Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.
As shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme. Here, we show that PDE3B is activated in response to hig...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2995729?pdf=render |
id |
doaj-8cc7946781f34417b2248ac3b121c812 |
---|---|
record_format |
Article |
spelling |
doaj-8cc7946781f34417b2248ac3b121c8122020-11-25T02:36:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01512e1419110.1371/journal.pone.0014191Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.Emilia HeimannHelena A JonesSvante ResjöVincent C ManganielloLena StensonEva DegermanAs shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme. Here, we show that PDE3B is activated in response to high glucose, insulin and cAMP elevation in rat pancreatic islets and INS-1 (832/13) cells. Activation by glucose was not affected by the presence of diazoxide. PDE3B activation was coupled to an increase as well as a decrease in total phosphorylation of the enzyme. In addition to PDE3B, several other PDEs were detected in human pancreatic islets: PDE1, PDE3, PDE4C, PDE7A, PDE8A and PDE10A. We conclude that PDE3B is activated in response to agents relevant for β-cell function and that activation is linked to increased as well as decreased phosphorylation of the enzyme. Moreover, we conclude that several PDEs are present in human pancreatic islets.http://europepmc.org/articles/PMC2995729?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emilia Heimann Helena A Jones Svante Resjö Vincent C Manganiello Lena Stenson Eva Degerman |
spellingShingle |
Emilia Heimann Helena A Jones Svante Resjö Vincent C Manganiello Lena Stenson Eva Degerman Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. PLoS ONE |
author_facet |
Emilia Heimann Helena A Jones Svante Resjö Vincent C Manganiello Lena Stenson Eva Degerman |
author_sort |
Emilia Heimann |
title |
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. |
title_short |
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. |
title_full |
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. |
title_fullStr |
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. |
title_full_unstemmed |
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. |
title_sort |
expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-01-01 |
description |
As shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme. Here, we show that PDE3B is activated in response to high glucose, insulin and cAMP elevation in rat pancreatic islets and INS-1 (832/13) cells. Activation by glucose was not affected by the presence of diazoxide. PDE3B activation was coupled to an increase as well as a decrease in total phosphorylation of the enzyme. In addition to PDE3B, several other PDEs were detected in human pancreatic islets: PDE1, PDE3, PDE4C, PDE7A, PDE8A and PDE10A. We conclude that PDE3B is activated in response to agents relevant for β-cell function and that activation is linked to increased as well as decreased phosphorylation of the enzyme. Moreover, we conclude that several PDEs are present in human pancreatic islets. |
url |
http://europepmc.org/articles/PMC2995729?pdf=render |
work_keys_str_mv |
AT emiliaheimann expressionandregulationofcyclicnucleotidephosphodiesterasesinhumanandratpancreaticislets AT helenaajones expressionandregulationofcyclicnucleotidephosphodiesterasesinhumanandratpancreaticislets AT svanteresjo expressionandregulationofcyclicnucleotidephosphodiesterasesinhumanandratpancreaticislets AT vincentcmanganiello expressionandregulationofcyclicnucleotidephosphodiesterasesinhumanandratpancreaticislets AT lenastenson expressionandregulationofcyclicnucleotidephosphodiesterasesinhumanandratpancreaticislets AT evadegerman expressionandregulationofcyclicnucleotidephosphodiesterasesinhumanandratpancreaticislets |
_version_ |
1724800240703242240 |