Th...
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doaj-8c915616ee104919ace32c00203c30522020-11-24T23:09:13ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752014-01-0131989810.4103/2277-9175.129368The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - IranFarzad KhademiJamshid FaghriSharareh MoghimBahram Nasr EsfahaniHossein FazeliFarkhondeh PoursinaPeyman AdibiMasoumeh MadhiHajieh Ghasemian SafaeiBackground: Helicobacter pylori antimicrobial resistance is an important factor responsible for treatment failure. The purpose of this study was evaluating the prevalence of point mutations in clarithromycin-resistant clinical isolates of H. pylori in Isfahan city of Iran. Materials and Methods: Thirty isolates of H. pylori from 130 biopsy specimens were isolated by culture and confirmed by biochemical and PCR tests. The MIC of clarithromycin antibiotic for 30 clinical isolates of H. pylori was determined by E-test method. The point mutations in the 288 bp of 23S rRNA gene of H. Pylori were investigated in four clarithromycin-resistant clinical isolates by PCR followed by sequencing. Results: Among 30 isolates of H. pylori, 4 cases were resistant to clarithromycin. One point mutation was found at position T2243C in the 23S rRNA gene in all resistance isolates. Conclusions: In our study, H. pylori resistance to clarithromycin associated with point mutation at position 2243 (T2243C).http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=98;epage=98;aulast=KhademiClarithromycin resistanceHelicobacter pyloripoint mutations |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Farzad Khademi Jamshid Faghri Sharareh Moghim Bahram Nasr Esfahani Hossein Fazeli Farkhondeh Poursina Peyman Adibi Masoumeh Madhi Hajieh Ghasemian Safaei |
spellingShingle |
Farzad Khademi Jamshid Faghri Sharareh Moghim Bahram Nasr Esfahani Hossein Fazeli Farkhondeh Poursina Peyman Adibi Masoumeh Madhi Hajieh Ghasemian Safaei The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran Advanced Biomedical Research Clarithromycin resistance Helicobacter pylori point mutations |
author_facet |
Farzad Khademi Jamshid Faghri Sharareh Moghim Bahram Nasr Esfahani Hossein Fazeli Farkhondeh Poursina Peyman Adibi Masoumeh Madhi Hajieh Ghasemian Safaei |
author_sort |
Farzad Khademi |
title |
The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran |
title_short |
The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran |
title_full |
The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran |
title_fullStr |
The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran |
title_full_unstemmed |
The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran |
title_sort |
study of mutation in 23s rrna resistance gene of helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in isfahan - iran |
publisher |
Wolters Kluwer Medknow Publications |
series |
Advanced Biomedical Research |
issn |
2277-9175 2277-9175 |
publishDate |
2014-01-01 |
description |
Background: Helicobacter pylori antimicrobial resistance is an important factor responsible for treatment failure. The purpose of this study was evaluating the prevalence of point mutations in clarithromycin-resistant clinical isolates of H. pylori in Isfahan city of Iran.
Materials and Methods: Thirty isolates of H. pylori from 130 biopsy specimens were isolated by culture and confirmed by biochemical and PCR tests. The MIC of clarithromycin antibiotic for 30 clinical isolates of H. pylori was determined by E-test method. The point mutations in the 288 bp of 23S rRNA gene of H. Pylori were investigated in four clarithromycin-resistant clinical isolates by PCR followed by sequencing.
Results: Among 30 isolates of H. pylori, 4 cases were resistant to clarithromycin. One point mutation was found at position T2243C in the 23S rRNA gene in all resistance isolates.
Conclusions: In our study, H. pylori resistance to clarithromycin associated with point mutation at position 2243 (T2243C). |
topic |
Clarithromycin resistance Helicobacter pylori point mutations |
url |
http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=98;epage=98;aulast=Khademi |
work_keys_str_mv |
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