Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State, Nigeria. A total of six hundred and five (605) samples were collected asepticall...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Rabie Fayed
2021-10-01
|
Series: | Journal of Applied Veterinary Sciences |
Subjects: | |
Online Access: | https://javs.journals.ekb.eg/article_192695.html |
id |
doaj-8cf06a6c9a954f25963950fea33feb83 |
---|---|
record_format |
Article |
spelling |
doaj-8cf06a6c9a954f25963950fea33feb832021-10-07T12:43:35ZengRabie FayedJournal of Applied Veterinary Sciences1687-40722090-33082021-10-01643238https://dx.doi.org/10.21608/javs.2021.88440.1094Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, NigeriaOnyilokwu, S. A.0Adamu, S. G.1https://orcid.org/0000-0003-4066-3127Lawan, F. A.2Aye, A. M.3Department of Medical Laboratory Sciences, Faculty of Allied and Health Sciences, University of Maiduguri, P.M.B. 1069 Maiduguri Borno State, Nigeria.Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Maiduguri, P.M.B. 1069 Maiduguri Borno State, Nigeria.Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Maiduguri, P.M.B. 1069 Maiduguri, Borno State, Nigeria.Zankli Medical Service, No.1 Ibrahim Tahir Street Mabushi Opposite Federal Ministry of works, Abuja, Nigeria.This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State, Nigeria. A total of six hundred and five (605) samples were collected aseptically using sterile swab sticks (Oxoid, UK) using a simple random technique from the ruminants and their human handlers at the Maiduguri central abattoir and livestock market (Kasuwan shanu). One hundred and fifty-one (151) samples were collected from each species (cattle, sheep and goat), while 152 samples were collected from animal handlers. The samples were immediately subjected to standard bacteriological analysis. A total of 195 (32.2%) coagulase-negative Staphylococcus species (CoNS) were presumptively identified; out of the 195 identified CoNS, 9 (4.62%) was Staphylococcus piscifermentans nasal colonization. The results revealed that out of 9 isolates, S. piscifermentans was highly isolated 4(8.3%) from sheep, followed by cattle and human handlers 2 (3.8%) respectively, and the least was from goats 1 (2.3%). Meanwhile, all the 9 (4.62%) isolates were negative for both tube and slide coagulase test. However, they were positive for DNase, Ornithine Decarboxylase (ODC) test, betta – hemolysis and synergistic hemolysis. 4(44.4%) of the 9 isolates were positive for Staphylococcus Protein A (SPA) virulence gene, none 0(0%) for mecA virulence gene, and 5(55.6%) were positive for slime (Biofilm) production. The antibiotic susceptibility pattern of these isolates was found to be highly variable to all the used drugs. Therefore, it is concluded that Staphylococcus piscifermentans isolated from these ruminants and their handlers contain virulence genes that could be responsible for pathogenicity. There is a need for more studies using higher technologies in other parts of the country to reveal the thorough status of the disease in Nigeria.https://javs.journals.ekb.eg/article_192695.htmlhandlersoccurrencepiscifermentansruminantssusceptibility profiles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Onyilokwu, S. A. Adamu, S. G. Lawan, F. A. Aye, A. M. |
spellingShingle |
Onyilokwu, S. A. Adamu, S. G. Lawan, F. A. Aye, A. M. Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria Journal of Applied Veterinary Sciences handlers occurrence piscifermentans ruminants susceptibility profiles |
author_facet |
Onyilokwu, S. A. Adamu, S. G. Lawan, F. A. Aye, A. M. |
author_sort |
Onyilokwu, S. A. |
title |
Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria |
title_short |
Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria |
title_full |
Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria |
title_fullStr |
Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria |
title_full_unstemmed |
Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria |
title_sort |
occurrence of staphylococcus piscifermentans among apparently healthy ruminants and their handlers in maiduguri, borno state, nigeria |
publisher |
Rabie Fayed |
series |
Journal of Applied Veterinary Sciences |
issn |
1687-4072 2090-3308 |
publishDate |
2021-10-01 |
description |
This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State, Nigeria. A total of six hundred and five (605) samples were collected aseptically using sterile swab sticks (Oxoid, UK) using a simple random technique from the ruminants and their human handlers at the Maiduguri central abattoir and livestock market (Kasuwan shanu). One hundred and fifty-one (151) samples were collected from each species (cattle, sheep and goat), while 152 samples were collected from animal handlers. The samples were immediately subjected to standard bacteriological analysis. A total of 195 (32.2%) coagulase-negative Staphylococcus species (CoNS) were presumptively identified; out of the 195 identified CoNS, 9 (4.62%) was Staphylococcus piscifermentans nasal colonization. The results revealed that out of 9 isolates, S. piscifermentans was highly isolated 4(8.3%) from sheep, followed by cattle and human handlers 2 (3.8%) respectively, and the least was from goats 1 (2.3%). Meanwhile, all the 9 (4.62%) isolates were negative for both tube and slide coagulase test. However, they were positive for DNase, Ornithine Decarboxylase (ODC) test, betta – hemolysis and synergistic hemolysis. 4(44.4%) of the 9 isolates were positive for Staphylococcus Protein A (SPA) virulence gene, none 0(0%) for mecA virulence gene, and 5(55.6%) were positive for slime (Biofilm) production. The antibiotic susceptibility pattern of these isolates was found to be highly variable to all the used drugs. Therefore, it is concluded that Staphylococcus piscifermentans isolated from these ruminants and their handlers contain virulence genes that could be responsible for pathogenicity. There is a need for more studies using higher technologies in other parts of the country to reveal the thorough status of the disease in Nigeria. |
topic |
handlers occurrence piscifermentans ruminants susceptibility profiles |
url |
https://javs.journals.ekb.eg/article_192695.html |
work_keys_str_mv |
AT onyilokwusa occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria AT adamusg occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria AT lawanfa occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria AT ayeam occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria |
_version_ |
1716839335277363200 |