Stereospecificity of hydride transfer and molecular docking in FMN‐dependent NADH‐indigo reductase of Bacillus smithii

Stereospecificity of hydride transfer and molecular docking in FMN‐dependent NADH‐indigo reductase of Bacillus smithii

In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii. We performed 1H‐NMR spectroscopy using deuterium‐labeled NADH (4R‐2H‐NADH) a...

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Bibliographic Details
Main Authors: Kazunari Yoneda, Haruhiko Sakuraba, Tomohiro Araki, Toshihisa Ohshima
Format: Article
Language:English
Published: Wiley 2021-07-01
Series:FEBS Open Bio
Subjects:
H‐NMR
cofactor stereospecificity
FMN‐dependent NADH‐indigo reductase
molecular docking simulation
Online Access:https://doi.org/10.1002/2211-5463.13200

Internet

https://doi.org/10.1002/2211-5463.13200

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