Biji kopi meningkatkan ekspresi glutathione padamonosit secara in vitro (Coffee beans increase glutathione expression to monocyes in vitro)

• Main Author: Roedy Budirahardjo
• Format: Article
• Language: English
• Published: Hasanuddin University 2015-02-01
• Series: Journal of Dentomaxillofacial Science
• Subjects: coffee, GSH, antioxidant, immunocytochemistry
• Online Access: http://jdmfs.org/index.php/jdmfs/article/view/428

Some research stated that coffee may act as an antioxidant, but has not mentioned concentrations can distinguish the production of antioxidants. One of the important antioxidant roles in preventing various diseases is GSH. The research is intended to analyze the expression of antioxidant GSH of coffee beans in vitro. Furthermore, the culture of peripheral blood monocytes of healthy people as much as 6 cc, mixed with the anticoagulant heparin. Ficoll-Hypaque centrifugation and suspended in RPMI 1640 medium Buffy coat separated, washed, resuspended. Cells were placed on plate 96-well microtiter of 8 x 105 cells/well, and incubated. Then added RPMI, incubated and lymphocytes discarded. After washing, complete medium was added and incubation. Monocytes reacted with coffee with various concentrations (4 groups) and incubated 18 hours. GSH then analyzed by immunocytochemistry method. GSH cells expressing brown and counted under a light microscope (400x). The results indicated that a concentration of 3 g coffee brewed in 300 mL of hot water has the greatest ability to increase GSH. It was concluded that coffee can improve GSHexpression on monocytes were exposed to DMBA, and the higher the concentration of the coffee bean, the higher the expression of GSH.