Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage
In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 10<sup&...
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doaj-8cb2a18e35314f168bd1e487476521182021-04-02T13:41:30ZengMDPI AGAgriculture2077-04722019-12-011011010.3390/agriculture10010010agriculture10010010Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa SilageZhe Wu0Shengyang Xu1Ying Yun2Tingting Jia3Zhu Yu4College of Grassland Science and Technology, China Agricultural University, Beijing 100093, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing 100093, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing 100093, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing 100093, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing 100093, ChinaIn this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 10<sup>6</sup> colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as <i>Lactobacillus plantarum</i>. The characteristics of chemical and fermentation compounds indicated that PLA and the two strains efficiently improved the quality of the alfalfa silage. It can be concluded that the use of the strains and PLA can significantly improve the quality of silage.https://www.mdpi.com/2077-0472/10/1/103-phenyllactic acidalfalfa silage<i>lactobacillus plantarum</i>strain screening |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhe Wu Shengyang Xu Ying Yun Tingting Jia Zhu Yu |
spellingShingle |
Zhe Wu Shengyang Xu Ying Yun Tingting Jia Zhu Yu Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage Agriculture 3-phenyllactic acid alfalfa silage <i>lactobacillus plantarum</i> strain screening |
author_facet |
Zhe Wu Shengyang Xu Ying Yun Tingting Jia Zhu Yu |
author_sort |
Zhe Wu |
title |
Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage |
title_short |
Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage |
title_full |
Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage |
title_fullStr |
Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage |
title_full_unstemmed |
Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage |
title_sort |
effect of 3-phenyllactic acid and 3-phenyllactic acid-producing lactic acid bacteria on the characteristics of alfalfa silage |
publisher |
MDPI AG |
series |
Agriculture |
issn |
2077-0472 |
publishDate |
2019-12-01 |
description |
In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 10<sup>6</sup> colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as <i>Lactobacillus plantarum</i>. The characteristics of chemical and fermentation compounds indicated that PLA and the two strains efficiently improved the quality of the alfalfa silage. It can be concluded that the use of the strains and PLA can significantly improve the quality of silage. |
topic |
3-phenyllactic acid alfalfa silage <i>lactobacillus plantarum</i> strain screening |
url |
https://www.mdpi.com/2077-0472/10/1/10 |
work_keys_str_mv |
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