Involvement of opioid system in behavioral despair induced by social isolation stress in mice
Social isolation stress (SIS) as a type of chronic stress could induce depressive- and anxiety-like behaviors. Our study evaluates the role of opioid system on negative behavioral impacts of SIS in male NMRI mice. We investigated effects of morphine, a nonselective opioid receptor (OR) agonist, nalt...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2019-01-01
|
Series: | Biomedicine & Pharmacotherapy |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332218340162 |
id |
doaj-8ce8a2f84fed4c54bb0e5bc10d4dad35 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arvin Haj-Mirzaian Rajan Nikbakhsh Kiana Ramezanzadeh Mehdi Rezaee Hossein Amini-Khoei Arya Haj-Mirzaian Maria Ghesmati Khashayar Afshari Nazgol-Sadat Haddadi Ahmad Reza Dehpour |
spellingShingle |
Arvin Haj-Mirzaian Rajan Nikbakhsh Kiana Ramezanzadeh Mehdi Rezaee Hossein Amini-Khoei Arya Haj-Mirzaian Maria Ghesmati Khashayar Afshari Nazgol-Sadat Haddadi Ahmad Reza Dehpour Involvement of opioid system in behavioral despair induced by social isolation stress in mice Biomedicine & Pharmacotherapy Social isolation stress Opioid Behavioral despair Seizure |
author_facet |
Arvin Haj-Mirzaian Rajan Nikbakhsh Kiana Ramezanzadeh Mehdi Rezaee Hossein Amini-Khoei Arya Haj-Mirzaian Maria Ghesmati Khashayar Afshari Nazgol-Sadat Haddadi Ahmad Reza Dehpour |
author_sort |
Arvin Haj-Mirzaian |
title |
Involvement of opioid system in behavioral despair induced by social isolation stress in mice |
title_short |
Involvement of opioid system in behavioral despair induced by social isolation stress in mice |
title_full |
Involvement of opioid system in behavioral despair induced by social isolation stress in mice |
title_fullStr |
Involvement of opioid system in behavioral despair induced by social isolation stress in mice |
title_full_unstemmed |
Involvement of opioid system in behavioral despair induced by social isolation stress in mice |
title_sort |
involvement of opioid system in behavioral despair induced by social isolation stress in mice |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2019-01-01 |
description |
Social isolation stress (SIS) as a type of chronic stress could induce depressive- and anxiety-like behaviors. Our study evaluates the role of opioid system on negative behavioral impacts of SIS in male NMRI mice. We investigated effects of morphine, a nonselective opioid receptor (OR) agonist, naltrexone (NLX), an OR antagonist, naltrindole (NLT), a delta opioid receptor (DOR) antagonist, SNC80, a DOR agonist, U-69593, a kappa opioid receptor (KOR) agonist, nor-Binaltorphimine, a selective KOR antagonist and cyprodime hydrochloride a selective mu opioid receptor (MOR) antagonist on depressive- and anxiety-like behaviors. Using RT-PCR we evaluated ORs gene expression in mice brain. Our findings showed that SIS induced anxiety- and depressive-like behavior in the forced swimming test, open field test, splash test and hole-board test. Moreover, administration of SNC-80 significantly mitigated anxiety- and depressive-like behaviors. NLT decreased grooming-activity in the splash test. Excitingly, administration of agents affecting KOR failed to alter the negative effects of SIS. RT-PCR demonstrated that MOR and KOR gene expression decreased in socially isolated mice; however, SIS did not affect DORs expression. Our findings suggest that SIS at least in part, probably via altering endogenous opioids particularly MORs and KORs but not DORs mediated negative impacts on behavior; also, it could be concluded that DORs might be considered as a novel target for studying depression and anxiety. |
topic |
Social isolation stress Opioid Behavioral despair Seizure |
url |
http://www.sciencedirect.com/science/article/pii/S0753332218340162 |
work_keys_str_mv |
AT arvinhajmirzaian involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT rajannikbakhsh involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT kianaramezanzadeh involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT mehdirezaee involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT hosseinaminikhoei involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT aryahajmirzaian involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT mariaghesmati involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT khashayarafshari involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT nazgolsadathaddadi involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice AT ahmadrezadehpour involvementofopioidsysteminbehavioraldespairinducedbysocialisolationstressinmice |
_version_ |
1721433136607264768 |
spelling |
doaj-8ce8a2f84fed4c54bb0e5bc10d4dad352021-05-21T04:15:59ZengElsevierBiomedicine & Pharmacotherapy0753-33222019-01-01109938944Involvement of opioid system in behavioral despair induced by social isolation stress in miceArvin Haj-Mirzaian0Rajan Nikbakhsh1Kiana Ramezanzadeh2Mehdi Rezaee3Hossein Amini-Khoei4Arya Haj-Mirzaian5Maria Ghesmati6Khashayar Afshari7Nazgol-Sadat Haddadi8Ahmad Reza Dehpour9Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Anesthesiology, Critical Care and Pain Medicine, Tehran University of Medical Science, Tehran, IranMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran; Department of Physiology and Pharmacology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Microbiology, Islamic Azad University of Lahijan Branch, Lahijan, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Corresponding author at: Experimental Medicine Research Center, Tehran University of Medical Sciences, P.O.Box 13145-784, Tehran, Iran.Social isolation stress (SIS) as a type of chronic stress could induce depressive- and anxiety-like behaviors. Our study evaluates the role of opioid system on negative behavioral impacts of SIS in male NMRI mice. We investigated effects of morphine, a nonselective opioid receptor (OR) agonist, naltrexone (NLX), an OR antagonist, naltrindole (NLT), a delta opioid receptor (DOR) antagonist, SNC80, a DOR agonist, U-69593, a kappa opioid receptor (KOR) agonist, nor-Binaltorphimine, a selective KOR antagonist and cyprodime hydrochloride a selective mu opioid receptor (MOR) antagonist on depressive- and anxiety-like behaviors. Using RT-PCR we evaluated ORs gene expression in mice brain. Our findings showed that SIS induced anxiety- and depressive-like behavior in the forced swimming test, open field test, splash test and hole-board test. Moreover, administration of SNC-80 significantly mitigated anxiety- and depressive-like behaviors. NLT decreased grooming-activity in the splash test. Excitingly, administration of agents affecting KOR failed to alter the negative effects of SIS. RT-PCR demonstrated that MOR and KOR gene expression decreased in socially isolated mice; however, SIS did not affect DORs expression. Our findings suggest that SIS at least in part, probably via altering endogenous opioids particularly MORs and KORs but not DORs mediated negative impacts on behavior; also, it could be concluded that DORs might be considered as a novel target for studying depression and anxiety.http://www.sciencedirect.com/science/article/pii/S0753332218340162Social isolation stressOpioidBehavioral despairSeizure |