Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors
Abstract Background Meningiomas are the second most common primary tumors of the central nervous system. However, there is a paucity of data on meningioma biology due to the lack of suitable preclinical in vitro and in vivo models. In this study, we report the establishment and characterization of p...
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doaj-8cd633c1aced4040b44d343c2011fc1c2020-11-25T01:19:11ZengBMCCancer Cell International1475-28672020-07-0120111510.1186/s12935-020-01438-xCharacterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumorsEunhye Kim0Mirae Kim1Kyungha So2Young Seok Park3Chang Gok Woo4Sang-Hwan Hyun5Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National UniversityLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National UniversityLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National UniversityDepartment of Neurosurgery, Chungbuk National University Hospital, Chungbuk National University, College of MedicineDepartment of Pathology, Chungbuk National University Hospital, Chungbuk National University, College of MedicineLaboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National UniversityAbstract Background Meningiomas are the second most common primary tumors of the central nervous system. However, there is a paucity of data on meningioma biology due to the lack of suitable preclinical in vitro and in vivo models. In this study, we report the establishment and characterization of patient-derived, spontaneously immortalized cancer cell lines derived from World Health Organization (WHO) grade I and atypical WHO grade II meningiomas. Methods We evaluated high-resolution 3T MRI neuroimaging findings in meningioma patients which were followed by histological analysis. RT-qPCR and immunostaining analyses were performed to determine the expression levels of meningioma-related factors. Additionally, flow cytometry and sorting assays were conducted to investigate and isolate the CD133 and CD44 positive cells from primary atypical meningioma cells. Further, we compared the gene expression profiles of meningiomas and cell lines derived from them by performing whole-exome sequencing of the blood and tumor samples from the patients, and the primary cancer cell lines established from the meningioma tumor. Results Our results were consistent with earlier studies that reported mutations in NF2, SMO, and AKT1 genes in atypical meningiomas, and we also observed mutations in MYBL2, a gene that was recently discovered. Significantly, the genomic signature was consistent between the atypical meningioma cancer cell lines and the tumor and blood samples from the patient. Conclusion Our results lead us to conclude that established meningioma cell lines with a genomic signature identical to tumors might be a valuable tool for understanding meningioma tumor biology, and for screening therapeutic agents to treat recurrent meningiomas.http://link.springer.com/article/10.1186/s12935-020-01438-xAtypical meningiomaPrimary cancer cell lineWhole-exome sequencingStem cell |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eunhye Kim Mirae Kim Kyungha So Young Seok Park Chang Gok Woo Sang-Hwan Hyun |
spellingShingle |
Eunhye Kim Mirae Kim Kyungha So Young Seok Park Chang Gok Woo Sang-Hwan Hyun Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors Cancer Cell International Atypical meningioma Primary cancer cell line Whole-exome sequencing Stem cell |
author_facet |
Eunhye Kim Mirae Kim Kyungha So Young Seok Park Chang Gok Woo Sang-Hwan Hyun |
author_sort |
Eunhye Kim |
title |
Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors |
title_short |
Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors |
title_full |
Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors |
title_fullStr |
Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors |
title_full_unstemmed |
Characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors |
title_sort |
characterization and comparison of genomic profiles between primary cancer cell lines and parent atypical meningioma tumors |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2020-07-01 |
description |
Abstract Background Meningiomas are the second most common primary tumors of the central nervous system. However, there is a paucity of data on meningioma biology due to the lack of suitable preclinical in vitro and in vivo models. In this study, we report the establishment and characterization of patient-derived, spontaneously immortalized cancer cell lines derived from World Health Organization (WHO) grade I and atypical WHO grade II meningiomas. Methods We evaluated high-resolution 3T MRI neuroimaging findings in meningioma patients which were followed by histological analysis. RT-qPCR and immunostaining analyses were performed to determine the expression levels of meningioma-related factors. Additionally, flow cytometry and sorting assays were conducted to investigate and isolate the CD133 and CD44 positive cells from primary atypical meningioma cells. Further, we compared the gene expression profiles of meningiomas and cell lines derived from them by performing whole-exome sequencing of the blood and tumor samples from the patients, and the primary cancer cell lines established from the meningioma tumor. Results Our results were consistent with earlier studies that reported mutations in NF2, SMO, and AKT1 genes in atypical meningiomas, and we also observed mutations in MYBL2, a gene that was recently discovered. Significantly, the genomic signature was consistent between the atypical meningioma cancer cell lines and the tumor and blood samples from the patient. Conclusion Our results lead us to conclude that established meningioma cell lines with a genomic signature identical to tumors might be a valuable tool for understanding meningioma tumor biology, and for screening therapeutic agents to treat recurrent meningiomas. |
topic |
Atypical meningioma Primary cancer cell line Whole-exome sequencing Stem cell |
url |
http://link.springer.com/article/10.1186/s12935-020-01438-x |
work_keys_str_mv |
AT eunhyekim characterizationandcomparisonofgenomicprofilesbetweenprimarycancercelllinesandparentatypicalmeningiomatumors AT miraekim characterizationandcomparisonofgenomicprofilesbetweenprimarycancercelllinesandparentatypicalmeningiomatumors AT kyunghaso characterizationandcomparisonofgenomicprofilesbetweenprimarycancercelllinesandparentatypicalmeningiomatumors AT youngseokpark characterizationandcomparisonofgenomicprofilesbetweenprimarycancercelllinesandparentatypicalmeningiomatumors AT changgokwoo characterizationandcomparisonofgenomicprofilesbetweenprimarycancercelllinesandparentatypicalmeningiomatumors AT sanghwanhyun characterizationandcomparisonofgenomicprofilesbetweenprimarycancercelllinesandparentatypicalmeningiomatumors |
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